Search results for " laboratori"

showing 10 items of 167 documents

Betulinic Acid Kills Colon Cancer Stem Cells

2016

Cancer stem cells (CSCs) are considered to be the origin of cancer and it is suggested that they are resistant to chemotherapy. Current therapies fail to eradicate CSCs and therefore selecting a resistant cell subset that is able to facilitate tumor recurrences. Betulinic acid (BetA) is a broad acting natural compound, shown to induce cell death via the inhibition of the stearoyl-CoA- desaturase (SCD- 1). This enzyme converts saturated fatty acids into unsaturated fatty acids and is over-expressed in tumor cells. Here we show that BetA induces rapid cell death in all colon CSCs tested and is able to affect the CSCs directly as shown, via the loss of clonogenic capacity. Similar results were…

0301 basic medicineProgrammed cell deathColorectal cancerMedicine (miscellaneous)Biology03 medical and health scienceschemistry.chemical_compound0302 clinical medicineCancer stem cellBetulinic acidCell Line TumormedicineHumansEnzyme InhibitorsClonogenic assayCell DeathCancer stem cellStearoyl CoA-desaturaseCancerGeneral Medicinemedicine.diseaseBetulinic acidTriterpenesClone CellsColon cancerTumor resistance030104 developmental biologychemistryBiochemistryCell culture030220 oncology & carcinogenesisCancer treatmentColonic NeoplasmsMutationCancer researchNeoplastic Stem CellsStem cellSettore MED/46 - Scienze Tecniche Di Medicina Di LaboratorioPentacyclic TriterpenesStearoyl-CoA DesaturaseCurrent stem cell research & therapy
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Cancer stem cell-based models of colorectal cancer reveal molecular determinants of therapy resistance

2016

Abstract Colorectal cancer (CRC) therapy mainly relies on the use of conventional chemotherapeutic drugs combined, in a subset of patients, with epidermal growth factor receptor [EGFR]-targeting agents. Although CRC is considered a prototype of a cancer stem cell (CSC)-driven tumor, the effects of both conventional and targeted therapies on the CSC compartment are largely unknown. We have optimized a protocol for colorectal CSC isolation that allowed us to obtain CSC-enriched cultures from primary tumor specimens, with high efficiency. CSC isolation was followed by in vitro and in vivo validation, genetic characterization, and drug sensitivity analysis, thus generating panels of CSC lines w…

0301 basic medicineProteomicscancer stem cellsColorectal cancerDrug ResistanceMice SCIDAnti-EGFR therapy; Cancer stem cells; Cetuximab; Colorectal cancer; Proteomic arrays; Animals; Cells Cultured; Colorectal Neoplasms; Drug Resistance Neoplasm; Female; Gene Expression Profiling; Humans; Mice Inbred NOD; Mice SCID; Mice Transgenic; Microarray Analysis; Models Biological; Neoplastic Stem Cells; Protein Kinase Inhibitors; Proteomics; Signal Transduction; Developmental Biology; Cell BiologyTransgenicMiceMice Inbred NODModelsproteomic arrayscetuximabcell biologyEpidermal growth factor receptorCells CulturedCulturedCetuximabbiologyGeneral MedicinePrimary tumorNeoplastic Stem CellsFemaleSettore MED/46 - Scienze Tecniche Di Medicina Di LaboratorioStem cellColorectal Neoplasmsmedicine.drugSignal TransductionCellsMice Transgeniccolorectal cancerSCIDModels Biological03 medical and health sciencesdevelopmental biologyProteomic arrayCancer stem cellIn vivoSettore MED/04 - PATOLOGIA GENERALEmedicineAnimalsHumansProtein Kinase InhibitorsSettore MED/06 - ONCOLOGIA MEDICAMicroarray analysis techniquesbusiness.industryCancer stem cellGene Expression Profilingmedicine.diseaseMicroarray AnalysisBiological030104 developmental biologyanti-EGFR therapyDrug Resistance Neoplasmanti-EGFR therapy; cancer stem cells; cetuximab; colorectal cancer; proteomic arrays; cell biology; developmental biologyImmunologyCancer researchbiology.proteinNeoplasmInbred NODbusiness
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ROS and Lipid Droplet accumulation induced by high glucose exposure in healthy colon and Colorectal Cancer Stem Cells

