Search results for " miglioramento"
showing 10 items of 219 documents
Proteomics for milk proteins characterization in Girgentana goat breed
2013
Proteomics has been widely used to characterize milk protein content in livestock species and unravel protein biological functions. The aim of this work was to characterize the Girgentana goat breed whole milk and milk fraction proteins by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry. Bulk milk samples from two farms locate in Palermo and Agrigento (Sicily) areas were collected during the early stage of lactation. Initially, 2D-PAGE and protein detection protocols were set-up. In particular, 2D-PAGE analyses were carried out on whole milk using 18-cm IPG strips ranging from pH 3-10 and 4-7 and 12.5% polyacrylamide gel for first and the second dimension …
Detection of genomic signatures of selection in the North African Brune de l’Atlas cattle
2018
The identification of signatures of selection in indigenous livestock genome is able to elucidate the adaptive mechanisms allowing them to cope with environmental pressures. In the present study, we used genotyping data from 106 Brune de l’Atlas cattle individuals and tests based on the extent of haplotype homozygosity (EHH) to look for footprints of natural selection. We identified 17 genomic regions displaying strong signal of selection (p-value <10-6) overlapping with previously described QTL regions affecting many traits such as meat and milk composition, tuberculosis susceptibility, tick and mastitis resistance. Some of these highly significant candidate regions overlap with four previ…
Selection signatures of fat tail in sheep
2017
The investigation of the genes with a role in lipid metabolism enjoy considerable scientific and commercial interest because of the strong correlations between fat deposition and the risk of cardiovascular disease. The fat tail characteristic of sheep is the adaptive response to harsh environment, and beyond representing a valuable energy reserve for facing future climate changes provides clues for elucidating the physiology of fat deposition. Studies on various sheep populations detected fat-tail signatures on chromosomes 2, 3, 5, 6, 7 and 13. Fat-tailed sheep represent about 25% of the world’s sheep population, and the genes with a role in this phenotype are likely not the same for every …
Application of molecular markers to investigate genetic diversity in Sicilian livestock
2014
La diversità genetica delle specie e razze di interesse zootecnico, rappresenta un’importante risorsa in tutti i sistemi di allevamento . Per lo studio della diversità genetica, nel corso dei decenni sono stati sviluppati diversi metodi che si basano su informazioni del pedigree o su dati molecolari (microsatelliti e SNPs, Single Nucleotide Polymorphisms). Con l’aumento della disponibilità di marcatori molecolari per la maggior parte delle specie di interesse zootecnico, e con lo sviluppo di sofisticate tecniche analitiche, sta crescendo la capacità di caratterizzare la variabilità genetica delle razze. Inoltre, ad oggi, poche sono le informazioni sulla diversità genetica delle razze e dell…
A genome-wide perspective on the population structure of Italian cattle breeds
2018
Despite the growing diffusion of cosmopolite specialized breeds, several autochthonous cattle breeds and populations are still bred in Italy. The aim of this study was to provide a high-resolution picture of the genome-wide diversity and population structure of Italian local cattle breeds using medium-density genome-wide SNP markers. After data editing, the dataset included 800 samples from 32 breeds that were genotyped for 31 013 SNPs. For several breeds we observed a low level of polymorphism and genetic diversity, that together with the small effective population sizes confirmed threat of extinction. According to the runs of homozygosity analysis, evidence of recent inbreeding was strong…
Studio del gene della β-lattoglobulina in razze ovine e caprine autoctone siciliane
2009
Identificazione di una nuova variante alla κ-caseina nella razza caprina Girgentana
2013
La κ-caseina è la lattoproteina che determina la grandezza e la funzione specifica delle micelle nel latte e la sua idrolisi, chimosina dipendente, è responsabile della coagulazione del latte stesso. Il gene della κ-caseina comprende 5 esoni. Ad oggi sono state identificate 16 varianti alleliche, di cui 13 sono varianti proteiche e 3 mutazioni silenti, per un totale di 15 siti polimorfici.Lo scopo di questo lavoro è stato la caratterizzazione dell’esone 4 del gene della κ-caseina nella razza caprina Girgentana. Un nuova variante alleliche, denominata X, è stata riscontrata con una frequenza relativamente bassa (0,04).
Effect of muscle type and animal category on fatty acid composition of bresaola made from meat of Cinisara cattle: preliminary investigation
2020
The bresaola could represent an alternative commercial opportunity for autochthonous dairy cattle farms. Therefore, a study was carried out to investigate the fatty acid (FA) composition of bresaola made using Semimembranosus (SMm), Semitendinosus (STm), and Biceps brachii (BBm) muscles from grazing young bulls (GB), housed young bulls (HB) or grazing adult cows (AC) of Cinisara breed. Animal category, fat content, feeding system, and type of muscle influenced the FA composition of bresaola. Fatter products, made from AC and HB meat, or with STm and BBm muscles showed lower polyunsaturated/saturated FA ratio. The bresaola from young bulls fed pasture-based diet showed an increased polyunsat…
Sostenibilità di interventi di miglioramento sismico di strutture in c.a. non danneggiate
2012
Interventi di miglioramento e adeguamento sismico di edifici in c.a. dell’Azienda Ospedaliera Universitaria Policlinico “Paolo Giaccone” di Palermo
2008
The paper shows the results of an experimental investigation on the behaviour of compressed concrete columns subjected to the overcoring technique. Extraction of cylindrical concrete cores in perpendicular direction to the column surface, was achieved by rotary drilling using a diamond tipped hollow barrel. Core is gradually isolated from the stress field in the surrounding concrete and at every advancing step the re-equilibrium deformation response is measured by means of four strain-gauges, placed on its external surface and near the hole. Finally the original stress state is computed in the elastic phase on the basis of recorded strains, knowing the elastic properties of concrete and by …