Search results for " secondary"

Deciphering metal-induced oxidative damages on glycated albumin structure and function

2014

Background: Metal ions such as copper or zinc are involved in the development of neurodegenerative pathologies and metabolic diseases such as diabetes mellitus. Albumin structure and functions are impaired following metal-and glucose-mediated oxidative alterations. The aim of this study was to elucidate effects of Cu(II) and Zn(II) ions on glucose-induced modifications in albumin by focusing on glycation, aggregation, oxidation and functional aspects. Methods: Aggregation and conformational changes in albumin were monitored by spectroscopy, fluorescence and microscopy techniques. Biochemical assays such as carbonyl, thiol groups, albumin-bound Cu, fructosamine and amine group measurements w…

Glycation alters ligand binding, enzymatic, and pharmacological properties of human albumin.

2015

Albumin, the major circulating protein in blood plasma, can be subjected to an increased level of glycation in a diabetic context. Albumin exerts crucial pharmacological activities through its drug binding capacity, i.e., ketoprofen, and via its esterase-like activity, allowing the conversion of prodrugs into active drugs. In this study, the impact of the glucose-mediated glycation on the pharmacological and biochemical properties of human albumin was investigated. Aggregation product levels and the redox state were quantified to assess the impact of glycation-mediated changes on the structural properties of albumin. Glucose-mediated changes in ketoprofen binding properties and esterase-lik…

Thermal aggregation of glycated bovine serum albumin

2010

International audience; Aggregation and glycation processes in proteins have a particular interest in medicine fields and in food technology. Serum albumins are model proteins which are able to self-assembly in aggregates and also sensitive to a non-enzymatic glycation in cases of diabetes. In this work, we firstly reported a study on the glycation and oxidation effects on the structure of bovine serum albumin (BSA). The experimental approach is based on the study of conformational changes of BSA at secondary and tertiary structures by FTIR absorption and fluorescence spectroscopy, respectively. Secondly, we analysed the thermal aggregation process on BSA glycated with different glucose con…

The inhibition of glycerol permeation through aquaglyceroporin-3 induced by mercury(II)

2016

Mercurial compounds are known to inhibit water permeation through aquaporins (AQPs). Although in the last years some hypotheses were proposed, the exact mechanism of inhibition is still an open question and even less is known about the inhibition of the glycerol permeation through aquaglyceroporins. Molecular dynamics (MD) simulations of human aquaporin-3 (AQP3) have been performed up to 200 ns in the presence of Hg2+ ions. For the first time, we have observed the unbiased passage of a glycerol molecule from the extracellular to cytosolic side. Moreover, the presence of Hg2+ ions covalently bound to Cys40 leads to a collapse of the aromatic/arginine selectivity filter (ar/R SF), blocking th…

Membrane-insertion fragments of Bcl-xL, Bax, and Bid.

2004

Apoptosis regulators of the Bcl-2 family associate with intracellular membranes from mitochondria and the endoplasmic reticulum, where they perform their function. The activity of these proteins is related to the release of apoptogenic factors, sequestered in the mitochondria, to the cytoplasm, probably through the formation of ion and/or protein transport channels. Most of these proteins contain a C-terminal putative transmembrane (TM) fragment and a pair of hydrophobic alpha helices (alpha5-alpha6) similar to the membrane insertion fragments of the ion-channel domain of diphtheria toxin and colicins. Here, we report on the membrane-insertion properties of different segments from antiapopt…

Soils with Gypsic horizon in southern Sicily, Italy

2005

Lower secondary school pupils’ written descriptions of their experiences with bullying and the tendency to seek help

2021

The purpose of this study was to examine lower secondary school students’ (n = 223) descriptions of bullying and their experiences with the phenomenon. Students at three schools in Norway completed a survey containing open-ended questions. We employed a mixed-methods analytical approach in which responses were coded thematically and then quantified. Findings indicate that students viewed bullying in a manner consistent with prevalent definitions in the literature. However, they were often unsure whether social conflicts they had experienced would be considered bullying. School was clearly featured as the primary arena for bullying, although bullying in free-time activities and on social med…

Hardy spaces and quasiconformal maps in the Heisenberg group

2023

We define Hardy spaces $H^p$, $00$ such that every $K$-quasiconformal map $f:B \to f(B) \subset \mathbb{H}^1$ belongs to $H^p$ for all $0<p<p_0(K)$. Second, we give two equivalent conditions for the $H^p$ membership of a quasiconformal map $f$, one in terms of the radial limits of $f$, and one using a nontangential maximal function of $f$. As an application, we characterize Carleson measures on $B$ via integral inequalities for quasiconformal mappings on $B$ and their radial limits. Our paper thus extends results by Astala and Koskela, Jerison and Weitsman, Nolder, and Zinsmeister, from $\mathbb{R}^n$ to $\mathbb{H}^1$. A crucial difference between the proofs in $\mathbb{R}^n$ and $\mathbb{…

Promoting coordination in Norwegian health care

2011

This is the journal's version originally published in International Journal of Integrated Care. This article is designet as ”Open Access”. © International Journal of Integrated Care: http://www.ijic.org/index.php/ijic/index Introduction: The Norwegian health care system is well organized within its two main sectors - primary health and long term care on the one hand, and hospitals and specialist services on the other. However, the relation between them lacks mediating structures. Policy practice: Enhancing coordination between primary and secondary health care has been central in Norwegian health care policy the last decade. In 2003 a committee was appointed to identify coordination problem…

A novel RNA-binding motif in influenza A virus non-structural protein 1.

1997

The solution NMR structure of the RNA-binding domain from influenza virus non-structural protein 1 exhibits a novel dimeric six-helical protein fold. Distributions of basic residues and conserved salt bridges of dimeric NS1(1-73) suggest that the face containing antiparallel helices 2 and 2′ forms a novel arginine-rich nucleic acid binding motif.