Search results for " multiplex"
showing 10 items of 158 documents
Results of collaborative study regarding the standardization of the Y-linked STR system DYS385 by the European DNA Profiling (EDNAP) group.
1999
Y-chromosome linked short tandem repeat (STR) loci are inherited as a closely linked haplotype, which appears to remain stable in a given paternal lineage over many generations. In forensic cases, Y-linked STRs are particularly useful for the identification of human remains as well as in rape cases with mixed male/female stain samples. DYS385 is derived from tandemly duplicated segments of the Y chromosome thus giving rise to two fragments of variable length which do not behave like alleles but genotypes. The European DNA Profiling (EDNAP) group has carried out a collaborative exercise among 14 participating laboratories using DYS385 for typing of five unknown bloodstains and a control samp…
Characterization of mutations and sequence variations in complex STR loci by second generation sequencing
2013
Abstract We used second generation sequencing (SGS) to examine sequence variation and mutational patterns in complex STR loci in Danish paternity cases. Four STR loci, D2S1338, D3S1358, D12S391 and D21S11, were sequenced in 45 samples from 15 confirmed trios with genetic inconsistencies between one of the parents and the child in either the D2S1338 or the D3S1358 loci. In 12 of the 15 cases, SGS revealed the allele that had mutated from the parent. In total, 61 different alleles were found in the 30 unrelated individuals. The highest variation was observed in the D12S391 locus and the lowest one in the D3S1358 locus. The four STR loci are good candidate loci for future SGS kits for forensic…
Whole genome amplification—the solution for a common problem in forensic casework?
2004
Abstract To assess the quality of amplified DNA obtained by whole genome amplification, 17 independent STR loci have been typed using two multiplex kits. Results have been compared for correct genotypes, heterozygous peak balance and allelic dropout.
Development of a quadruplex PCR system for the genetic analysis of X-chromosomal STR loci
2003
Short tandem repeat systems on the X chromosome are the natural counterpart to the well-established Y-chromosomal STR loci. The X-linked systems are inherited as a single haplotype only in males, whereas in females, the X chromosomes recombine and exhibit the same characteristics as the autosomes. Nevertheless, X-linked systems may provide a useful tool in paternity cases with female offspring, in particular when the alleged father is not available for testing, or in forensic identification cases based on the comparison with firstor second-degree relatives. Only a small number of STR loci have been described on the X chromosome, and a number of these are not highly informative. Therefore, w…
Multiplex ligation-dependent probe amplification detection of an unknown large deletion of the CREB-binding protein gene in a patient with Rubinstein…
2013
Rubinstein-Taybi syndrome is a rare autosomal dominant congenital disorder characterized by postnatal growth retardation, psychomotor developmental delay, skeletal anomalies, peculiar facial morphology, and tumorigenesis. Mutations in the gene encoding the cAMP response element-binding protein (CREB, also known as CREBBP or CBP) on chromosome 16p13.3 have been identified. In addition, some patients with low intelligence quotients and autistic features bear large deletions. Based on these observations, we used multiplex ligation-dependent probe amplification to search for large deletions affecting the CREBBP gene in a Rubinstein-Taybi syndrome patient. We identified a novel heterozygote dele…
Analysis of duplexing modes in the IEEE 802.16 wireless system
2010
The IEEE 802.16 standard defines two main duplexing modes: Time Division Duplexing (TDD) and Frequency Division Duplexing (FDD). The FDD can be divided further into Full-FDD (F-FDD) and Half-FDD (H-FDD). F-FDD requires full duplexing support from subscriber stations and works with two frequency bands. TDD operates a single frequency band, but it does not need full-duplexing support. H-FDD is a combination of these: it works with two frequency bands and does not require full-duplexing support. The cost of this is a more complicated scheduling and added overhead. Still, H-FDD might be the only possible duplexing mode in some occasions. In this paper, these duplexing modes are compared and the…
Super resolving optical system based on spectral dilation
2004
Time multiplexing is a common approach for achieving super resolution. The basic method involves moving two grating one is in front of the object and the other one in front of the camera. In this paper, we present a novel approach for obtaining super resolution not by shifting a grating, but by using its various dilations for obtaining the required encoding and decoding of information.
Joint Distribution of Polarization-Multiplexed UWB and WiMAX Radio in PON
2009
[EN] In this paper, the feasibility of the joint distribution of ultra-wideband (UWB) and WIMAX wireless using polarization multiplexing as a coexistence technique is proposed and experimentally demonstrated within the framework of passive optical networks (PON). Four single- and orthogonal-polarization multiplexing schemes are studied targeting to reduce the mutual interference when UWB and WiMAX are distributed jointly through standard single-mode fiber (SSMF) without transmission impairments compensation techniques and amplification. Experimental results indicate successful transmission up to 25 km, in SSMF exceeding the range in typical PON deployments. The radio link penalty introduced…
Secondary users selection and sparse narrow-band interference mitigation in cognitive radio networks
2018
Spectrum scarcity is a critical problem that may reduce the effectiveness of wireless technologies and services. To address this problem, different spectrum management techniques have been proposed in the literature such as overlay cognitive radio (CR) where the unlicensed users can share the same spectrum with the licensed users. The main challenges in overlay CR networks are the identification and detection of the Primary User (PU) signals in a multi-source narrow-band interference (NBI) scenario. Therefore, in this paper, we investigate the performance of an orthogonal frequency division multiplexing (OFDM) overlay CR network with Secondary Users (SUs) and subcarriers selection schemes. …
Giarraffa and Grossa di Spagna naturally fermented table olives: Effect of starter and probiotic cultures on chemical, microbiological and sensory tr…
2014
Abstract This work investigated on the effects of selected starters and of probiotic strains on the evolution of microbiota, on trend of volatile compounds and on the final sensorial traits of green table olives. The olives, belonging to Giarraffa and Grossa di Spagna cultivars, were processed according to Sicilian traditional method and the following samples were obtained: (1) un-inoculated spontaneous olives, control; (2) olives inoculated with probiotic Lactobacillus rhamnosus H25; (3) olives inoculated with commercial probiotic L. rhamnosus GG; (4) olives inoculated with Lactobacillus plantarum GC3 plus Lactobacillus paracasei BS21; and (5) olives inoculated with L. plantarum GC3 plus L…