6533b86ffe1ef96bd12cd191

RESEARCH PRODUCT

Development of a quadruplex PCR system for the genetic analysis of X-chromosomal STR loci

Peter M. SchneiderD. AthanasiadouK.w. AltCh. RittnerBeate Stradmann-bellinghausen

subject

Geneticseducation.field_of_studyAutosomeSTR multiplex systemPopulationHaplotypeMicrosatelliteLocus (genetics)General MedicineBiologyeducationGenetic analysisX chromosome

description

Short tandem repeat systems on the X chromosome are the natural counterpart to the well-established Y-chromosomal STR loci. The X-linked systems are inherited as a single haplotype only in males, whereas in females, the X chromosomes recombine and exhibit the same characteristics as the autosomes. Nevertheless, X-linked systems may provide a useful tool in paternity cases with female offspring, in particular when the alleged father is not available for testing, or in forensic identification cases based on the comparison with firstor second-degree relatives. Only a small number of STR loci have been described on the X chromosome, and a number of these are not highly informative. Therefore, we have investigated the four X-chromosomal STR loci: DXS101, DXS8377, HPRTB and STRX-1 [1–5], to develop a rapid multiplex PCR typing system suitable for fluorescent detection and to perform population genetic studies among Germans. Whereas HPRTB and STRX-1 contain tetrameric repeats, DXS101 and DXS8377 have trimeric repeats. All four loci are highly polymorphic exhibiting up to 18 common alleles at each locus (Table 1).

https://doi.org/10.1016/s0531-5131(02)00479-x