Search results for " method"

showing 10 items of 10455 documents

Human Corticotropin-Releasing Hormone in Man: Dose-Response of Minute Ventilation and End-Tidal Partial Pressures of Carbon Dioxide and Oxygen*

1987

The respiratory stimulant properties of iv injections of 33, 67, and 100 micrograms synthetic human corticotropin-releasing hormone (hCRH) were studied in 12 normal men in a single blind, placebo-controlled trial. All doses of hCRH induced a respiratory stimulation in every subject, and the stimulation was dose dependent. The onset of respiratory stimulation occurred within 15-30 sec after hCRH infusion was started. Initially, there was an increase in tidal volume (VT), followed by an increase in respiratory rate. The maximum minute ventilation (VE) occurred 60-120 sec after starting the injection. The 33-micrograms hCRH dose induced a 35% increase in VE from 6.3 +/- 0.6 (+/- SD) to 9.7 +/-…

AdultMalemedicine.medical_specialtyRespiratory rateCorticotropin-Releasing HormonePartial PressureEndocrinology Diabetes and MetabolismClinical BiochemistryBlood PressureStimulationPeptide hormoneBiochemistryEndocrinologyDouble-Blind MethodHeart RateInternal medicineHeart rateFlushingmedicineHumansRespiratory systemTidal volumeDose-Response Relationship DrugChemistryRespirationBiochemistry (medical)Carbon DioxideOxygenEndocrinologyAnesthesiaBreathingRespiratory minute volumeThe Journal of Clinical Endocrinology & Metabolism
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Analysis of thiamine transporter genes in sporadic beriberi

2014

Abstract Objective Thiamine or vitamin B 1 deficiency diminishes thiamine-dependent enzymatic activity, alters mitochondrial function, impairs oxidative metabolism, and causes selective neuronal death. We analyzed for the first time, the role of all known mutations within three specific thiamine carrier genes, SLC19 A2, SLC19 A3 , and SLC25 A19 , in a patient with atrophic beriberi, a multiorgan nutritional disease caused by thiamine deficiency. Methods A 44-year-old male alcoholic patient from Morocco developed massive bilateral leg edema, a subacute sensorimotor neuropathy, and incontinence. Despite normal vitamin B 1 serum levels, his clinical picture was rapidly reverted by high-dose in…

AdultMalemedicine.medical_specialtySLC19 A- SLC25 A19SLC19 AEndocrinology Diabetes and MetabolismGene mutationBeriberimedicine.disease_causeMitochondrial Membrane Transport Proteinslaw.inventionBeriberilawInternal medicineGenotypemedicineThiamine transporterObjective: Thiamine or vitamin B1 deficiency diminishes thiamine-dependent enzymatic activity alters mitochondrial function impairs oxidative metabolism and causes selective neuronal death. We analyzed for the first time the role of all known mutations within three specific thiamine carrier genes SLC19 A2 SLC19 A3 and SLC25 A19 in a patient with atrophic beriberi a multiorgan nutritional disease caused by thiamine deficiency. Methods: A 44-year-old male alcoholic patient from Morocco developed massive bilateral leg edema a subacute sensorimotor neuropathy and incontinence. Despite normal vitamin B1 serum levels his clinical picture was rapidly reverted by high-dose intramuscular thiamine treatment suggesting a possible genetic resistance. We used polymerase chain reaction followed by amplicon sequencing to study all the known thiamine-related gene mutations identified within the Human Gene Mutation Database. Results: Thirty-seven mutations were tested: 29 in SLC19 A2 6 in SLC19 A3 and 2 in SLC25 A19. Mutational analyses showed a wild-type genotype for all sequences investigated. Conclusion: This is the first genetic study in beriberi disease. We did not detect any known mutation in any of the three genes in a sporadic dry beriberi patient. We cannot exclude a role for other known or unknown mutations in the same genes or in other thiamine-associated genes in the occurrence of this nutritional neuropathy.HumansThiamineGenePolymerase chain reactionGeneticsMutationNutrition and DieteticsbiologyMembrane Transport ProteinsThiamine Deficiencymedicine.diseaseAlcoholismEndocrinologyMutationbiology.proteinThiamineMutations
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Oligo-antigenic diet in the treatment of chronic anal fissures. Evidence for a relationship between food hypersensitivity and anal fissures.