2020

Lipid Droplets (LDs) are emerging as crucial players in colon cancer development and maintenance. Their expression has been associated with high tumorigenicity in Cancer Stem Cells (CSCs), so that they have been proposed as a new functional marker in Colorectal Cancer Stem Cells (CR-CSCs). They are also indirectly involved in the modulation of the tumor microenvironment through the production of pro-inflammatory molecules. There is growing evidence that a possible connection between metabolic alterations and malignant transformation exists, although the effects of nutrients, primarily glucose, on the CSC behavior are still mostly unexplored. Glucose is an essential fuel for cancer cells, an…

0301 basic medicinelcsh:QH426-470Colorectal cancerColorectal cancer stem cellsSettore MED/50 - Scienze Tecniche Mediche ApplicateLipid dropletBiochemistryMalignant transformation03 medical and health sciences0302 clinical medicineCancer stem cellLipid dropletFull Length ArticlemedicineCholesterol metabolismMolecular BiologyOncogeneGenetics (clinical)PI3K/AKT/mTOR pathwaySettore MED/04 - Patologia Generalelcsh:R5-920Tumor microenvironmentChemistryPI3K-AKTColorectal cancer stem cellCell BiologyOncogenesmedicine.diseaseLipid droplets3. Good healthlcsh:GeneticsSettore BIO/12 - Biochimica Clinica E Biologia Molecolare Clinica030104 developmental biologyOxidative stress030220 oncology & carcinogenesisFatty acid metabolismCancer cellCancer researchOxidative streSettore MED/46 - Scienze Tecniche Di Medicina Di LaboratorioStem cellHigh glucoselcsh:Medicine (General)Genes & Diseases
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Dietary restriction: could it be considered as speed bump on tumor progression road?

2016

Dietary restrictions, including fasting (or long-term starvation), calorie restriction (CR), and short-term starvation (STS), are considered a strong rationale that may protect against various diseases, including age-related diseases and cancer. Among dietary approaches, STS, in which food is not consumed during designed fasting periods but is typically not restricted during designated feeding periods, seems to be more suitable, because other dietary regimens involving prolonged fasting periods could worsen the health conditions of cancer patients, being they already naturally prone to weight loss. Until now, the limited amount of available data does not point to a single gene, pathway, or …

0301 basic medicinemedicine.medical_specialtySettore MED/06 - Oncologia MedicaCalorie restrictionCancer cellBiologyBioinformaticsCellular stress response03 medical and health sciencesWeight lossNeoplasmsCellular stress responseInternal medicinemedicineAnimalsHumansChemotherapyShort-term starvationSettore MED/49 - Scienze Tecniche Dietetiche ApplicateCaloric RestrictionStarvationCancerCancer cells; Cellular stress response; Chemotherapy; Diet; Fasting; Short-term starvationFastingGeneral Medicinemedicine.diseaseDietSettore MED/18 - Chirurgia Generale030104 developmental biologyEndocrinologyTumor progressionCancer cellSettore MED/46 - Scienze Tecniche Di Medicina Di LaboratorioSignal transductionmedicine.symptomTumor Biology
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The first year of the “open discovery of stem laboratories” (ODL) project

2018

The Open Discovery of STEM Laboratories (ODL) project, co-funded by the European Community Erasmus+ KA2 program for 30 months, starting from November 2015, involves five countries: Spain, Italy, Greece, Estonia and Lithuania. It aims to implement teacher collaboration in creating and using μMOOCs (very short version of MOOCs-Massive Open Online Courses) for encouraging the use of STEM (Science, Technology, Engineering and Mathematics) remote/virtual laboratories into lessons. The challenge of the project is to impact on teaching process and inspire pedagogical innovation and modernization by means of open education resources, teaching/learning tools and best practices provided by European e…

