Search results for " profili"
showing 10 items of 846 documents
The Role of Ancestral Duplicated Genes in Adaptation to Growth on Lactate, a Non-Fermentable Carbon Source for the Yeast Saccharomyces cerevisiae
2021
This article belongs to the Section Molecular Informatics.
PSD3 downregulation confers protection against fatty liver disease
2022
Fatty liver disease (FLD) is a growing health issue with burdening unmet clinical needs. FLD has a genetic component but, despite the common variants already identified, there is still a missing heritability component. Using a candidate gene approach, we identify a locus (rs71519934) at the Pleckstrin and Sec7 domain-containing 3 (PSD3) gene resulting in a leucine to threonine substitution at position 186 of the protein (L186T) that reduces susceptibility to the entire spectrum of FLD in individuals at risk. PSD3 downregulation by short interfering RNA reduces intracellular lipid content in primary human hepatocytes cultured in two and three dimensions, and in human and rodent hepatoma cell…
High-resolution genotyping of Campylobacter strains isolated from poultry and humans with amplified fragment length polymorphism fingerprinting.
1999
ABSTRACT For epidemiological studies of Campylobacter infections, molecular typing methods that can differentiate campylobacters at the strain level are needed. In this study we used a recently developed genotyping method, amplified fragment length polymorphism (AFLP), which is based on selective amplification of restriction fragments of chromosomal DNA, for genetic typing of Campylobacter jejuni and Campylobacter coli strains derived from humans and poultry. We developed an automated AFLP fingerprinting method in which restriction endonucleases Hin dIII and Hha I were used in combination with one set of selective PCR primers. This method resulted in evenly distributed band patterns for amp…
Comparative Transcriptomic Analysis Reveals Similarities and Dissimilarities in Saccharomyces cerevisiae Wine Strains Response to Nitrogen Availabili…
2015
Nitrogen levels in grape-juices are of major importance in winemaking ensuring adequate yeast growth and fermentation performance. Here we used a comparative transcriptome analysis to uncover wine yeasts responses to nitrogen availability during fermentation. Gene expression was assessed in three genetically and phenotypically divergent commercial wine strains (CEG, VL1 and QA23), under low (67 mg/L) and high nitrogen (670 mg/L) regimes, at three time points during fermentation (12 h, 24 h and 96 h). Two-way ANOVA analysis of each fermentation condition led to the identification of genes whose expression was dependent on strain, fermentation stage and on the interaction of both factors. The…
Evaluation of genotypic and phenotypic methods to distinguish clinical from environmental Vibrio vulnificus strains.
2009
ABSTRACT Vibrio vulnificus is a heterogeneous bacterial species that comprises virulent and avirulent strains from environmental and clinical sources that have been grouped into three biotypes. To validate the typing methods proposed to distinguish clinical from environmental isolates, we performed phenotypic (API 20E, API 20NE, and BIOLOG tests) and genetic (ribotyping and DNA polymorphism at several loci) studies with a large strain collection representing different biotypes, origins, and host ranges. No phenotypic method was useful for biotyping or grouping strains with regard to the origin of an isolate, and only the BIOLOG system was reliable for identifying the strains at the species …
DNA Fingerprinting for the Protection of Endangered Species
1991
The main application of DNA fingerprinting is based on identity and paternity testing in man [6, 7]. In the last years it has also been increasingly used for zoological problems [2–5, 8, 10, 12, 13]. In this study DNA fingerprinting was applied for “forensics” in the field of protection of endangered species.
SSR-based DNA fingerprints reveal the genetic diversity of Sicilian olive (Olea europaeaL.) germplasm
2005
Twelve published simple sequence repeat (SSR; microsatellite) markers, belonging to the ssrOeUA-DCA, GAPU and UDO series, were tested in a panel of 46 accessions of olive germplasm belonging to 30 unique cultivars collected in seven Provinces of Sicily. Four well-known reference olive cultivars were also added. The analysis was carried out on an automatic capillary sequencer using fluorescent dyes, and fragment sizes were determined using internal standards. The results allowed us to rank the SSRs assayed according to their information content and reproducibility. Up to 115 alleles were identified (119, if those unique to sport mutations were included), the frequency of which allowed geneti…
Emotional and motivational profiles in the giftedness
2020
L’articolo bersaglio di Cornoldi (2020) dal titolo: «Teorie dell’intelligenza e approcci allo studio della plusdotazione» offre l’opportunità di approfondire la discussione su una tematica che è stata negli ultimi anni oggetto di rinnovato interesse da parte sia della comunità scientifica sia delle istituzioni scolastiche. Basti pensare, da una parte, all’incremento nella produzione scientifica di ricerche volte a delineare il profilo degli studenti plusdotati o ad alto potenziale, dall’altro alla nota MIUR «Alunni con bisogni educativi speciali. Chiarimenti» del 3 aprile 2019 che esplicita le misure, già adottate da molte istituzioni scolastiche in linea con la Direttiva del 27 dicembre 20…
Giudizio abbreviato
2015
Il rito, ammesso dal giudice su richiesta del solo imputato, attribuisce a quest’ultimo un vero e proprio diritto a rinunciare alle garanzie dibattimentali proprie del giudizio ordinario in cambio dell’effetto premiale previsto in caso di condanna. Il contributo ha un approccio critico mettendo in risalto, dopo una breve storia dell'istituto, le novità introdotte dal legislatore del 1999.
Rapid adaptation of signaling networks in the fungal pathogen Magnaporthe oryzae
2019
Abstract Background One fundamental question in biology is how the evolution of eukaryotic signaling networks has taken place. “Loss of function” (lof) mutants from components of the high osmolarity glycerol (HOG) signaling pathway in the filamentous fungus Magnaporthe oryzae are viable, but impaired in osmoregulation. Results After long-term cultivation upon high osmolarity, stable individuals with reestablished osmoregulation capacity arise independently from each of the mutants with inactivated HOG pathway. This phenomenon is extremely reproducible and occurs only in osmosensitive mutants related to the HOG pathway – not in other osmosensitive Magnaporthe mutants. The major compatible so…