Search results for " expression"
showing 10 items of 4731 documents
Heterogeneity within and between primary colorectal carcinomas and matched metastases as revealed by analysis of Ki-ras and p53 mutations
2004
Analysis of the genetic status of Ki-ras and p53 in primary colorectal carcinomas and matched colorectal liver metastasis from 30 patients reveals an overall heterogeneity both within and between the two tumoral tissues. Both genes were found mutated with a similar frequency in both tissues; however, identical mutations in primary tumor and matched metastasis were found less frequently in the case of the Ki-ras than the p53 gene. Only in three cases the same p53 and Ki-ras mutations found in the primary tumor were found also in the metastasis. In several metastatic specimens the DNA bearing a mutation detected also in the primary tumor appears significantly less abundant than the wild-type …
UV-B exposure causes DNA damage and changes in protein expression in northern pike (Esox lucius) posthatched embryos.
2012
The ongoing anthropogenically caused ozone depletion and climate change has increased the amount of biologically harmful UV-B radiation, which is detrimental to fish in embryonal stages. The effects of UV-B radiation on the levels and locations of DNA damage manifested as cyclobutane pyrimidine dimers (CPDs), heat shock protein 70 (HSP70) and p53 protein in newly hatched embryos of pike were examined. Pike larvae were exposed in the laboratory to current and enhanced doses of UV-B radiation. UV-B exposure caused the formation of CPDs in a fluence rate-dependent manner, and the CPDs were found deeper in the tissues with increasing fluence rates. UV-B radiation induced HSP70 in epidermis, and…
Targeting HSP90 with the small molecule inhibitor AUY922 (luminespib) as a treatment strategy against hepatocellular carcinoma
2018
Hepatocellular carcinoma (HCC) is a highly malignant tumor that responds very poorly to existing therapies, most probably due to its extraordinary inter- and intra-tumor molecular heterogeneity. The modest therapeutic response to molecular targeted agents underlines the need for new therapeutic approaches for HCC. In our study, we took advantage of well-characterized human HCC cell lines, differing in transcriptomic subtypes, DNA mutation and amplification alterations, reflecting the heterogeneity of primary HCCs, to provide a preclinical evaluation of the specific heat shock protein 90 (HSP90) inhibitor AUY922 (luminespib). Indeed, HSP90 is highly expressed in different tumor types, but it…
pD-l1 expression in oral squamous cell carcinoma microenvironment and prognostic correlations
2018
Backgroun of this study is to investigate the PD-l1 expression in the Microenvironment of oral Squamous cell carcinoma (oScc) samples and its correlation to prognostic data.
Nuorten miesten kirjeitse ohjailemat seurustelusuhteet 1800-luvun lopun sisäsuomalaisella maaseudulla
2014
Artikkelissa tarkastellaan 1800-luvun Keuruun käräjäkunnassa, sisäsuomalaisella maaseudulla eläneiden nuorten miesten ja naisten seurustelusuhteiden kehittymistä kohti avioliittoa ja sitä, kuinka tälle tielle alkoi kasautua ongelmia. Tutkimuksen ensisijaisena lähdeaineistona ovat miesten seurustelusuhteiden aikana kumppaneilleen lähettämät intiimit yksityiskirjeet (12 kappaletta), jotka naiset olivat tuoneet aviottoman lapsen elatusoikeudenkäynteihin todisteeksi suhteen aikaisesta seksuaalisesta kanssakäymisestä. Artikkeli sijoittuu kansanihmisten kirjoittamia tekstejä tarkastelevaan monitieteiseen historiantutkimuksen alaan. Artikkelissa esitetään, että kirjeet kirjoittaneet miehet pyrkivä…
Intergenerational Effect of Early Life Exposure to Permethrin: Changes in Global DNA Methylation and in Nurr1 Gene Expression
2015
