Search results for " enhancement."
showing 10 items of 513 documents
Editorial: Genetics and gene therapy of lysosomial storage disorders
2018
Non applicabile in quanto editoriale
Hutchinson Gilford Progeria Syndrome: A Therapeutic Approach via Adenoviral Delivery of CRISPR/cas Genome Editing System
2015
Hutchinson-Gilford Progeria Syndrome (HGPS) is a rare human genetic disease caused by mutations in the LMNA gene. LMNA codes for structural components of the nuclear lamina. Alterations of nuclear lamina lead to a very variable class of diseases known as laminopathies. In detail, HGPS manifests a severe premature ageing phenotype due to the accumulation of a dominant negative form of lamin-A called progerin. With current treatments, the life expectancy of HGPS patients does not exceed their second decade. Death is usually due to cardiovascular complications. Recently, a new technology for mammals in vivo gene editing has been developed: the clustered regularly interspaced short palindromic …
In vitro genome editing using CRISPR/Cas9 to edit SERPINA1 PiZ mutation
2019
Introduction: The emergence some years ago of the CRISPR/Cas9 system allowed gene therapy to be specific, versatile, cheap and approachable to almost every laboratory. Due to these features, many different genetic diseases such as cystic fibrosis or β-thalassemia have been addressed in cellular models using the CRISPR/Cas9 genetic editing tool. Alpha-1 antytripsin deficiency (AATD) is a rare genetic condition that can provoke respiratory and hepatic symptoms. The Z allele of SERPINA1 gene is a well-characterised point mutation that can trigger the whole pathology. Henceforth, Z mutation is a suitable target for genetic edition using CRISPR/Cas9 in order to develop a gene therapy to treat AA…
Estimation of Purkinje trees from electro-anatomical mapping of the left ventricle using minimal cost geodesics
2015
The electrical activation of the heart is a complex physiological process that is essential for the understanding of several cardiac dysfunctions, such as ventricular tachycardia (VT). Nowadays, patient-specific activation times on ventricular chambers can be estimated from electro-anatomical maps, providing crucial information to clinicians for guiding cardiac radio-frequency ablation treatment. However, some relevant electrical pathways such as those of the Purkinje system are very difficult to interpret from these maps due to sparsity of data and the limited spatial resolution of the system. We present here a novel method to estimate these fast electrical pathways from the local activati…
Gentherapie solider Tumore
2001
Trotz intensiver therapeutischer Anstrengungen sind Verbesserungen der Uberlebensdaten sowie der Remissionsraten von Patienten mit fortgeschrittenen soliden Tumoren eher die Ausnahme geblieben. In den vergangenen Jahren wurden deshalb mehrere neue Therapiemodalitaten klinisch gepruft, von denen die so genannte “somatische Gentherapie” besondere Aufmerksamkeit erfahren hat. Bei dieser neuartigen Behandlungsform wird mittels verschiedener Vektoren die kodierende Gensequenz eines oder mehrerer Gene in somatische Zellen ubertragen und exprimiert. Hierbei kommen sowohl virale (z. B. Adenoviren, Retroviren) als auch nicht-virale Vektoren (z. B. Liposomen) zum Einsatz. Der vorliegende Beitrag besc…
Tumor enhancement at contrast-enhanced CT and Gd-enhanced MRI for the assessment of treatment response of hepatocellular carcinoma (HCC) after sorafe…
2012
Scopo: To investigate whether arterial enhancement of advanced HCC during pre-treatment and follow-up contrast-enhanced CT (CECT) or Gd-enhanced MRI (Gd-MRI) can be used to predict tumor response to sorafenib. Materiali e metodi: Seventeen patients (12M, 5F; mean age: 69 years) receiving sorafenib for inoperable HCC between 2007 and 2010 were included. Median interval time between pre-treatment and follow-up CECT or Gd-MRI was 160 days. Tumor arterial enhancement was measured at baseline and follow-up: (tumor attenuation/intensity on arterial phase – tumor attenuation/intensity on unenh! anced images)/(tumor attenuation/intensity on unenhanced images) x 100. Response was assessed according …
Soft-dielectron excess in proton-proton collisions at $\sqrt{s}$ = 13 TeV
2021
Physical review letters 127(4), 042302 (2021). doi:10.1103/PhysRevLett.127.042302
Computer-aided detection of cerebral microbleeds in susceptibility-weighted imaging.
2014
Susceptibility-weighted imaging (SWI) is recognized as the preferred MRI technique for visualizing cerebral vasculature and related pathologies such as cerebral microbleeds (CMBs). Manual identification of CMBs is time-consuming, has limited reliability and reproducibility, and is prone to misinterpretation. In this paper, a novel computer-aided microbleed detection technique based on machine learning is presented: First, spherical-like objects (potential CMB candidates) with their corresponding bounding boxes were detected using a novel multi-scale Laplacian of Gaussian technique. A set of robust 3-dimensional Radon- and Hessian-based shape descriptors within each bounding box were then ex…
Depth Enhancement by Fusion for Passive and Active Sensing
2012
This paper presents a general refinement procedure that enhances any given depth map obtained by passive or active sensing. Given a depth map, either estimated by triangulation methods or directly provided by the sensing system, and its corresponding 2-D image, we correct the depth values by separately treating regions with undesired effects such as empty holes, texture copying or edge blurring due to homogeneous regions, occlusions, and shadowing. In this work, we use recent depth enhancement filters intended for Time-of-Flight cameras, and adapt them to alternative depth sensing modalities, both active using an RGB-D camera and passive using a dense stereo camera. To that end, we propose …
Polyethylenimine is a strong inhibitor of human papillomavirus and cytomegalovirus infection.
2012
ABSTRACT Polyethylenimines are cationic polymers with potential as delivery vectors in gene therapy and with proven antimicrobial activity. However, the antiviral activity of polyethylenimines has not been addressed in detail thus far. We have studied the inhibitory effects of a linear 25-kDa polyethylenimine on infections with human papillomaviruses and human cytomegaloviruses. Preincubation of cells with polyethylenimine blocked primary attachment of both viruses to cells, resulting in a significant reduction of infection. In addition, the dissemination of human cytomegalovirus in culture cells was efficiently reduced by recurrent administration of polyethylenimine. Polyethylenimine conce…