050101 languages & linguisticsHistoryEngineeringbusiness.industrySettore FIS/08 - Didattica E Storia Della Fisica05 social sciences050301 education06 humanities and the artsPhysics Education STEM Laboratories Inquiry-based approachSettore FIS/03 - Fisica Della MateriaSettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)Computer Science ApplicationsEducation0501 psychology and cognitive sciencesbusiness0503 educationJournal of Physics: Conference Series
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Thermomineral waters of Greece: geochemical characterization

2020

75 °C). In terms of pH most results vary from 5.5 to 823 °C) ii) warm (23 40 °C) iii) thermal (40 75 °C) and iv) hyperthermal (&gtfew springs show either very low pH (&lt10) proposing serpentinization processes. Regarding TDS concentrations collected waters can be subdivided into low salinity (up to 1.5 g/L) brackish (up to 20 g/L) and saline (up to 43 g/L). The medium high salinities can be justified by mixing with sea water and/or strong waterrock interaction processes. Isotope composition of O and H ranges from 12.7 to +2.7 ‰ SMOW and from 91 to +12 ‰ SMOW respectively and is generally comprised between the Global Meteoric Water Line and the East Mediterranean Meteoric Water Line. Only few water samples show a positive shift for δ18O possibly related to high temperature waterrock interaction processes. Carbon dioxide (18 997000 μmol/mol) or N2 (1100 989000 μmol/mol) or CH4 (&ltMany geothermal areas of Greece are located in regions affected by Miocene or Quaternary volcanism and in continental basins characterised by elevated heat flow. Moreover the majority of them is found along the coast as well as in islands of the Aegean Sea and thus thermal water is often brackish to saline due to marine intrusion into costal aquifer. In the present study almost 300 thermal and cold mineral water samples were collected along the Hellenic territory with their physicochemical parameters (temperature pH electrical conductivity and Eh) and the amount of bicarbonates (titration with 0.1N HCl) being determined in situ. Additionally gases found either in free or dissolved phase were sampled. Both water and gas samples were analysed at the INGVPa laboratories for major ions (Ion Chromatography) silica (Inductively Coupled Plasma Optical Emission Spectrometry) chemical composition of free and dissolved gases (Gas Chromatography) water isotopes (O and H) and carbon and helium isotopes of free and dissolved gases (Mass Spectrometry). The temperature of the investigated waters ranges from 6.5 to 98°C pH from 1.96 to 11.98 whilst Total Dissolved Solids (TDS) from 0.06 to 43 g/L. Based on the temperature parameter waters can be divided into four groups: i) cold (&lt0.5 913000 μmol/mol) are the prevailing gas species found in the studied sites. The δ13CCO2 values ranged from 20.1 to +8.5 ‰ whilst the isotope ratio of He from 0.21 to 6.71 R/RA.4) suggesting interaction with H2Srich gases or very high pH values (&gtSettore GEO/08 - Geochimica E Vulcanologia
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Methodological Aspects of the Application of the Naka-Rushton Equation to Clinical Electroretinogram

1993

The nonlinear relation between stimulus intensity and response amplitude of the electroretinogram (ERG) scotopic b wave can be described by a curve based on the Naka-Rushton (NR) equation. Up to now, the NR equation has been used to assess the features of the normal and pathological ERG, but the best approach for a correct evaluation of the parameters is still debatable. The parameters are thought to be related to the different conditions of retinal activities. The method is well known in experimental laboratories but is quite unusual at the clinical level. In the present paper the derivative analysis of the NR function is proposed as an easier approach to understand the variations of the N…

AdultMaleX ChromosomeAdolescentLightElectrodiagnosisGenetic LinkageVisual AcuityDark AdaptationCataractRetinaCellular and Molecular NeuroscienceExperimental LaboratoriesOpticsRetinal DiseasesElectroretinographymedicineHumansResponse AmplitudeApplied mathematicsScotopic visionChildAgedMathematicsmedicine.diagnostic_testbusiness.industryDerivative analysisGeneral MedicineMiddle AgedSensory SystemsOphthalmologyFemalebusinessRetinitis PigmentosaElectroretinographyOphthalmic Research
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Imparare dall'architettura

