Search results for " fusion"

Constitutive expression of clathrin hub hinders elicitor-induced clathrin-mediated endocytosis and defense gene expression in plant cells.

2012

International audience; Endocytosis has been recently implicated in the signaling network associated with the recognition of microbes by plants. In a previous study, we showed that the elicitor cryptogein was able to induce clathrin-mediated endocytosis (CME) in tobacco suspension cells. Herein, we investigate further the induced CME by means of a GFP-tagged clathrin light chain and a CME inhibitor, the hub domain of clathrin heavy chain. Hub constitutive expression does affect neither cell growth nor constitutive endocytosis but abolishes cryptogein-induced CME. Such an inhibition has no impact on early events in the cryptogein signaling pathway but reduces the expression of defense-associ…

In vivoanalysis of the lumenal binding protein (BiP) reveals multiple functions of its ATPase domain

2007

International audience; The endoplasmic reticulum (ER) chaperone binding protein (BiP) binds exposed hydrophobic regions of misfolded proteins. Cycles of ATP hydrolysis and nucleotide exchange on the ATPase domain were shown to regulate the function of the ligand-binding domain in vitro. Here we show that ATPase mutants of BiP with defective ATP-hydrolysis (T46G) or ATP-binding (G235D) caused permanent association with a model ligand, but also interfered with the production of secretory, but not cytosolic, proteins in vivo. Furthermore, the negative effect of BiP(T46G) on secretory protein synthesis was rescued by increased levels of wild-type BiP, whereas the G235D mutation was dominant. U…

Cost-Effective eHealth System Based on a Multi-Sensor System-on-Chip Platform and Data Fusion in Cloud for Sport Activity Monitoring

2018

eHealth systems provide medical support to users and contribute to the development of mobile and quality health care. They also provide results on the prevention and follow-up of diseases by monitoring health-status indicators and methodical data gathering in patients. Telematic management of health services by means of the Internet of Things provides immediate support and it is cheaper than conventional physical presence methods. Currently, wireless communications and sensor networks allow a person or group to be monitored remotely. The aim of this paper is to develop and assess a system for monitoring physiological parameters to be applied in different scenarios, such as health or sports.…

Integrated GNSS/IMU Hub Motion Estimator for Offshore Wind Turbine Blade Installation

2019

Abstract Offshore wind turbines (OWTs) have become increasingly popular for their ability to harvest clean offshore wind energy. Bottom-fixed foundations are the most used foundation type. Because of its large diameter, the foundation is sensitive to wave loads. For typical manually assisted blade-mating operations, the decision to perform the mating operation is based on the relative distance and velocity between the blade root center and the hub, and in accordance with the weather window. Hence, monitoring the hub real-time position and velocity is necessary, whether the blade installation is conducted manually or automatically. In this study, we design a hub motion estimation algorithm f…

Ship-to-Ship State Observer Using Sensor Fusion and the Extended Kalman Filter

2019

In this paper, a solution for estimating the relative position and orientation between two ships in six degrees-of-freedom (6DOF) using sensor fusion and an extended Kalman filter (EKF) approach is presented. Two different sensor types, based on time-of-flight and inertial measurement principles, were combined to create a reliable and redundant estimate of the relative motion between the ships. An accurate and reliable relative motion estimate is expected to be a key enabler for future ship-to-ship operations, such as autonomous load transfer and handling. The proposed sensor fusion algorithm was tested with real sensors (two motion reference units (MRS) and a laser tracker) and an experime…

An adaptive multi-rate system for visual tracking in augmented reality applications

2016

The visual tracking of an object is a well-known problem, and it involves many fields of applications. Often a single sensor, the camera, could not provide enough information in order to track the whole object trajectory due to a low updating rate; therefore a multi-sensor system, based also on inertial measurements, could be necessary to improve the tracking accuracy. This leads to the fundamental question: how can information from different sensors be combined when they work at different rates? In this paper an approach based on recursive parameter estimation focusing on multi-rate situations is suggested. The problem is here formulated as the state-of-the-art problem of the visual tracki…

Detection of RET rearrangements in papillary thyroid carcinoma using RT-PCR and FISH techniques - A molecular and clinical analysis.

2019

Abstract Introduction Oncogenic BRAF and RAS mutations as well as multiple known (and yet unknown) RET fusion oncogenes comprise the majority of causative molecular alterations in papillary thyroid carcinoma (PTC). Apparently “mutation-negative” PTCs encompass a heterogenous group impeding analysis of prognostic significance of underlying genetics. Material and methods BRAF wild type PTC tissue of 56 patients was analyzed using two established methods: hybrid-specific RT-PCR for the predominant rearrangement RET/PTC1 and fluorescent in situ hybridization (FISH). Clinical features of the cases with and without RET rearrangement were compared (patient age, gender, tumor size, focality, lymph …

Are Mitochondrial Fusion and Fission Impaired in Leukocytes of Type 2 Diabetic Patients?

2016

Mitochondrial fusion/fission alterations have been evaluated in different tissues of type 2 diabetic (T2D) patients. However, it is not known whether mitochondrial dynamics is disturbed in the leukocytes of T2D patients and whether glycemic control affects its regulation. Anthropometric and metabolic parameters in 91 T2D patients (48 with glycated hemoglobin [HbA1c]6.5% and 43 with HbA1c6.5%) were characteristic of the disease when compared with 78 control subjects. We observed increased reactive oxygen species production in leukocytes from diabetic patients, together with a reduced mitochondrial oxygen consumption rate, especially in poorly controlled patients. Mitochondrial fusion was red…

Annulate Lamellae in a Desmoplastic Small Round Cell Tumor

2016

Solitary Fibrous Tumor of the Vulva: Report of 2 Cases, Including a De Novo Dedifferentiated Solitary Fibrous Tumor Diagnosed After Molecular Demonst…

2018

Solitary fibrous tumor (SFT) is a neoplasm of fibroblastic lineage that has been documented in almost every anatomic location. Vulval SFT is very rare with only 10 cases reported to date. We present 2 additional SFTs located in the vulva, in adult women of 59 and 25 yr of age. The first showed a classic morphology and immunophenotype with uniform and strong STAT6 nuclear expression. The other one was a spindle-cell de novo dedifferentiated SFT with heterogeneous nuclear and cytoplasmic STAT6 staining, which could only be correctly diagnosed after molecular analysis with demonstration of a NAB2-STAT6 gene fusion. This genetic aberration is considered to represent the major pathogenic driver …