Search results for " data"
showing 10 items of 7516 documents
Downregulation of a Chitin Deacetylase-Like Protein in Response to Baculovirus Infection and Its Application for Improving Baculovirus Infectivity
2009
ABSTRACT Several expressed sequence tags (ESTs) with homology to chitin deacetylase-like protein (CDA) were selected from a group of Helicoverpa armigera genes whose expression changed after infection with H. armigera single nucleopolyhedrovirus (HearNPV). Some of these ESTs coded for a midgut protein containing a chitin deacetylase domain (CDAD). The expressed protein, HaCDA5a, did not show chitin deacetylase activity, but it showed a strong affinity for binding to chitin. Sequence analysis showed the lack of any chitin binding domain, described for all currently known peritrophic membrane (PM) proteins. HaCDA5a has previously been detected in the H. armigera PM. Such localization, togethe…
BlotBase: a northern blot database.
2008
With the availability of high-throughput gene expression analysis, multiple public expression databases emerged, mostly based on microarray expression data. Although these databases are of significant biomedical value, they do hold significant drawbacks, especially concerning the reliability of single gene expression profiles obtained by microarray data. Simultaneously, reliable data on an individual gene's expression are often published as single northern blots in individual publications. These data were not yet available for high-throughput screening. To reduce the gap between high-throughput expression data and individual highly reliable expression data, we designed a novel database "Blo…
Measurements of baryon pair decays of chi(cJ) mesons
2013
Using 106 $\times 10^{6}$ $\psi^{\prime}$ decays collected with the BESIII detector at the BEPCII, three decays of $\chi_{cJ}$ ($J=0,1,2$) with baryon pairs ($\llb$, $\ssb$, $\SSB$) in the final state have been studied. The branching fractions are measured to be $\cal{B}$$(\chi_{c0,1,2}\rightarrow\Lambda\bar\Lambda) =(33.3 \pm 2.0 \pm 2.6)\times 10^{-5}$, $(12.2 \pm 1.1 \pm 1.1)\times 10^{-5}$, $(20.8 \pm 1.6 \pm 2.3)\times 10^{-5}$; $\cal{B}$$(\chi_{c0,1,2}\rightarrow\Sigma^{0}\bar\Sigma^{0})$ = $(47.8 \pm 3.4 \pm 3.9)\times 10^{-5}$, $(3.8 \pm 1.0 \pm 0.5)\times 10^{-5}$, $(4.0 \pm 1.1 \pm 0.5) \times 10^{-5}$; and $\cal{B}$$(\chi_{c0,1,2}\rightarrow\Sigma^{+}\bar\Sigma^{-})$ = $(45.4 \pm…
Some aspects regarding the static behavior of basalt in the machine building industry
2017
Basalt is a material available in big quantities in nature. It has a low price and can be used in various domains. It can be relatively easily extracted from natural deposits and processed by means of melting. Melted and recrystallized basalt has demonstrated promising behavior with regards of its use in manufacturing, as reported in the literature. This work aims to present some aspects regarding the opportunity of using basalt in manufacturing the subassemblies of machine-tools. The research was made using grey cast iron and steel parts as reference for a comparative study. The researches presented in this work were focused on analyzing the behavior of basalt parts submitted to static str…
Mitochondrial DNA Regionalism and Historical Demography in the Extant Populations of Chirocephalus kerkyrensis (Branchiopoda: Anostraca)
2012
BackgroundMediterranean temporary water bodies are important reservoirs of biodiversity and host a unique assemblage of diapausing aquatic invertebrates. These environments are currently vanishing because of increasing human pressure. Chirocephalus kerkyrensis is a fairy shrimp typical of temporary water bodies in Mediterranean plain forests and has undergone a substantial decline in number of populations in recent years due to habitat loss. We assessed patterns of genetic connectivity and phylogeographic history in the seven extant populations of the species from Albania, Corfu Is. (Greece), Southern and Central Italy.Methodology/principal findingsWe analyzed sequence variation at two mito…
Direct detection of repetitive, whole chromosome paint and telomere DNA probes by immunogold electron microscopy
1993
Biotinylated repetitive, whole chromosome paint and telomere DNA probes were investigated at the electron microscope level after non-isotopic in situ hybridization and direct immunogold detection. The protocol described allowed the visualization of a biotinylated chromosome 1 specific satellite DNA probe in the light microscope without silver intensification. This sensitive method was exploited to analyse factors contributing to signal strength in immunogold chromosome painting. Furthermore, it allowed us to investigate the distribution of (TTAGGG)n telomere repeats in human metaphase chromosomes and interphase nuclei. Telomeric and internal (TTAGGG)n repeats were detected at high spatial r…
Morphological and molecular taxonomy ofPythium longisporangiumsp. nov. isolated from the Burgundian region of France
2005
During the course of an investigation on the Pythiaceous oomycetes occurring in the Burgundian vineyards, some species of Pythium possessing mainly hypogynous antheridia were found. These had been classified as oomycetes belonging to the ‘‘Pythium rostratum’’ group for a long time. Three of these isolates, having similar structures and growth, are very closely related to a recently described species, Pythium bifurcatum Paul. A close look at these, however, underlines some fundamental differences with the latter. Not all of them produce zoospores but have very large sporangia. The type specimen is F-1200 (B 76a) which is a medium-slow growing saprophyte. The sequence of the ITS region of the…
Cloning and characterization of the phenylalanyl-tRNA synthetase β subunit gene fromCandida albicans
1998
A Candida albicans expression library was constructed from RNA isolated from regenerating protoplasts. A 1.4-kb cDNA clone was used to isolate a genomic fragment. Sequence analysis revealed an open reading frame of 593 amino acids with an overall identity of 63.6% with the phenylalanyl-tRNA synthetase beta subunit (FRS1) of Saccharomyces cerevisiae. We named it CaFRS1. It is located in a single copy in chromosome R, SfiI fragment M. Its expression showed a decrease during the cell wall regeneration process in protoplasts of both yeast and mycelial cells of C. albicans, suggesting its requirement thereof in initial steps of the cell wall synthesis.
Pythium prolatumisolated from soil in the Burgundy region: a new record for Europe
1999
Pythium prolatum Hendrix and Campbell has been isolated from a soil sample taken in the Burgundy region in France. The fungus is easily recognisable by its heavily ornamented oogonia with conical to mammiform spines, elongated sporangia, and its diclinous antheridia forming or originating from a tangled mass of hyphae. Descriptions of the morphological and reproductive aspects of Pythium prolatum, the polymerase chain reaction of the internal transcribed spacer (ITS1) of the ribosomal nuclear DNA as well as the nucleotide sequences of ITS1 coding for 5.8 S rRNA are given.
Pythium segnitiumsp. nov., isolated from the Canary Islands â its taxonomy, ITS region of rDNA, and comparison with related species
2002
Pythium segnitium (CI-44) was isolated from some soil samples taken in the Canary Islands (Spain). This new species is a slow-growing fungus and is perfectly adapted to terrestrial habitat. It belongs to the group of Pythium that have smooth-walled oogonia, mostly hypogynous antheridia, and plerotic oospores. The fungus lacks sporangia, zoospores, and hyphal bodies are rarely formed. Thus the asexual reproduction, which is so common for fungi and especially for the aquatic ones, is completely lacking in this case. However the fungus reproduces sexually by the formation of oogonia, antheridia and oospores plentifully. The taxonomic description of this fungus, the nucleotide sequence of the i…