2013

Abstract OBJECTIVES: Patients with chronic constipation due to food hypersensitivity (FH) had an elevated anal sphincter resting pressure. No studies have investigated a possible role of FH in anal fissures (AFs). We aimed to evaluate (1) the effectiveness of diet in curing AFs and to evaluate (2) the clinical effects of a double-blind placebo-controlled (DBPC) challenge, using cow's milk protein or wheat. METHODS: One hundred and sixty-one patients with AFs were randomized to receive a "true-elimination diet" or a "sham-elimination diet" for 8 weeks; both groups also received topical nifedipine and lidocaine. Sixty patients who were cured with the "true-elimination diet" underwent DBPC cha…

AdultMalemedicine.medical_specialtySettore MED/09 - Medicina InternaConstipationManometryTreatment outcomeFood habitsSettore MED/08 - Anatomia PatologicaGastroenterologyFissure in anolaw.inventionLeukocyte CountFeeding behaviorRandomized controlled trialDouble-Blind MethodlawRecurrenceInternal medicinemedicineHumansLymphocyte CountLymphocytesIntestinal MucosaTriticumHepatologybusiness.industryGastroenterologyFeeding BehaviorMiddle AgedMilk ProteinsSettore MED/45 - Scienze Infermieristiche Generali Cliniche E PediatricheFood hypersensitivitySurgeryEosinophilsSettore MED/18 - Chirurgia GeneraleChronic diseaseTreatment OutcomeChronic DiseasefissureFemaleFissure in Anohypersensitivitymedicine.symptomMilk HypersensitivitybusinessConstipationFood HypersensitivityThe American journal of gastroenterology
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Comparison of topical tacrolimus 0.1 % in pectin ointment with clobetasol 0.5% ointment in adults with moderate to severe desquamative gingivitis: A …

2006

BACKGROUND: Desquamative gingivitis (DG) is a clinical condition characterized by red, painful, glazed, and friable gingiva, which might be a manifestation of some autoimmune mucocutaneous diseases. The time from the development of initial signs of DG to diagnosis can vary from months to years. Based on a literature search, no data concerning patients with DG without signs of autoimmune disease were available. OBJECTIVE: The aim of this trial was to compare the efficacy and tolerability of monotherapy with topical tacrolimus 0.1% in pectin ointment versus clobetasol propionate 0.5% ointment in adults affected by DG. METHODS: This randomized, double-blind clinical trial was conducted at the …

AdultMalemedicine.medical_specialtySettore MED/09 - Medicina InternaErythemaAdministration TopicalPopulationSeverity of Illness IndexTacrolimuslaw.inventionOintmentsRandomized controlled trialDouble-Blind Methodlawdesquamative gingivitimedicineClinical endpointHumansPharmacology (medical)educationAgedPharmacologyeducation.field_of_studyDose-Response Relationship Drugbusiness.industryMiddle Agedmedicine.diseaseDermatologyGingivitisdesquamative gingivitisTacrolimusSurgeryDesquamative gingivitisTreatment OutcomeTolerabilityclobetasoltopical tacrolimus; clobetasol; desquamative gingivitis; randomized double-blind clinical trialtacrolimus clobetasol desquamative gingivitisPectinsDrug Therapy CombinationFemaleClobetasol propionatemedicine.symptombusinessImmunosuppressive Agentsmedicine.drugFollow-Up Studiestopical tacrolimurandomized double-blind clinical trial
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Fecal assays detect hypersensitivity to cow's milk protein and gluten in adults with irritable bowel syndrome.

2011

Background & Aims Some patients with irritable bowel syndrome (IBS)-like symptoms suffer from food hypersensitivity (FH); their symptoms improve when they are placed on elimination diets. No assays identify patients with FH with satisfactory levels of sensitivity. We determined the frequency of FH among patients with symptoms of IBS and the ability of fecal assays for tryptase, eosinophil cationic protein (ECP), or calprotectin to diagnose FH. Methods The study included 160 patients with IBS, 40 patients with other gastrointestinal diseases, and 50 healthy individuals (controls). At the start of the study, patients completed a symptom severity questionnaire, fecal samples were assayed, and …

