Search results for " culture"

SV40 transformed fibroblasts recognize the same 140 kD fibronectin chemotactic fragment as non-transformed cells

1985

SV40-virus-transformed human embryonal fibroblasts show an enhanced chemotactic response to the glycoprotein fibronectin. However, they recognize the same chemotactic active region as non-transformed fibroblasts. The result suggests that an enhancement of chemotaxis by fibroblasts which have been transformed with Simian Virus 40 is due not to the utilization of further chemotactic domains in the molecule, but to an increased sensitivity of the cells to the chemoattractant.

Oncolytic targeting of renal cell carcinoma via encephalomyocarditis virus

2010

Apoptosis is a fundamental host defence mechanism against invading microbes. Inactivation of NF-kappaB attenuates encephalomyocarditis virus (EMCV) virulence by triggering rapid apoptosis of infected cells, thereby pre-emptively limiting viral replication. Recent evidence has shown that hypoxia-inducible factor (HIF) increases NF-kappaB-mediated anti-apoptotic response in clear-cell renal cell carcinoma (CCRCC) that commonly exhibit hyperactivation of HIF due to the loss of its principal negative regulator, von Hippel-Lindau (VHL) tumour suppressor protein. Here, we show that EMCV challenge induces a strong NF-kappaB-dependent gene expression profile concomitant with a lack of interferon-me…

PTC124-mediated translational readthrough of a nonsense mutation causing Usher syndrome type 1C.

2011

We investigated the therapeutic potential of the premature termination codon (PTC) readthrough-inducing drug PTC124 in treating the retinal phenotype of Usher syndrome, caused by a nonsense mutation in the USH1C gene. Applications in cell culture, organotypic retina cultures, and mice in vivo revealed significant readthrough and the recovery of protein function. In comparison with other readthrough drugs, namely the clinically approved readthrough-inducing aminoglycoside gentamicin, PTC124 exhibits significant better retinal biocompatibility. Its high readthrough efficiency in combination with excellent biocompatibility makes PTC124 a promising therapeutic agent for PTCs in USH1C, as well a…

Characterization of the autoantigen La (SS-B) as a dsRNA unwinding enzyme

1997

During the analysis of the La (SS-B) autoantigen for catalytic activities an ATP-dependent double-stranded RNA unwinding activity was detected. Both native and recombinant La proteins from different species displayed this activity, which could be inhibited by monospecific anti-La antibodies. La protein was able to melt dsRNA substrates with either two 3'-overhangs or a single 3'- and a 5'-overhang. Double-stranded RNAs with two 5'-overhangs were not unwound, indicating that at least one 3'-overhang is required for unwinding. Sequence elements of the La protein that might be involved in dsRNA unwinding, such as an evolutionarily conserved putative ATP-binding motif and an element that is hom…

Clathrin- and Caveolin-Independent Entry of Human Papillomavirus Type 16—Involvement of Tetraspanin-Enriched Microdomains (TEMs)

2008

BACKGROUND: Infectious entry of human papillomaviruses into their host cells is an important step in the viral life cycle. For cell binding these viruses use proteoglycans as initial attachment sites. Subsequent transfer to a secondary receptor molecule seems to be involved in virus uptake. Depending on the papillomavirus subtype, it has been reported that entry occurs by clathrin- or caveolin-mediated mechanisms. Regarding human papillomavirus type 16 (HPV16), the primary etiologic agent for development of cervical cancer, clathrin-mediated endocytosis was described as infectious entry pathway. METHODOLOGY/PRINCIPAL FINDINGS: Using immunofluorescence and infection studies we show in contra…

Cultura visuale. Una genealogia

2020

Questo libro offre una ricognizione delle principali genealogie novecentesche della cultura visuale, scegliendo solo tre figure paradigmatiche: Aby Warburg, Sigmund Freud e Walter Benjamin. Dopo Warburg la nozione di immagine è definitivamente cambiata. Dopo Freud lo sguardo è indissolubilmente legato al tema della sessualità e del bios. Dopo Benjamin la nozione di dispositivo e quelle di apparato, assemblage e ambiente mediale sono definitivamente cambiate. Questi autori non stanno solo alle origini della cultura visuale ma ne prefigurano gli sviluppi futuri. Da Warburg abbiamo imparato che il fare-immagine fa parte della biologia e dei comportamenti essenziali dell’Homo sapiens, un aspett…

How secondary school pupils bring their informal learning experiences into a Content and Language Integrated (CLIL) classroom

2014

This article explores how students’ informal language learning experiences with English find their way into the formal context of content-based language teaching (CLIL). The analysis is focused on stretches of classroom talk in which native Finnish-speaking students draw on their expertise of English-language popular culture, and use their knowledge as a semiotic resource for producing various types of actions. Based on the data, it is argued that the organisation of peer group talk in the language classroom provides students with affordances for participation that are characteristically different from whole-class interaction. In this environment, global popular culture and conversational h…

Comparison of the mycelial growth of some Phellinus spp. isolates on different agar media

2017

Celem pracy było określenie i porównanie wzrostu grzybni Phellinus spp. Użyto dziewięciu izolatów z gatunków: P. torulosus, P. robustus, P. pomaceus, P. igniarius i P. pini, które uprawiano na czterech różnych pożywkach agarowych, aby określić ich przydatność dla rodzaju Phellinus. Zastosowano pożywki: ziemniaczano-glukozową (PDA), maltozową (MEA), pszenną (WA) i trocinowo-pszenną (SWA). Po 10 dniach inkubacji w 25°C zmierzono średnice kolonii. Artykuł prezentuje dane dotyczące wzrostu grzybni (G) i krótkie opisy koloni na MEA. Wszystkie izolaty wykazały mały wzrost na MEA i duży wzrost na WA, SWA i PDA. Wzrost grzybni wszystkich izolatów na poszczególnych pożywkach wyniósł odpowiednio: MEA…

Taikabeibejä ja kuulapsia : noituutta YouTubessa

2019

Longitudinal Patterns of Ethical Organisational Culture as a Context for Leaders’ Well-Being: Cumulative Effects Over 6 Years

2021

AbstractThe aim of this longitudinal study was to investigate the temporal dynamics of ethical organisational culture and how it associates with well-being at work when potential changes in ethical culture are measured over an extended period of 6 years. We used a person-centred study design, which allowed us to detect both typical and atypical patterns of ethical culture stability as well as change among a sample of leaders. Based on latent profile analysis and hierarchical linear modelling we found longitudinal, concurrent relations and cumulative gain and loss cycles between different ethical culture patterns and leaders’ well-being. Leaders in the strongest ethical culture pattern exper…