Search results for " culture"
showing 10 items of 5779 documents
Tobacco cells contain a protein, immunologically related to the neutrophil small G protein Rac2 and involved in elicitor-induced oxidative burst.
1997
Abstract Suspension-cultured cells of Nicotiana tabacum generated active oxygen species (AOS) when they were treated with the proteinaceous elicitor, cryptogein. This response was blocked by diphenylene iodonium, an inhibitor of the neutrophil NADPH oxidase. When microsomal extracts of tobacco cells were probed with an antibody directed against the human small G protein Rac2, two immunoreactive proteins were detected at 18.5 and 20.5 kDa. The same experiment performed with cytosolic extracts of tobacco cells led to the observation of a strong immunoreactive protein at 21.5 kDa only in the cryptogein-treated cells. The appearance of this cytosolic protein was related to the production of AOS…
Hydrogen peroxide induces programmed cell death features in cultured tobacco BY-2 cells, in a dose-dependent manner
2001
Active oxygen species (AOS), especially hydrogen peroxide, play a critical role in the defence of plants against invading pathogens and in the hypersensitive response (HR). This is characterized by the induction of a massive production of AOS and the rapid appearance of necrotic lesions is considered as a programmed cell death (PCD) process during which a limited number of cells die at the site of infection. This work was aimed at investigating the mode of cell death observed in cultures of BY-2 tobacco cells exposed to H(2)O(2). It was shown that H(2)O(2) is able to induce various morphological cell death features in cultured tobacco BY-2 cells. The hallmarks of cell death observed with fl…
Involvement of putative glutamate receptors in plant defence signaling and NO production
2011
International audience; Ionotropic glutamate receptors (iGluRs) are non-selective cation channels permeable to calcium, present in animals and plants. In mammals, glutamate is a well-known neurotransmitter and recently has been recognized as an immunomodulator. As animals and plants share common mechanisms that govern innate immunity with calcium playing a key role in plant defence activation, we have checked the involvement of putative iGluRs in plant defence signaling. Using tobacco cells, we first provide evidence supporting the activity of iGluRs as calcium channels and their involvement in NO production as reported in animals. Thereafter, iGluRs were shown to be activated in response t…
Different bacteria can enhance the micropropagation response of Cotoneaster lacteus (Rosaceae)
1998
C. MONIER, E. BOSSIS, C. CHABANET AND R. SAMSON. 1998. Cotoneaster species are widely used ornamental shrubs, and research is under way to select genotypes which are more resistant to fire blight by somaclonal variation. During the establishment of micropropagation, one genotype, Cotoneaster lacteus, showed the development of endogenous bacterial contaminants. Two strains were isolated from in vitro explants: Bacillus pumilus and Alcaligenes faecalis. These two strains, and some Pseudomonas fluorescens and Ps. putida, promoted in vitro multiplication and rooting of explants. In Pseudomonas strains, rooting enhancing capacity appeared to be related to indole-3-acetic acid (IAA) production, a…
Characterizing the effects of salt stress in Calendula officinalis L
2017
In this study the effects of salt stress on growth and several stress markers were investigated in the ornamental and medicinal plant Calendula officinalis. One month old plants were submitted to increasing concentrations of salt up to 150mM NaCl for a period of 30 days. Salinity affected growth in terms of stem length and fresh weight of the plants, but water content remained unchanged indicating a certain tolerance to low and mild concentrations of salt. Although Na+ and Cl− increased in parallel to applied salt treatments, the levels of K+ and Ca2+ showed no significant change, while Mg2+ levels recorded a two folds increase upon the application of the highest salt concentration. Other m…
Water-Related Variables for Predicting Yield of Apple under Deficit Irrigation
2019
, it did not reduce yield and gas exchange. In &lsquo
A Cultivar-Sensitive Approach for the Continuous Monitoring of Olive (Olea europaea L.) Tree Water Status by Fruit and Leaf Sensing
2020
Sustainable irrigation is crucial to reduce water use and management costs in modern orchard systems. Continuous plant-based sensing is an innovative approach for the continuous monitoring of plant water status. Olive (Olea europaea L.) genotypes can respond to drought using different leaf and fruit physiological and morphological mechanisms. This study aimed to identify whether fruit and leaf water dynamics of two different olive cultivars were differently affected by water deficit and their response to changes of midday stem water potential (Ψstem), the most common indicator of plant water status. Plant water status indicators such as leaf stomatal conductance (gs) and Ψstem were measured…
Effects of Three Irrigation Strategies on Gas Exchange Relationships, Plant Water Status, Yield Components and Water Productivity on Grafted Carménèr…
2018
In the Chilean viticultural industry, Carménère is considered an emblematic cultivar that is cultivated mainly in arid and semi-arid zones. For this reason, it is necessary to use precise irrigation scheduling for improving water use efficiency (WUE), water productivity (WP), yield and wine quality. This study evaluated the effects of three deficit irrigation strategies on gas exchange variables, WUE, WP and yield components in a drip-irrigated Carménère vineyard growing under semi-arid climatic conditions during two consecutive seasons (2011/12 and 2012/13). The irrigation strategies were applied in completely randomized design from fruit set (S) to harvest (H). The first irrigation strate…
Nitrogen Type and Availability Drive Mycorrhizal Effects on Wheat Performance, Nitrogen Uptake and Recovery, and Production Sustainability
2020
Plant performance is strongly dependent on nitrogen (N), and thus increasing N nutrition is of great relevance for the productivity of agroecosystems. The effects of arbuscular mycorrhizal (AM) fungi on plant N acquisition are debated because contradictory results have been reported. Using 15N-labeled fertilizers as a tracer, we evaluated the effects of AM fungi on N uptake and recovery from mineral or organic sources in durum wheat. Under sufficient N availability, AM fungi had no effects on plant biomass but increased N concentrations in plant tissue, plant N uptake, and total N recovered from the fertilizer. In N-deficient soil, AM fungi led to decreased aboveground biomass, which sugges…
AM fungal exudates activate MAP kinases in plant cells in dependence from cytosolic Ca2+ increase
2011
International audience; The molecular dialogue occurring prior to direct contact between the fungal and plant partners of arbuscular-mycorrhizal (AM) symbioses begins with the release of fungal elicitors, so far only partially identified chemically, which can activate specific signaling pathways in the host plant. We show here that the activation of MAPK is also induced by exudates of germinating spores of Gigaspora margarita in cultured cells of the non-leguminous species tobacco (Nicotiana tabacum), as well as in those of the model legume Lotus japonicus. MAPK activity peaked about 15 min after the exposure of the host cells to the fungal exudates (FE). FE were also responsible for a rapi…