Search results for " isola"

FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/β-catenin signaling pathway reporter

2021

Summary Cancer stem cells (CSCs) play a key role in tumor initiation and progression. A real-time tool to evaluate the activation of CSC-specific signaling pathways is crucial for the study of this cancer cell subset. Here, we present a protocol to monitor, in vitro, the activation of Wnt/β-catenin signaling pathway, which is considered a functional biomarker for colorectal CSCs (CR-CSCs). This flow-cytometry-based protocol allows it to isolate CR-CSCs and to evaluate their cytotoxicity upon anti-tumor treatments. For complete details on the use and execution of this protocol, please refer to Di Franco et al. (2021).

Effectiveness of isolated vertical extension of masonry buildings as nonconventional TMD

2023

In this paper, the strategy of Intermediate Isolation System (IIS) is proposed for the vertical extension of an aggregate of masonry buildings located in Pozzuoli, south Italy. The effectiveness of IIS working as a nonconventional Tuned Mass Damper (TMD) for the existing construction is assessed in terms of reduction of seismic demand and damage in the masonry structure undergoing inelastic deformation under seismic input. For this aim, a general step-by-step procedure is first defined and then applied to the case study building. In particular, the characterization of dynamic behaviour and seismic capacity of the building aggregate is first addressed through modal and push over analyses. El…

Pheno-genotyping of Salmonella enterica serotype Enteritidis isolates identified in Sicily during a reemergence period.

2005

After an upward trend paralleling that occurring in most European countries, including Italy, since October 2002 Salmonella enterica serotype Enteritidis (S. Enteritidis) has again gained the first position among outbreak and sporadic human isolates of Salmonella in Sicily. Because phage typing of S. Enteritidis has many technical and epidemiological limitations and molecular methods have proved to be poorly discriminative for this organism, multiple typing, using phage typing together with pulsed field gel electrophoresis (PFGE) and plasmid profiling on a sample of fifty human and poultry isolates identified during the period October 2002 to May 2003 in Sicily, was chosen as the most valua…

Parameter Identification of a Winding Function Based Model for Fault Detection of Induction Machines

2018

Prediction of machines' faulty parts is important in industrial applications in order to reduce productivity losses. As far as electrical machines are considered, a model-based fault diagnosis approach is usually used for this purpose. The model is derived from the modified winding function theory and hence, it requires a considerable amount of parameters at various operating conditions in order to be successfully used. However, the complete set of parameters is difficult to be obtained, as manufacturers of electric machines normally provide only the parameters that describe simple motor models (e.g. T-equivalent circuit at rated conditions). Therefore, the current work presents a method th…

Advancement on isolated microspore culture in Citrus clementina Hort. ex Tan., cvs. Monreal Rosso and Nules.

2013

Coltura in vitro di microspore isolate per il miglioramento genetico di Olea europaea L.

2013

Gli aploidi (piante con un numero gametofitico di cromosomi nello sporofito: n invece di 2n) ed i doppio-aploidi (aploidi che hanno raddoppiato il numero di cromosomi spontaneamente o in maniera indotta) trovano numerose applicazioni nel miglioramento genetico vegetale, ed in particolare, nella mutagenesi, nella selezione in vitro, nella trasformazione, nell’utilizzo della variabilità gametoclonale ed anche nel sequenziamento del genoma e negli studi di genomica funzionale. Pertanto, essi costituiscono uno strumento biotecnologico particolarmente interessante nel miglioramento genetico delle piante. Ai fini dell’ottenimento dell’omozigosi nell’olivo, come in tutte le piante arboree, caratte…

Study on isolated microspore culture in Citrus sinensis Osbeck cv. Moro, a blood orange cultivar].

2014

Indagini preliminari di crioconservazione in embrioni isolati di Abies nebrodensis (Lojac.) Mattei

2009

Dematiaceous Hyphomycetes from Bosco Isola (S Italy) Mediterranean maquis litter

2010

This is the fifth contribution to the knowledge of Dematiaceous Hyphomycetes colonizing the litter in Mediterranean maquis. Twelve species are described and notes on the morphological characters are pointed out. Three of them are reported as genus, they need an examination of more material

Seagrass meadows at the extreme of environmental tolerance: the case ofPosidonia oceanicain a semi-enclosed coastal lagoon

2009

Atoll-like structures of the endemic Mediterranean seagrass Posidonia oceanica were encountered in the innermost area of the Stagnone di Marsala, a semi-enclosed coastal lagoon along the western coasts of Sicily. The area is characterized by limited water exchange with the open sea and by a marked seasonal variation of water salinity and temperature, reaching beyond the theoretical tolerance limits of the species. In the present study we determined the genetic composition of the atoll-like structures, as well as the growth performance and flowering rate of these stands. We also assessed whether and to what extent the atoll-like structures are genetically isolated from plants growing in mead…