Search results for " envelope"
showing 10 items of 253 documents
Evaluation and application of Opuntia ficus-indica insulation panel
2015
Today the attention to the building sustainability issue is increasingly important, because of the major responsibility of the buildings field in the environmental degradation: both in terms of energy consumption from non-renewable sources and the raw materials impoverishment. In addition, the level of comfort required by users are growing and the thermal insulation of buildings becomes the key element in the reduction of energy consumption and C02 emissions, both in the case of new construction and existing ones. The thermal insulation of buildings is the key element in the reduction of energy consumption and C02 emissions due to the cooling of building interiors, both in the case of new c…
Combined Therapy of Interferon Plus Ribavirin Promotes Multiple Adaptive Solutions in Hepatitis C Virus
2009
Hepatitis C virus (HCV) presents several regions involved potentially in evading antiviral treatment and host immune system. Two regions, known as PKR-BD and V3 domains, have been proposed to be involved in resistance to interferon. Additionally, hypervariable regions in the envelope E2 glycoprotein are also good candidates to participate in evasion from the immune system. In this study, we have used a cohort of 22 non-responder patients to combined therapy (interferon alpha-2a plus ribavirin) for which samples obtained just before initiation of therapy and after 6 or/and 12 months of treatment were available. A range of 25-100 clones per patient, genome region and time sample were obtained…
The human peroxisome in health and disease: The story of an oddity becoming a vital organelle
2013
Abstract Since the first report by Rhodin in 1954, our knowledge on mammalian microbodies/peroxisomes has known several periods. An initial two decades period (1954–1973) has contributed to the biochemical individualisation of peroxisomes as a new class of subcellular organelles (de Duve, 1965). The corresponding research period failed to define a clear role of mammalian peroxisomes in vital functions and intermediary metabolism, explaining why feeling that peroxisomes might be in the human cell oddities has prevailed during several decades. The period standing from 1973 to nowadays has progressively removed this cell oddity view of peroxisomes by highlighting vital function and metabolic r…
Enhanced baculovirus-mediated transduction of human cancer cells by tumor-homing peptides.
2006
ABSTRACT Tumor cells and vasculature offer specific targets for the selective delivery of therapeutic genes. To achieve tumor-specific gene transfer, baculovirus tropism was manipulated by viral envelope modification using baculovirus display technology. LyP-1, F3, and CGKRK tumor-homing peptides, originally identified by in vivo screening of phage display libraries, were fused to the transmembrane anchor of vesicular stomatitis virus G protein and displayed on the baculoviral surface. The fusion proteins were successfully incorporated into budded virions, which showed two- to fivefold-improved binding to human breast carcinoma (MDA-MB-435) and hepatocarcinoma (HepG2) cells. The LyP-1 pepti…
Baculovirus Display: A Multifunctional Technology for Gene Delivery and Eukaryotic Library Development
2006
For over a decade, phage display has proven to be of immense value, allowing selection of a large variety of genes with novel functions from diverse libraries. However, the folding and modification requirements of complex proteins place a severe constraint on the type of protein that can be successfully displayed using this strategy, a restriction that could be resolved by similarly engineering a eukaryotic virus for display purposes. The quite recently established eukaryotic molecular biology tool, the baculovirus display vector system (BDVS), allows combination of genotype with phenotype and thereby enables presentation of eukaryotic proteins on the viral envelope or capsid. Data have sho…
Inactivation of an Enveloped Virus by Immobilized Antimicrobial Peptides.
2021
Infections caused by viruses are difficult to treat due to their life cycle, which depends on the replication machinery of the respective host cells. Commonly used antiviral strategies are based upon the application of, e.g., entry inhibitors and other compounds that interfere with virus replication. Besides possible side effects, the rapid occurrence of viral resistance poses a great challenge. Antimicrobial peptides (AMPs), as a component of the innate immunity, are able to kill bacteria and fungi and, in addition, may inactivate enveloped viruses. Many AMPs exert their biological function by impairing microbial and viral membranes. As a result, membrane integrity is lost, leading to bact…
Properties of baculovirus particles displaying GFP analyzed by fluorescence correlation spectroscopy.
2003
Abstract Recombinant baculovirus particles displaying green fluorescent protein (GFP) fused to the major envelope glycoprotein gp64 of the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) were characterized by fluorescence correlation spectroscopy (FCS). FCS detected Brownian motion of single, intact recombinant baculovirus display particles with a diffusion coefficient (D) of (2.89±0.74)10 8 cm2s 1 and an apparent hydrodynamic radius of 83.35±21.22 nm. In the presence of sodium dodecyl sulfate (SDS), Triton X-100, and octylglucoside, the diffusion time was reduced to the 0.2 ms range (D = 7.5710 7 cm2s 1), showing that the fusion proteins were anchored in the viral envelope…
Be stars in open clusters. I. uvby $ ^{\bf \beta}$ photometry
1996
We present uvby β photometry for Be stars in eight open clusters and two OB associations. It is shown that Be stars occupy anomalous positions in the photometric diagrams, which can be explained in terms of the circumstellar continuum radiation contribution to the photometric indices. In the - M V plane Be stars appear redder than the non emission B stars, due to the additional reddening caused by the hydrogen free-bound and free-free recombination in the circumstellar envelope. In the c 0 - M V plane the earlier Be stars present lower c 0 values than absorption-line B stars, which is caused by emission in the Balmer discontinuity, while the later Be stars deviate towards higher c 0 values,…
Simultaneous uvbyβ Photometry and Hα Spectroscopy of Be Stars in Open Clusters
1994
The usual methods of spectral clasification, equivalent widths of Balmer lines or photometric calibrations are not suitable for the determination of the astrophysical parameters of the underlying star in Be-type objects. The spectrum is distorted by the circumstellar envelope lines, while the contribution of the envelope continuum radiation contaminates the photometric indices.
The unusual γ-ray burst GRB 101225A from a helium star/neutron star merger at redshift 0.33
2011
Long Gamma-Ray Bursts (GRBs) are the most dramatic examples of massive stellar deaths, usually associated with supernovae. They release ultra-relativistic jets producing non-thermal emission through synchrotron radiation as they interact with the surrounding medium. Here we report observations of the peculiar GRB 101225A (the "Christmas burst"). Its gamma-ray emission was exceptionally long and followed by a bright X-ray transient with a hot thermal component and an unusual optical couuterpart. During the first 10 days, the optical emission evolved as an expanding, cooling blackbody after which an additional component, consistent with a faint supernova, emerged. We determine its distance to…