Environmental exposure to pesticides during the early stages of development represents an important risk factor for the onset of neurodegenerative diseases in adult age. Neonatal exposure to Permethrin (PERM), a member of the family of synthetic pyrethroids, can induce a Parkinson-like disease and cause some alterations in striatum of rats, involving both genetic and epigenetic pathways. Through gene expression analysis and global DNA methylation assessment in both PERM-treated parents and their untreated offspring, we investigated on the prospective intergenerational effect of this pesticide. Thirty-three percent of progeny presents the same Nurr1 alteration as rats exposed to permethrin i…
Induction of body weight loss through RNAi-knockdown of APOBEC1 gene expression in transgenic rabbits
2014
In the search of new strategies to fight against obesity, we targeted a gene pathway involved in energy uptake. We have thus investigated the APOB mRNA editing protein (APOBEC1) gene pathway that is involved in fat absorption in the intestine. The APOB gene encodes two proteins, APOB100 and APOB48, via the editing of a single nucleotide in the APOB mRNA by the APOBEC1 enzyme. The APOB48 protein is mandatory for the synthesis of chylomicrons by intestinal cells to transport dietary lipids and cholesterol. We produced transgenic rabbits expressing permanently and ubiquitously a small hairpin RNA targeting the rabbit APOBEC1 mRNA. These rabbits exhibited a moderately but significantly reduced …
Characterization and transcription studies of a phytochelatin synthase gene from the solitary tunicate Ciona intestinalis exposed to cadmium.
2014
The major thiol-containing molecules involved in controlling the level of intracellular ROS in eukaryotes, acting as a nonenzymatic detoxification system, are metallothioneins (MTs), glutathione (GSH) and phytochelatins (PCs). Both MTs and GSH are well-known in the animal kingdom. PC was considered a prerogative of the plant kingdom but, in 2001, a phytochelatin synthase (PCS) gene was described in the nematode Caenorhabditis elegans; additional genes encoding this enzyme were later described in the earthworm Eisenia fetida and in the parasitic nematode Schistosoma mansoni but scanty data are available, up to now, for Deuterostomes. Here, we describe the molecular characteristics and transc…
Soil inoculation with symbiotic microorganisms promotes plant growth and nutrient transporter genes expression in durum wheat
2015
In a field experiment conducted in a Mediterranean area of inner Sicily, durum wheat was inoculated with plant growth-promoting rhizobacteria (PGPR), with arbuscular mycorrhizal fungi (AMF), or with both to evaluate their effects on nutrient uptake, plant growth, and the expression of key transporter genes involved in nitrogen (N) and phosphorus (P) uptake. These biotic associations were studied under either low N availability (unfertilized plots) and supplying the soil with an easily mineralizable organic fertilizer. Regardless of N fertilization, at the tillering stage, inoculation with AMF alone or in combination with PGPR increased the aboveground biomass yield compared to the uninocula…
Tiesību uz vārda brīvību mijiedarbība ar pašvaldību tiesībām izdot pašvaldību plašsaziņas līdzekli
2018
Mūsdienās pašvaldību plašsaziņas līdzekļi satur ne tikai ar pašvaldības domes darbu saistītu informāciju, bet arī komerciālas reklāmas un sludinājumus, kas ir galvenais ienākumu gūšanas avots privāto plašsaziņas līdzekļu īpašniekiem. Šī iemesla dēļ sabiedrībā pastāv diskusijas par to, vai pašvaldību plašsaziņas līdzekļu izdošana nepārkāpj tiesības uz vārda brīvību, neapdraud privāto plašsaziņas līdzekļu pastāvēšanu mediju tirgū un nav pretrunā godīgai konkurencei. Par pašvaldību plašsaziņas līdzekļu lomu un saturu publiskajā telpā ir pausti dažādi viedokļi. Lai gan ir saprotams privāto plašsaziņas līdzekļu īpašnieku viedoklis par to, ka pašvaldību plašsaziņas līdzekļu izdevējiem nebūtu jāie…