Search results for " fingerprinting"

Intra-cultivar Diversity in sicilian and calabrian olive (olea europaea L.) Cultivars Depicted by Morphological Traits and SSR Markers

2012

In this study, SSR analysis was performed on a large number of putative clones of the main olive cultivars from two Italian regions, Sicily and Calabria, in order to study the genetic relatedness, and to detect any degree of genetic diversity, which can be used to unambiguously identify possible superior clones. These putative clones have been collected and observed in the last ten years, following an extensive investigation carried out in traditional olive growing areas, thanks to the reports of farmers, millers, technician, growers and nurseries. The set of microsatellite (SSR) markers used allowed the detection of the genetic diversity, resulting from somatic mutation, indicating the pre…

Chemical, biochemical and microbial diversity through a Pachic Humudept profile in a temperate upland grassland

2013

There is great interest in understanding the factors that drive soil microbial activity and community composition in upland grassland ecosystems. We investigated the role of vertical gradients of chemical properties and various soluble C and N pools on soil microbial community structure by using a combination of chemical and biochemical methods coupled with PCR-denaturing gradient gel electrophoresis (DGGE) community fingerprinting and phospholipid fatty acid (PLFA) profiling. Soil samples were collected at increasing depth from a temperate upland grassland. Soil organic matter-related pools (total organic C and total Kjeldahl N) and functionally related active pools (microbial biomass C an…

INDOOR LOCALIZATION USING COGNITIVE RADIOS AND SOFTWARE-DEFINED WIRELESS NETWORKS

THE FINGERPRINT OF THE HUMAN GASTROINTESTINAL TRACT MICROBIOTA: A HYPOTHESIS OF MOLECULAR MAPPING

2017

The precise etiology of Inflammatory Bowel Disease (IDB) remains unclear and several factors are believed to play a role in its development and progression, including the composition of microbial communities resident in the gastrointestinal tract. Human intestinal microbiota are extensive with at least 15,000-36,000 bacterial species. However, thanks to the new development in sequencing and molecular taxonomic methodologies, our understanding of the microbiota population composition, dynamics, and ecology has greatly increased. Intestinal microbiota play a critical role in the maintenance of the host intestinal barrier homeostasis, while dysbiosis, which involves reduction in the microbiome…

Application of DNA techniques for identification using human dental pulp as a source of DNA

1992

Dental pulp tissue could be obtained in most cases from materials obtained under experimental conditions and from forensic casework (air accidents, burned and putrefied bodies). Teeth extracted during dental treatment (n = 30) were stored for 6 weeks and 4 years at room temperature. In addition teeth (n = 10) extracted from jaw fragments that had been stored for 15 years at room temperature, and teeth extracted post mortem from actual identification cases (n = 8) were investigated. Following extraction from dental pulp tissue the DNA concentration was measured by fluorometry. The amount of DNA obtained from the dental pulp tissue of a single tooth varied from 6 micrograms to 50 micrograms D…

The dynamics of soil bacterial community structure in response to yearly repeated agricultural copper treatments

2008

International audience; The annual dynamics of soil bacterial community structure, including early, dose-dependent and transient modifications, was observed consecutively at different levels of copper contamination (high: 48 kg Cu ha−1, low: 16 kg Cu ha−1) repeated yearly over a three-year field experiment. Repeated low-level Cu contamination led to an increase in community stability to metal stress without a long-term shift in the population structure, whereas repeated high-level Cu contamination induced a novel and stable bacterial community structure. Furthermore, field experimentation highlighted that episodic climatic stress can modulate copper impact by enhancing community stability.

Protein and DNA fingerprinting of a soil bacterial community inoculated into three different sterile soils

2007

The functional and genetic structures of a soil bacterial community were characterized after inoculation into three different sterile soils using a protein and DNA fingerprinting method, respectively. Principal component analysis (PCA) of profiles revealed that, depending on soil characteristics, bacterial communities with similar genetic structures harbored different functional structures and thus could potentially be of differing ecological significance for soil functioning. Co-inertia analysis between protein fingerprinting data and the corresponding sets of soil physicochemical characteristics demonstrated the correlation between the functional structure of the bacterial community and s…

Sampling strategy in molecular microbial ecology: influence of soil sample size on DNA fingerprinting analysis of fungal and bacterial communities.

2003

Assessing soil microbial community structure by the use of molecular techniques requires a satisfactory sampling strategy that takes into account the high microbial diversity and the heterogeneous distribution of microorganisms in the soil matrix. The influence of the sample size of three different soil types (sand, silt and clay soils) on the DNA yield and analysis of bacterial and fungal community structure were investigated. Six sample sizes from 0.125 g to 4 g were evaluated. The genetic community structure was assessed by automated ribosomal intergenic spacer analysis (A-RISA fingerprint). Variations between bacterial (B-ARISA) and fungal (F-ARISA) community structure were quantified b…

Soil REE patterns as tracers of the emplacement of metal-rich anthropogenic materials. A case study in Moa (Cuba)

2019

Purpose: Are rare earth element (REE) patterns employable as tracers in human interventions of bulk soil disturbances, when high concentrations of metals are involved in the in situ soil disturbance? In terms of bulk soil disturbance, it is difficult to distinguish between bulk soil disturbances and emplacement of new earthen material. Chemical fingerprinting, in particular REE plus yttrium, is applied across many fields, but predominantlyin tracking the sources of potential environmental contaminants. Materials and methods: The REE normalised patterns (HNO 3 :HF:HCl 3:1:1 digestion, ICP-MS) of two Calcaric Cambisols were compared with those of a Geric Ferralsol (Novic, Technic) originated …

Wickerhamomyces sylviae f.a., sp. nov., an ascomycetous yeast species isolated from migratory birds.

2013

In the present work, we investigated the phylogenetic position and phenotypic characteristics of eight yeast isolates collected from migratory birds on the island of Ustica, Italy. A phylogenetic analysis based on the D1/D2 region of the large-subunit rRNA gene showed that all isolates clustered as a single separate lineage within the Wickerhamomyces clade. They exhibited distinct morphological and physiological characteristics and were clearly separated from their closest relatives, Wickerhamomyces lynferdii, Wickerhamomyces anomalus and Wickerhamomyces subpelliculosus, in blastn searches. On the basis of the isolation source, physiological features and molecular strain typing carried out …