2022

Il Laboratorio di progettazione architettonica della prima annualità si rivolge ad allievi che, per quanto non abbiano alcuna esperienza sulla pratica del progetto, sono chiamati, da subito, a confrontarsi con il fare progettuale, e ciò a differenza di quanto, in genere, accade in altri ambiti di apprendimento dell’esercizio di professioni specialistiche come quella dell’architetto. L’incipit, ovvero l’inizio, l’azione dell’incipĕre rappresenta, quindi, l’avvio, il principio, l’incominciare di un percorso formativo in cui tutti, benché neofiti, sono chiamati a un’immediata e iniziale frequenza del “saper fare” riguardo alla progettazione architettonica. E se il divenire dell’architettura, a…

Architettura Laboratorio di Progettazione Architettonica didattica dell'ArchitetturaArchitectureArchitecture teachingSettore ICAR/14 - Composizione Architettonica E UrbanaArchitectural studio
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INCIPIT LAB

2022

Il libro descrive un’esperienza didattica condotta dall’anno accademico 2015/2016 al 2017/2018 e segna l’avvio del coordinamento di Incipit Lab. Tale iniziativa ha offerto un confronto, a livello nazionale e internazionale, fra i laboratori di progettazione architettonica del primo anno accademico. La sperimentazione effettuata trova la sua origine nel coordinamento dei laboratori di primo anno del Corso di Studi in Architettura dell’Università degli Studi di Palermo con il coinvolgimento, inoltre, del Laboratorio di Architettura e Composizione I del Corso di Studi in Ingegneria Edile-Architettura, dello stesso Ateneo. Incipit Lab. propone, attraverso un coordinamento, giunto al suo sesto a…

Architettura Laboratorio di Progettazione Architettonica didattica dell'ArchitetturaArchitectureArchitecture teachingSettore ICAR/14 - Composizione Architettonica E UrbanaArchitectural studio
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Focused Ultrasound Effects on Osteosarcoma Cell Lines

2019

MRI guided Focused Ultrasound (MRgFUS) has shown to be effective therapeutic modality for non-invasive clinical interventions in ablating of uterine fibroids, in bone metastasis palliative treatments, and in breast, liver, and prostate cancer ablation. MRgFUS combines high intensity focused ultrasound (HIFU) with MRI images for treatment planning and real time thermometry monitoring, thus enabling non-invasive ablation of tumor tissue. Although in the literature there are several studies on the Ultrasound (US) effects on cell in culture, there is no systematic evidence of the biological effect of Magnetic Resonance guided Focused Ultrasound Surgery (MRgFUS) treatment on osteosarcoma cells, …

Article SubjectUterine fibroidsSettore MED/06 - Oncologia MedicaCell Survivalmedicine.medical_treatmentlcsh:MedicineBone NeoplasmsThermometryBone NeoplasmGeneral Biochemistry Genetics and Molecular Biology030218 nuclear medicine & medical imaging03 medical and health sciencesProstate cancer0302 clinical medicineSettore BIO/13 - Biologia ApplicataOsteogenesisCell Line TumormedicineHumansOsteosarcomaGeneral Immunology and Microbiologymedicine.diagnostic_testbusiness.industryPhantoms ImagingOsteogenesiUltrasoundlcsh:RBone metastasisMagnetic resonance imagingGeneral Medicinemedicine.diseaseAblationMagnetic Resonance ImagingHigh-intensity focused ultrasound3. Good healthSurgery Computer-Assisted030220 oncology & carcinogenesisOsteosarcomaHigh-Intensity Focused Ultrasound AblationSettore MED/46 - Scienze Tecniche Di Medicina Di LaboratoriobusinessNuclear medicineSettore MED/36 - Diagnostica Per Immagini E RadioterapiaHumanResearch ArticleBioMed Research International
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