Search results for " secondary"
showing 10 items of 692 documents
Cholesterol facilitates interactions between α‐synuclein oligomers and charge‐neutral membranes
2015
AbstractOligomeric species formed during α-synuclein fibrillation are suggested to be membrane-disrupting agents, and have been associated with cytotoxicity in Parkinson’s disease. The majority of studies, however, have revealed that the effect of α-synuclein oligomers is only noticeable on systems composed of anionic lipids, while the more physiologically relevant zwitterionic lipids remain intact. We present experimental evidence for significant morphological changes in zwitterionic membranes containing cholesterol, induced by α-synuclein oligomers. Depending on the lipid composition, model membranes are either unperturbed, disrupt, or undergo dramatic morphological changes and segregate …
Self-assembly of 3,5-bis(ethoxycarbonyl)pyrazolate anions and ammonium cations of β-phenylethylamine or homoveratrylamine into hetero-double-stranded…
2009
Hydrogen-bonded double-stranded hetero-helices are formed when reacting sodium 3,5-bis(ethoxycarbonyl)pyrazolate with beta-phenethylammonium or homoveratrylammonium chloride, in which one of the strands is defined by the ammonium cations and the other one by the pyrazolate anions.
Characterization of liver cytokeratin as a major target antigen of anti-SLA antibodies.
1990
Abstract Anti-SLA antibodies characterize a newly defined subgroup of patients with autoimmune chronic active hepatitis. The aim of the present study was the immunochemical characterization of the target antigen(s) of anti-SLA antibodies. Anti-SLA-positive sera were found to contain high titres of anti-cytokeratin antibodies. In immunoblotting analyses with 100 000 × g supernatants of human liver homogenates (S-100) these sera recognized various proteins with a molecular mass of 40–60 kDa. These proteins were also recognized by monoclonal anti-cytokeratin antibodies. Two-dimensional co-electrophoresis and immunoblotting analysis of S-100 and liver cytokeratins showed that anti-SLA antibodie…
Polyclonal antibodies to mannan from yeast also recognize the carbohydrate structure of gp120 of the AIDS virus: an approach to raise neutralizing an…
1990
This study initiates a new method of developing an antigen which might be useful in the prevention of HIV-1 infection. Using a mannan preparation from Saccharomyces cerevisiae neutralizing antiserum was raised in rabbits which prevents HIV-1 infection in vitro up to a titre of 1:128. The corresponding antibody preparation neutralized the in vitro infectivity down to a concentration of 5 micrograms/ml. Analytical studies suggest that the antibodies are directed against the mannose residues of the HIV-1 glycoprotein (gp) 120 and its precursor gp 160.
Interaction of theEscherichia colitransporter DctA with the sensor kinase DcuS: presence of functional DctA/DcuS sensor units
2012
The aerobic Escherichia coli C(4) -dicarboxylate transporter DctA and the anaerobic fumarate/succinate antiporter DcuB function as obligate co-sensors of the fumarate responsive sensor kinase DcuS under aerobic or anaerobic conditions respectively. Overproduction under anaerobic conditions allowed DctA to replace DcuB in co-sensing, indicating their functional equivalence in this capacity. In vivo interaction studies between DctA and DcuS using FRET or a bacterial two-hybrid system (BACTH) demonstrated their interaction. DctA-YFP bound to an affinity column and was able to retain DcuS. DctA shows substantial sequence and secondary structure conservation to Glt(Ph), the Na(+)/glutamate sympo…
Student evaluations of the credibility and argumentation of online sources
2021
This study investigated upper secondary school students’ skills in evaluating the credibility and argumentative content of a blog text and a YouTube video. Both sources concerned child vaccination, the blog text opposing and the YouTube video supporting it. Students rated each source as credible, fairly credible or non-credible, justified their ratings, and analyzed the argumentation of both sources. Their justifications were analyzed for trustworthiness and expertise and their argument analyses for identification of the main position of the source and the reasons supporting it. Students’ justification skills proved fairly weak, and they also struggled with recognizing unbalanced argumentat…
Life Cycle Assessment of Asphalt Mixtures with WMR
2023
This study aimed at providing pavement specialists with international average values of greenhouse gas emissions (GHG) and energy consumption related to the manufacturing of asphalt mixtures (cradle-to-gate), with and without waste and secondary materials for roads (WMRs). In order to achieve this goal, TG leaders first referred to current standards to define a methodology for obtaining benchmark values according to ISO 21678:2020. Then, a data collection tool was designed and provided to a group of international partners to build a life cycle inventory for asphalt mixtures including data from materials up to plant manufacturing (A1–A3). Partners were asked to provide information for any ty…
Study on ambient concentrations of PM10, PM10-2.5, PM2.5 and gaseous pollutants. Trace elements and chemical speciation of atmospheric particulates
2010
Abstract This study provides the first comprehensive report on mass concentrations of particulate matter of various sizes, inorganic and organic gas concentrations monitored at three sampling sites in the city of Palermo (Sicily, Italy). It also provides information on the water-soluble species and trace elements. A total of 2054 PM10 (1333) and PM2.5 (721) daily measurements were collected from November 2006 to February 2008. The highest mass concentrations were observed at the urban stations, average values being about two times higher than those at the suburban (control) site. Time variations in PM10 and also PM10–2.5 were observed at the urban stations, the highest concentrations being …
Sylvén, L. K. (2019). Investigating Content and Language Integrated Learning. Insights from Swedish High Schools. Bristol, UK: Multilingual Matters
2019
This volume presents a three-year research project on Content and Language Integration in Swedish Schools (CLISS). CLISS particularly focused on sciences and economics programs in senior high schools (Grades 10-12; 245 participants), which intended to prepare students for higher education. Divided into five sections, the book explores the language, rather than content, side of CLIL. The volume starts with background information concerning CLISS and CLIL in Sweden. The following four sections of the book concern assessment and motivation (Chapters 4-5); English vocabulary, reading comprehension and exposure to English (Chapters 6-9); students’ L1 proficiency and development through CLIL (Cha…
A Derivation of the Vlasov-Stokes System for Aerosol Flows from the Kinetic Theory of Binary Gas Mixtures
2016
In this short paper, we formally derive the thin spray equation for a steady Stokes gas, i.e. the equation consists in a coupling between a kinetic (Vlasov type) equation for the dispersed phase and a (steady) Stokes equation for the gas. Our starting point is a system of Boltzmann equations for a binary gas mixture. The derivation follows the procedure already outlined in [Bernard-Desvillettes-Golse-Ricci, arXiv:1608.00422 [math.AP]] where the evolution of the gas is governed by the Navier-Stokes equation.