Search results for " microbiologia"
showing 10 items of 907 documents
Use of pulsed field gel electrophoresis (PFGE)and single-enzyme amplified fragment length polymorphism (SE-AFLP) to subtype isolates of Salmonella en…
2005
Recent trends in salmonellosis epidemiology
1999
The taxonomy of the genus Salmonella and the recent trends in the epidemiology of salmonellosis have been reviewed. Global and Italian trends in salmonellae infections have been considered focusing on the application of molecular biology methods to the study of epidemic isolates, as in the case of S. Wien and the more recent S. Enteritidis pandemics. Interventions for salmonellosis prevention, and control measures including new rapid cultural methods for the detection of salmonellae in foods, have been summarized.
Molecular analyses on HPV infections in semen
Introduction: Human Papillomaviruses (HPVs) are non-enveloped double stranded DNA viruses classified in different genera and several different genotypes. Depending on the genotypes, HPV infections can be asymptomatic or can cause from warts to malignant tumours. Some genotypes, such as HPV18 and HPV16, are considered high risk (HR) HPVs and they are the major cause of cervical cancer. HPV is highly tissue-tropic and infects epithelial cells, but it can also binds other cell types. It is known that HPV can be found in semen but its effects on spermatozoa and male reproductive system are not completely clarified. Materials and Methods: In order to study the HPV infection in semen, we develope…
Evaluation of irreversible dynamic thermoporation as a tool for the deduction of bacterial load in food matrices (Note) [Valutazione della termoporaz…
2015
La termoporazione dinamica irreversibile (DIT) consiste in un nuovo tipo di tecnologia di pastorizzazione ideato da Koulik e colleghi basato su un processo termico dinamico, caratterizzato da riscaldamenti molto rapidi (shock termici) a temperature finali non superiori a 70°C. L’obiettivo del nostro studio è stato la valutazione dell’influenza di parametri di processo quali la temperatura di partenza (T1 ) del campione da trattare, la temperatura finale in corso di trattamento (T 2), la differenza tra temperatura iniziale e temperatura finale (ΔT), la velocità di riscaldamento (θ) e la durata del trattamento sull’abbattimento della carica batterica nella matrice alimentare
Detection of the norovirus variants GGII.4 hunter and GGIIb/hilversum in Italian children with gastroenteritis.
2006
Noroviruses (NoVs) are important enteric pathogens of humans. Although they exhibit an impressive genetic diversity, few NoV strains appear to predominate worldwide. Limited epidemiological data are available on NoV gastroenteritis in Italy. In this study, we assessed the prevalence of human NoV in Italian children with gastroenteritis by using a reverse-transcription nested polymerase chain reaction (RT-PCR) assay specific for the RNA-dependent RNA polymerase (RdRp) on faecal samples collected throughout the 2004 surveillance activity in Palermo, Italy. NoVs were detected in 47% of the stool samples obtained from children <5 years age, admitted to hospital with acute non-bacterial gastroen…
Rapidly growing mycobacteria in TB/HIV co-infection: a report of two cases focusing on difficulties in diagnosis and management.
2012
Recent reports indicate an increase in rates of infection and disease due to rapidly growing mycobacteria (RGM) in patients with pre-existing chronic lung disease. Studies have described difficulties in correctly identifying closely related species, even when proper methodologies are adopted, and several different gene targets have been proposed. We describe two cases of RGM infection in a 29-year-old HIV-1 positive Congolese man and a 19-year-old HIV-1 positive Liberian woman, respectively, both with bronchiectasis due to previous Mycobacterium tuberculosis (MTB) infection. Mycobacterium porcinum and Mycobacterium bolletii were identified in bronchoalveolar lavage fluid and sputum, respect…
Sorveglianza molecolare delle gastroenteriti da rotavirus in Sicilia
2013
A case of spotted fever rickettsiosis in a HIV-positive patient
2013
Polyclonal non multiresistant Staphylococcus aureus isolates from clinical cases of infection occurring in Palermo, Italy, during a one-year surveill…
2012
BACKGROUND: The evolving epidemiology of methicillin resistant Staphylococcus aureus (MRSA) is characterized by the emergence of infections caused by non multiresistant MRSA carrying staphylococcal chromosomal cassette (SCC)mec IV or V in the healthcare settings. A molecular epidemiological analysis of non multiresistant MRSA isolates from four acute general hospitals was performed in Palermo, Italy, during a one year period. METHODS: For the purpose of the study, MRSA isolates were defined as non multiresistant when they were susceptible to at least three classes of non beta-lactam antibiotics. Seventy-five isolates were submitted to antimicrobial susceptibility testing, multilocus sequenc…
Surveillance of enteric virus infections in a neonatal intensive care unit.
2005
Objective. To investigate the epidemiology of neonatal viral gastroenteritis compared to the circulation of enteric viruses in children, 109 newborns in the NICU of Mother and Child Department and 214 children with enteritis admitted to the “G. Di Cristina” Children’s Hospital in Palermo were monitored for Rotavirus, Adenovirus, Astrovirus and Norovirus infections. Methods. Stool samples were examined by EIA to detect viral antigens. Rotavirus strains were subjected to P- and G-typing. Results. A Norovirus strain was detected in one neonatal stool specimen whereas an astrovirus strain was dectected in two neonatal specimens. No Rotavirus or Adenovirus infection was identified among the newb…