AdultMalemedicine.medical_specialtySettore MED/09 - Medicina InternaFecal Calprotectin.AdolescentGlutensTryptaseWheat HypersensitivityGastroenterologyIrritable Bowel SyndromePlacebosFecesYoung Adultfluids and secretionsDouble-Blind MethodInternal medicineElimination dietmedicineHumansIrritable bowel syndromeFeceschemistry.chemical_classificationEosinophil cationic proteinHepatologybiologybusiness.industryOral food challengeEosinophil Cationic ProteinGastroenterologyMiddle Agedmedicine.diseaseGlutenDietchemistryGluten SensitivityImmunologybiology.proteinFemaleTryptasesCalprotectinCow’s Milk Protein HypersensitivityMilk HypersensitivitybusinessLeukocyte L1 Antigen ComplexCow’s Milk Protein Hypersensitivity; Gluten Sensitivity; Fecal Tryptase; Fecal Eosinophil Cationic Protein; Fecal Calprotectin.Fecal TryptaseFecal Eosinophil Cationic ProteinClinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association
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Antihypertensive efficacy and effects of nitrendipine on cardiac and renal hemodynamics in mild to moderate hypertensive patients: Randomized control…

1992

In this study antihypertensive efficacy, safety, and the effects of short-term nitrendipine administration on central and renal hemodynamics were evaluated in mild to moderate hypertensives. Our final goal was to ascertain whether the reduction in blood pressure induced by nitrendipine treatment was associated with maintained renal function. After a run-in period with placebo, 26 hypertensives without cardiac or renal disease were randomly assigned to a double-blind 8-week controlled trial with nitrendipine (N) 20 mg once a day (13 pts) or hydrochlorothiazide (HCT) 25 mg once a day (13 pts). Renal hemodynamic measurements included effective renal plasma flow (ERPF) and glomerular filtration…

AdultMalemedicine.medical_specialtySettore MED/09 - Medicina InternaHemodynamicsRenal functionKidneyHydrochlorothiazideDouble-Blind MethodNitrendipineInternal medicinemedicineHumansPharmacology (medical)PharmacologyEjection fractionbusiness.industryNitrendipineHemodynamicsGeneral MedicineEffective renal plasma flowMiddle AgedSettore MED/11 - Malattie Dell'Apparato CardiovascolareFiltration fractionHydrochlorothiazideEndocrinologyRenal blood flowHypertensionSettore BIO/14 - FarmacologiaCardiologyFemaleCalcium channel blockers nitrendipine essential arterial hypertension cardiac function renal function.Cardiology and Cardiovascular Medicinebusinessmedicine.drugCardiovascular Drugs and Therapy
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Beclomethasone dipropionate in Crohn's ileitis: A randomised, double-blind trial.

2011

Abstract Background Steroids, the mainstay of Crohn's disease treatment, have been associated with systemic side effects. Aim To evaluate the efficacy and tolerability of beclomethasone dipropionate for maintaining remission induced by a short course of systemic steroids in patients with Crohn's ileitis with or without right colonic involvement. Methods Patients (n = 84) with active Crohn's disease who achieved remission during a 2-week prednisone run-in period were randomised to receive beclomethasone dipropionate for 24 weeks or continue prednisone for a further 2 weeks followed by placebo for 22 weeks. The primary outcome was relapse rate (Crohn's Disease Activity Index score > 150 and a…

AdultMalemedicine.medical_specialtySettore MED/09 - Medicina InternaRemissionKaplan-Meier EstimateBeclomethasone dipropionate Crohn's disease RemissionPlaceboGastroenterologyDrug Administration ScheduleMedication Adherencelaw.inventionDouble blindCrohn DiseaseDouble-Blind MethodRandomized controlled triallawPrednisoneInternal medicineSecondary PreventionmedicineHumansIleitisAdverse effectGlucocorticoidsAgedCrohn's diseaseHepatologybusiness.industryRemission InductionBeclomethasoneGastroenterologyBeclomethasone dipropionate Crohn's ileitis:.Middle Agedmedicine.diseaseSurgeryBeclomethasone dipropionateCrohn's diseaseTreatment OutcomeTolerabilityPrednisoneFemaleTablets Enteric-Coatedbusinessmedicine.drug
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Effects of cilazapril on renal haemodynamics and function in hypertensive patients: a randomised controlled study versus hydrochlorothiazide.

