Search results for " microorganism"
showing 10 items of 81 documents
Levels of predominant intestinal microorganisms in 1 month-old full-term babies and weight gain during the first year of life
2021
The early life gut microbiota has been reported to be involved in neonatal weight gain and later infant growth. Therefore, this early microbiota may constitute a target for the promotion of healthy neonatal growth and development with potential consequences for later life. Unfortunately, we are still far from understanding the association between neonatal microbiota and weight gain and growth. In this context, we evaluated the relationship between early microbiota and weight in a cohort of full-term infants. The absolute levels of specific fecal microorganisms were determined in 88 vaginally delivered and 36 C-section-delivered full-term newborns at 1 month of age and their growth up to 12 …
Trend of MDR-microorganisms isolated from the biological samples of patients with HAI and from the surfaces around that patient.
2018
Healthcare-associated infections (HAI) continue to be a major public health concern. A number of epidemiologically relevant HAI microorganisms are multidrug-resistant (MDR) germs that can spread rapidly and/or carry multiple resistance to antibiotics. They are the cause of high mortality and possible nosocomial epidemics. For this reason, we implemented microbiological surveillance acquiring samples from patients with HAI and environmental samples from the surfaces surrounding those patients. A retrospective study was carried out from January 2014 to December 2016 in two departments of the University Hospital in Messina, Italy: the Microbiology and the Hygiene Laboratories. A comparison was…
Restoration practices in Mediterranean habitats using native woody species
2017
Since the beginning of the XXI century, Legambiente (a national environmental association), supported by the University of Palermo, has launched several naturalization projects within three Sicilian nature reserves: 1)Isola di Lampedusa (Agrigento Province); 2)Macalube di Aragona (Agrigento Province); 3)Lago Sfondato (Caltanissetta Province). Interventions were carried out on bare lands and degraded sites where natural vegetation cover was almost completely disappeared. The main aim was to restore native habitats following the principles of ecological restoration. Accordingly, differently from the classical approach, consisting in the use of preparatory species, usually Pines, native shrubs…
Experimental Evaluation of a New Thermal Process for Microorganisms Inactivation
2015
A new thermal process for the inactivation of microorganisms in beverages has been studied and is presented in this paper. The treatment, not yet studied in the scientific literature, mainly consists of a thermal shock characterized by temperature increases up to 30°C/s, with final temperatures up to 65°C. This study presents the first experimental results obtained by the application of the new thermal treatment, with different combinations of the process parameters (rate of temperature rise, final temperature and holding time), on separate suspensions of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Listeria innocua and Candida albicans. The required rapid temperature in…
Advanced Strategies for Food-Grade Protein Production: A New E. coli/Lactic Acid Bacteria Shuttle Vector for Improved Cloning and Food-Grade Expressi…
2019
Food-grade production of recombinant proteins in Gram-positive bacteria, especially in LAB (i.e., Lactococcus, Lactobacillus, and Streptococcus), is of great interest in the areas of recombinant enzyme production, industrial food fermentation, gene and metabolic engineering, as well as antigen delivery for oral vaccination. Food-grade expression relies on hosts generally considered as safe organisms and on clone selection not dependent on antibiotic markers, which limit the overall DNA manipulation workflow, as it can be carried out only in the expression host and not in E. coli. Moreover, many commercial expression vectors lack useful elements for protein purification. We constructed a &ld…
Human Monkeypox: A Comprehensive Narrative Review and Analysis of the Public Health Implications.
2022
Recently, numerous cases of monkeypox were reported from several non-endemic countries in Europe, North America, and Oceania, suggesting an unusual and alarming public health issue, particularly considering that the disease is not directly related to human or animal travels. Attention is currently being drawn to this phenomenon since more than 70% of the global population is no longer vaccinated against smallpox. Indeed, the smallpox vaccination also confers some indirect degree of protection against other poxviruses, including monkeypox. We performed a narrative review to describe the existing literature with regard to monkeypox using the MEDLINE, EMBASE, and Scopus databases. This review …
Documento de consenso sobre el tratamiento antimicrobiano de las infecciones bacterianas odontogénicas
2005
Las infecciones de la cavidad bucal son un problema de salud pública frecuente y motivo constante de prescripción antibiótica; el 10% de los antibióticos se emplean para tratar este problema. Sin embargo, hasta la fecha son pocos los estudios realizados para determinar su incidencia. Asímismo, su relación con ciertas enfermedades sistémicas (cardiacas, endocrinas, etc...) confiere a estas patologías una importancia vital. A pesar de la reconocida frecuencia e importancia de las infecciones odontogénicas, llama la atención la actual dispersión de criterio en varios aspectos referentes a su clasificación, terminología y recomendaciones terapéuticas. El objetivo principal de este documento, re…
Omics approaches to study the oral microbiome
2015
Introducción La visión clásica de los microorganismos como agentes infecciosos ha propiciado que se consideren como meros agentes causales de enfermedades. Sin embargo, la larga historia de coexistencia entre microorganismos y los seres humanos, ha hecho que ambos se hayan adaptado mutuamente y coevolucionen (Dubos et al. 1965, McFall-Ngai 2002). De hecho, en el cuerpo humano habitan 1014 células bacterianas, un orden de magnitud más que células humanas (Savage 1977). Entre los beneficios que la microbiota aporta, están su contribución a digerir alimentos y a aportar nutrientes, regulación del metabolismo, maduración del sistema inmune, etc. Por ello, hoy en día las enfermedades de origen…
Hacia el entendimiento del control de la expresión génica del regulón clanobacteriano del nitrógeno mediado por el factor de transcripción NtcA y la …
2018
NtcA, es un factor de transcripción cianobacteriano perteneciente a la familia CRP. Es considerado el regulador global del nitrógeno en cianobacterias ya que presenta un regulón muy amplio que incluye muchos genes implicados en el metabolismo de nitrógeno. Se activa por 2-oxoglutarato(2-OG), un indicador del nivel de nitrógeno celular y es coactivado por una proteína llamada PipX, objeto importante de este estudio. A bajo nivel de 2-OG, PipX es secuestrada por la proteína señalizadora PII quedando inaccesible para activar NtcA. Sin embargo, la unión de 2-OG a PII en condiciones de escasez de nitrógeno, libera PipX, la cual queda accesible para coactivar NtcA. Las estructuras de NtcA unido a…
ENHANCEMENT OF WHEAT VIRUS-RESISTANCE AT APPLICATION OF THE SE NANOPARTICLES CITRATES AND CONSORTIUM OF SOIL MICROORGANISMS
2021
The influence of pre-sowing seed treatments of selenium nanocitrates (SeNPs) and application of soil formed microorganism consortium (biological preparation (BP) Extrakon) on wheat plants infected with wheat streak mosaic virus (WSMV) were investigated in greenhouse and fields conditions in 2018-2019 (on territories of Zabolotny Institute of Microbiology and Virology of NASU). The pre-sowing seed treatments of 1% selenium nanocitrates (SeNPs) with application of BP Extrakon initiating the growth of juvenile wheat in laboratory experiments were found. In the field experiment on variants of WSMV-infected plants, two weeks after inoculation (tillering stage), we observed the appearance of char…