Search results for " profiling"
showing 10 items of 826 documents
von Hippel-Lindau Protein-Mediated Repression of Tumor Necrosis Factor Alpha Translation Revealed through Use of cDNA Arrays
2003
Based on evidence that the von Hippel-Lindau (VHL) tumor suppressor protein is associated with polysomes and interacts with translation regulatory factors, we set out to investigate the potential influence of pVHL on protein translation. To this end, renal cell carcinoma (RCC) cells that either lacked pVHL or expressed pVHL through stable transfection were used to prepare RNA from cytosolic (unbound) and polysome-bound fractions. Hybridization of cDNA arrays using RNA from each fraction revealed a subset of transcripts whose abundance in polysomes decreased when pVHL function was restored. The tumor necrosis factor alpha (TNF-alpha) mRNA was identified as one of the transcripts that prefere…
NOSIP, a novel modulator of endothelial nitric oxide synthase activity.
2001
Production of nitric oxide (NO) in endothelial cells is regulated by direct interactions of endothelial nitric oxide synthase (eNOS) with effector proteins such as Ca2+-calmodulin, by posttranslational modifications such as phosphorylation via protein kinase B, and by translocation of the enzyme from the plasma membrane caveolae to intracellular compartments. Reversible acylation of eNOS is thought to contribute to the intracellular trafficking of the enzyme; however, protein factor(s) that govern the translocation of the enzyme are still unknown. Here we have used the yeast two-hybrid system and identified a novel 34 kDa protein, termed NOSIP (eNOS interacting protein), which avidly binds …
Human Papilloma Virus-Dependent HMGA1 Expression Is a Relevant Step in Cervical Carcinogenesis
2008
HMGA1 is a member of a small family of architectural transcription factors involved in the coordinate assembly of multiprotein complexes referred to as enhanceosomes. In addition to their role in cell proliferation, differentiation, and development, high-mobility group proteins of the A type (HMGA) family members behave as transforming protoncogenes either in vitro or in animal models. Recent reports indicated that HMGA1 might counteract p53 pathway and provided an interesting hint on the mechanisms determining HMGA's transforming potential. HMGA1 expression is deregulated in a very large array of human tumors, including cervical cancer, but very limited information is available on the mole…
Discovering Homophily in Online Social Networks
2018
During the last ten years, Online Social Networks (OSNs) have increased their popularity by becoming part of the real life of users. Despite their tremendous widespread, OSNs have introduced several privacy issues as a consequence of the nature of the information involved in these services. Indeed, the huge amount of private information produced by users of current OSNs expose the users to a number of risks. The analysis of the users’ similarity in OSNs is attracting the attention of researchers because of its implications on privacy and social marketing. In particular, the homophily between users could be used to reveal important characteristics that users would like to keep hidden, hence …
Transcriptional Upregulation of Genes Related to Virulence Activation in Entamoeba histolytica
2007
Background To understand the molecular basis of virulence variability in Entamoeba histolytica , this study presents results about differential gene expression induced by E. histolytica trophozoites in liver of hamsters in order to produce experimental amebic liver abscess (ALA) and consequently reactivate its virulence. Methods Amebic cultures were studied before (BALA) and after (AALA) inoculation in hamster peritoneal cavity. Markers of pathogenicity such as the rate of erythrophagocytosis, hemolytic activity, and cytotoxic effects on MDCK cell monolayers were evaluated in order to correlate these phenotypic characteristics to differential gene expression between virulent and non-virulen…
A sequence motif enriched in regions bound by the Drosophila dosage compensation complex
2010
Abstract Background In Drosophila melanogaster, dosage compensation is mediated by the action of the dosage compensation complex (DCC). How the DCC recognizes the fly X chromosome is still poorly understood. Characteristic sequence signatures at all DCC binding sites have not hitherto been found. Results In this study, we compare the known binding sites of the DCC with oligonucleotide profiles that measure the specificity of the sequences of the D. melanogaster X chromosome. We show that the X chromosome regions bound by the DCC are enriched for a particular type of short, repetitive sequences. Their distribution suggests that these sequences contribute to chromosome recognition, the genera…
Global patterns of sequence evolution in Drosophila.
2007
This article is available from: http://www.biomedcentral.com/1471-2164/8/408
Immunomodulatory effects of dietary β-1,3-glucan from Euglena gracilis in rainbow trout (Oncorhynchus mykiss) immersion vaccinated against Yersinia r…
2012
Abstract Potential immunostimulatory effects of orally administered β-glucan were investigated in combination with immersion vaccination against enteric redmouth disease caused by Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss). A linear, unbranched and pure (purity ≥98%) β-1,3-glucan (syn. paramylon) from the alga Euglena gracilis was applied at an inclusion level of 1% β-glucan in feed administered at a rate of 1% biomass day−1 for 84 consecutive days. Fish were vaccinated after two weeks of experimental feeding and bath challenged with live Y. ruckeri six weeks post-vaccination. Blood and head kidney were sampled at day 0, 13 (1 day pre-vaccination), 15, 55, 59 (day 3 post-challe…
Semantic User Profiling for Digital Advertising
2015
International audience; With the emergence of real-time distribution of online advertising space (“real-time bidding”), user profiling from web navigation traces becomes crucial. Indeed, it allows online advertisers to target customers without interfering with their activities. Current techniques apply traditional methods as statistics and machine learning, but suffer from their limitations. As an answer, the proposed approach aims to develop and evaluate a semantic-based user profiling system for digital advertising.
Customizing Semantic Profiling for Digital Advertising
2014
International audience; Personalization is the new magic buzzword of application development. To make the complexity of today's application functionalities and information spaces "digestible", customization has become the new go-to technique. But while those technologies aim to ease the consumption of media for their users, they suffer from the same problematic: in the age of Big Data, applications have to cope with a conundrum of heterogeneous information sources that have to be perceived, processed and interpreted. Researchers tend to aim for a maximum degree of integration to create the perfect, all-embracing personalization. The results are wide-range, but overly complex systems that su…