Search results for " rese"
showing 10 items of 14841 documents
Rac1 and PAK1 are upstream of IKK-ε and TBK-1 in the viral activation of interferon regulatory factor-3
2004
The anti-viral type I interferon (IFN) response is initiated by the immediate induction of IFN beta, which is mainly controlled by the IFN-regulatory factor-3 (IRF-3). The signaling pathways mediating viral IRF-3 activation are only poorly defined. We show that the Rho GTPase Rac1 is activated upon virus infection and controls IRF-3 phosphorylation and activity. Inhibition of Rac1 leads to reduced IFN beta promoter activity and to enhanced virus production. As a downstream mediator of Rac signaling towards IRF-3, we have identified the kinase p21-activated kinase (PAK1). Furthermore, both Rac1 and PAK1 regulate the recently described IRF-3 activators, I kappa B kinase- and TANK-binding kina…
Rho GTPases in human breast tumours: expression and mutation analyses and correlation with clinical parameters
2002
In the present study, we addressed the question of a putative relevance of Rho proteins in tumour progression by analysing their expression on protein and mRNA level in breast tumours. We show that the level of RhoA, RhoB, Rac1 and Cdc42 protein is largely enhanced in all tumour samples analysed (n=15) as compared to normal tissues originating from the same individual. The same is true for 32P-ADP-ribosylation of Rho proteins which is catalysed by Clostridium botulinum exoenzyme C3. Also the amount of Rho-GDI and ERK2 as well as the level of overall 32P-GTP binding acvitity was tumour-specific elevated, yet to a lower extent than Rho proteins. Although the amount of Rho proteins was enhance…
Molecular Mechanisms of the Blockage of Glioblastoma Motility
2021
Glioblastoma (GBM) is the most common and lethal brain tumor. GBM has a remarkable degree of motility and is able to infiltrate the healthy brain. In order to perform a rationale-based drug-repositioning study, we have used known inhibitors of two small Rho GTPases, Rac1 and Cdc42, which are upregulated in GBM and are involved in the signaling processes underlying the orchestration of the cytoskeleton and cellular motility. The selected inhibitors (R-ketorolac and ML141 for Cdc42 and R-ketorolac and EHT 1864 for Rac1) have been successfully employed to reduce the infiltration propensity of GBM in live cell imaging studies. Complementarily, all-atom simulations have elucidated the molecular …
The Co-Design of a Counter-Narrative Social Campaign: Second Generation Youths Against Radicalization
2021
This paper deals with the OLTRE project (ISF - DG Migration and Home Affairs, EU) funded for preventing the radicalization of the second-generation of migrants in Italy. This essay aims to study the production of an online communication campaign co-designed by second-generation youths. The four Universities engaged in the project, in order to collect the issues for the campaign made an in-depth sociological research and an interdisciplinary social network analysis. We will present the results of the non-standard field research. Starting from the different dimensions of the risk of radicalization proposed by the kaleidoscopic overview of risk factors (Sieckelinck and Gielen 2018: 5; Ranstorp…
Value of Combined PET Imaging with [18F]FDG and [68Ga]Ga-PSMA-11 in mCRPC Patients with Worsening Disease during [177Lu]Lu-PSMA-617 RLT
2021
Despite the promising results of prostate-specific membrane antigen (PSMA)-targeted radioligand therapy (RLT) in metastatic castration-resistant prostate cancer (mCRPC), some patients show worsening disease during PSMA-RLT. We investigated the value of combined [18F]FDG and [68Ga]Ga-PSMA-11 PET imaging in this setting. In n = 29 mCRPC patients with worsening disease after a median of four cycles of [177Lu]Lu-PSMA-617 RLT, combined [18F]FDG and [68Ga]Ga-PSMA-11 PET imaging was performed to detect [18F]FDG-avid lesions with low or no PSMA expression (mismatch lesions). To evaluate prognostic implication of mismatch, survival analyses regarding presence, location, and [18F]FDG PET-derived para…
Video aktivitāšu veidošana skolēnu iesaistīšanai angļu valodas stundās vidusskolā
2018
Skolotāja pieredzes darba tēma ir ‘Video aktivitāšu veidošana sklēnu iesaistīšanai angļu valodas stundās vidusskolā’. Darba autore strādā Gulbenes 2.vidusskolā par angļu valodas skolotāju piecpadsmit gadus. Situācija mūsdienās pierada ka digitālas tehnoloģijas ir nepārprotāmi ietekmējušās mūsu ikdienu. Cilvēki lieto digittālas ierīces visur, kad ir vajadzīgs un visur kur ir iespējams. Tēma ļoti saistīta ar šīm izmaiņam un ar nepieciešamību veidot stundas patīkāmākas, motivējošākās un effektivākās – gan skolotājiem gan skolēniem, ka resultātā skolēni būs iesaistīti mācību procesā pilnībā. Video skatīšanās nav tikai izklaide un laika pavdīšana stundās. Tas ietekmē izglītības procesu, jo tas a…
From Mobile Crimes to Crimes of Mobility
2020
Piipponen, Mäntymäki and Rodi-Risberg suggest that many contemporary crime narratives across the globe host a heightened interest in diverse and ambiguous mobilities, border crossings and borderlands. They propose that such mobilities and crossings reflect on recent sociocultural developments on local and global levels and communicate specific geopolitical anxieties. They position their own mobilities research perspective within existing crime fiction scholarship, especially within the so-called transnational and spatial turns. Introducing some key observations of mobilities research, they suggest that mobility can be considered both as an object of study in its own right and a critical len…
Rationale and design of the DARWIN-T2D (DApagliflozin Real World evIdeNce in Type 2 Diabetes): A multicenter retrospective nationwide Italian study a…
2017
Background Randomized controlled trials (RCTs) in the field of diabetes have limitations inherent to the fact that design, setting, and patient characteristics may be poorly transferrable to clinical practice. Thus, evidence from studies using routinely accumulated clinical data are increasingly valued. Aims We herein describe rationale and design of the DARWIN-T2D (DApagliflozin Real World evIdeNce in Type 2 Diabetes), a multicenter retrospective nationwide study conducted at 50 specialist outpatient clinics in Italy and promoted by the Italian Diabetes Society. Data synthesis The primary objective of the study is to describe the baseline clinical characteristics (particularly HbA1c) of pa…
Enhanced Customer Cooperation : Experiences with cooperative compliance in Finland
2018
This report examines the experiences with a collaborative compliance project – Enhanced Customer Cooperation (ECC) – introduced by the Finnish Tax Administration. The ECC was introduced by the Large Taxpayers’ Unit of the Finnish Tax Administration at the beginning of 2013, and it ran as a pilot until the end of 2015. Since the start of 2016, the ECC has been a part of the permanent operations of the Large Taxpayers’ Unit. Based on the interviews with tax officers, corporations participating in the ECC and tax lawyers and tax consultants, the ECC is bringing about a cultural change in the administrative practices and ways of communicating between tax authorities and taxpayers. In general, t…
Registered reports for qualitative research.
2022
Researchers are disincentivized from conducting urgently needed qualitative research, argues Veli-Matti Karhulahti. He recommends the adoption of registered reports for qualitative research as a remedial course of action. nonPeerReviewed