1995

In this study the efficacy and safety of short-term cilazapril administration on renal haemodynamics were evaluated in mild to moderate hypertensive subjects. Our final goal was to evaluate whether the reduction in blood pressure achieved by treatment was associated with maintained renal function. After a run-in period with placebo, 40 hypertensive subjects without renal or cardiac diseases were randomly allocated to a double-blind 4 week controlled trial with cilazapril 5 mg once a day (20 patients) or hydrochlorothiazide 25 mg once a day (20 patients). Renal haemodynamics measurements included effective renal plasma flow (ERPF) and glomerular filtration rate (GFR) by radionuclide study us…

AdultMalemedicine.medical_specialtySettore MED/09 - Medicina InternaUrologyRenal functionAngiotensin-Converting Enzyme InhibitorsCilazaprilEssential hypertensionRenal CirculationHydrochlorothiazideDouble-Blind MethodInternal medicineInternal MedicinemedicineAngiotensin-Converting Enzyme InhibitorsAntihypertensive Agents Hydrochlorothiazide CilazaprilHumansAntihypertensive AgentsAgedRenal circulationbusiness.industryHemodynamicsGeneral MedicineEffective renal plasma flowCilazaprilMiddle Agedmedicine.diseaseSettore MED/45 - Scienze Infermieristiche Generali Cliniche E PediatricheSettore MED/11 - Malattie Dell'Apparato CardiovascolareFiltration fractionmedicine.anatomical_structureEndocrinologyHydrochlorothiazideTreatment OutcomeRenal blood flowHypertensionSettore BIO/14 - FarmacologiaFemaleCardiology and Cardiovascular Medicinebusinessmedicine.drugGlomerular Filtration RateBlood pressure
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Comparative effect of tacrolimus 0.1% ointment and clobetasol 0.05% ointment in patients with oral lichen planus.

2008

Background Oral lichen planus (OLP) is considered to be an autoimmune disease of unknown aetiology that affects the mucosae, especially the oral cavity. Objective We compared tacrolimus 0.1% ointment and clobetasol 0.05% ointment for the treatment of OLP. Patients and methods A total of 32 patients (20 females and 12 males; all white, Italian origin, mean age of 43.6+/-18.4 years; 16 patients per treatment group) were treated with tacrolimus or clobetasol ointment for 4 weeks in a randomized, double-blind, clinical trial. Pain severity, burning sensation, and mucosal lesion extension were assessed using a four-point scale. Results At the end of the treatment period, symptom scores were sign…

AdultMalemedicine.medical_specialtySettore MED/09 - Medicina Internamedicine.drug_classAnti-Inflammatory Agentstacrolimus ointment; clobetasol ointment; oral lichen planuslaw.inventionOintment Basesoral lichen planusRandomized controlled triallawOral administrationFacial PainmedicineHumanstacrolimus ointmentSalivatacrolimusbusiness.industryclobetasol ointmentclobetasol; tacrolimus; oral lichen planusmedicine.diseaseDermatologyTacrolimusCalcineurinstomatognathic diseasesclobetasolOintment BasesPeriodonticsCorticosteroidOral lichen planusFemaleClobetasol propionatebusinessEpidemiologic MethodsImmunosuppressive Agentsmedicine.drugLichen Planus Oral
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Bone Marrow Concentrate and Bovine Bone Mineral for Sinus Floor Augmentation

2011

Purpose: The purpose of this work was to evaluate the potential of substituting autogenous bone (AB) by bone marrow aspirate concentrate (BMAC). Both AB and BMAC were tested in combination with a bovine bone mineral (BBM) for their ability of new bone formation (NBF) in a multicentric, randomized, controlled, clinical and histological noninferiority trial.Materials and Methods: Forty-five severely atrophied maxillary sinus from 26 patients were evaluated in a partial cross-over design. As test arm, 34 sinus of 25 patients were augmented with BBM and BMAC containing mesenchymal stem cells. Eleven control sinus from 11 patients were augmented with a mixture of 70% BBM and 30% AB. Biopsies wer…

AdultMalemedicine.medical_specialtySinus Floor AugmentationOSTEOGENIC DIFFERENTIATIONBone RegenerationMaxillary sinusBiomedical EngineeringSinus Floor AugmentationBioengineeringBiochemistryMAXILLARY SINUSlaw.inventionMESENCHYMAL STEM-CELLSBIO-OSSBiomaterialsRandomized controlled trialTISSUE-ENGINEERED BONEBone MarrowOsteogenesislawmedicineAnimalsHumansSingle-Blind MethodSinus (anatomy)AgedIMPLANT PLACEMENTHYDROXYAPATITE CERAMICSBone TransplantationIntention-to-treat analysisPOROUS HYDROXYAPATITEbusiness.industryIN-VITROMiddle AgedSurgeryAUTOGENOUS BONEImplant placementBovine bonemedicine.anatomical_structureBone SubstitutesCattleFemaleBone marrowbusinessTissue Engineering. Part A
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