Search results for " orange"
showing 10 items of 99 documents
Genetic analysis of Citrus aurantium L. (Rutaceae) cultivars by ISSR molecular markers
2011
Eight cultivars of Citrus aurantium L., showing peculiar morphological traits of the fruits and leaves, were analyzed genetically. Inter simple sequence repeat (ISSR) was chosen as molecular markers because they represent a highly efficient system for investigating variability at intraspecific level. The particular morphological traits were discussed, the genetic identity and distance matrix based on Nei index was calculated, and the unweighted pair group method with arithmetic mean (UPGMA) dendrogram was generated. A total of 53 clearly distinguished DNA bands were considered for the ISSR analysis of which 24 were polymorphic. A basic C. aurantium fingerprinting pattern was obtained. The I…
Activation of a caspase-3-independent mode of cell death associated with lysosomal destabilization in cultured human retinal pigment epithelial cells…
2008
International audience; Purpose: To characterize the possible cytotoxic effects of oxysterols (7-hydroxycholesterol (7-OH), 25-hydroxycholesterol (25-OH)) in human retinal pigment epithelial cells (ARPE-19) and to detail the relationships between some of these effects. Methods: ARPE-19 cells were treated with 7-OH and 25-OH. Cell viability was measured with the MTT assay. Membrane permeability, mitochondrial potential, and lysosomal integrity were measured by flow cytometry with propidium iodide, DiOC6(3), and acridine orange, respectively. Cell death was characterized by staining with Hoechst 33342, transmission electron microscopy, and analysis of the DNA fragmentation pattern. Caspase ac…
High resolution analysis and differential condensation in RBA-banded human chromosomes.
1981
Human prophase, premetaphase, and mid-metaphase chromosomes are prepared and analyzed using the thymidine cell synchronization technique and R-banding patterns (RBA). Haploid sets with 700–1000 bands can be demonstrated. Sequences of chromosomes of different degrees of condensation are helpful for a better understanding and classification of regions of extended chromosomes. A considerable variation in the condensation of parts of homologous chromosomes is reflected in the variability of the arm ratio. This differential condensation of chromosomes is entirely effected by variation of the degree of condensation in AT rich interbands and can be attributed to the degree of labeling by BrdU.
A novel magnetic Preyssler acid grafted chitosan nano adsorbent: synthesis, characterization and adsorption activity
2015
BACKGROUND This study concerns the application of magnetic heteropolyacids composite for the adsorption of organic compounds from aqueous solutions. A novel magnetic Preyssler/chitosan/Fe3O4 nanoparticles composite (PCNF) adsorbent was designed using a one-pot synthesis method and characterized by multiple techniques including X-ray powder diffraction (XRD), infrared spectroscopy (FT-IR), N2 adsorption–desorption isotherms and scanning electron microscopy (SEM). RESULTS The adsorption activity of PCNF was evaluated by the removal of methyl orange (as an anionic azo dye model, MO) from aqueous solution and all the factors influencing the removal efficiency, such as adsorbent dosage, initial …
Volatile Compounds in Citrus Essential Oils: A Comprehensive Review
2019
[EN] The essential oil fraction obtained from the rind of Citrus spp. is rich in chemical compounds of interest for the food and perfume industries, and therefore has been extensively studied during the last decades. In this manuscript, we provide a comprehensive review of the volatile composition of this oil fraction and rind extracts for the 10 most studied Citrus species: C. sinensis (sweet orange), C. reticulata (mandarin), C. paradisi (grapefruit), C. grandis (pummelo), C. limon (lemon), C. medica (citron), C. aurantifolia (lime), C. aurantium (bitter orange), C. bergamia (bergamot orange), and C. junos (yuzu). Forty-nine volatile organic compounds have been reported in all 10 species,…
<title>Influence of strong static magnetic field on human cancer HT 1080 cells</title>
2001
The aim of this study was to investigate strong uniform magnetic field influence on the human cancer cells HT 1080. The cells were treated with magnetic field of intensity 1,16 Tesla and with anticancer agent - cis-platinum 0.025 mg/ml or vincristinum 2-3 ng/ml. The intact and the treated cell samples were incubated in a medium with acridine orange (AO). The magnetic field after 15 minutes of influence significantly increased cytoplasmic red fluorescence. Increased AO accumulation in lysosomes suggested to cancer cell metabolic activity stimulation.© (2001) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Toluidine Blue Test for Sperm DNA Integrity and Elaboration of Image Cytometry Algorithm
2003
Background: Sperm DNA integrity is of paramount importance in the prognosis of fertility. We applied image cytometry to a toluidine blue (TB) test we recently proposed. Methods: Sperm samples from 33 men were assayed for standard sperm parameters and classified as normal or abnormal. Sperm smears were subjected to the TB test, DNA denaturation testing with acridine orange (AO), and terminal deoxyuridine triphosphate biotin nick end labeling (TUNEL). In CCD image analysis, TB-stained sperm cell heads were microscopically assigned to one of four color groups (dark, blue, light violet, and light blue). The optical densities of 6,600 cells in green and red CCD images were used to elaborate an a…
A comparative study of photocatalytically active nanocrystalline tetragonal T zyrcon- type and monoclinic scheelite-type bismuth vanadate
2018
The authors from Vinča Institute of Nuclear Sciences acknowledge the financial support of the Ministry of Education, Science and Technological Development of the Republic of Serbia (Project no: 172056 ). The work of K. Smits was supported by Latvian National Research Program IMIS2 (Grant no. 302/2012 ).
Enhancing the photocatalytic properties of PbS QD solids: the ligand exchange approach.
2019
Surface engineering of nanomaterials is a promising tool towards the design of new materials for conversion of solar energy into chemical energy. In this work, we examine the influence of ligand exchange on the photocatalytic performance of solution-processed PbS films. We test different ligands such as oleylamine (OAm), 1,2-ethanedithiol (EDT), 3-mercaptopropionic acid (MPA) and tetrabutylammonium iodide (TBAI). The study demonstrates that PbS films capped with MPA and EDT exhibit 3.5-fold enhanced photocatalytic performance for the photodecomposition of methyl orange upon sunlight exposure. Both band energy alignment and charge carrier transport have a strong impact on the generation of r…
Au–NiOx nanocomposite for hot electron-assisted plasmonic photocatalysis
2020
Nanocomposites of metal–oxide semiconductors containing Au nanoparticles are emerging multifunctional materials for sunlight-driven highly efficient photocatalysis. Here we report a solution-processed nanocomposite synthesis of Au–NiOx nanocomposite films by slot-die coating and plotter printing in a straightforward in situ synthesis approach. Our study demonstrated a significantly enhanced photocatalytic decomposition reaction of MO upon exposure of Au–NiOx films to a sunlight simulator. The excellent photocatalytic activity is ascribed to the generation of hot carriers after LSPR excitation in Au NPs. Suitable band alignment between the valence band of NiOx and the Fermi level of Au NPs l…