Search results for " secretion"

showing 10 items of 490 documents

LaXp180, a mammalian ActA-binding protein, identified with the yeast two-hybrid system, co-localizes with intracellular Listeria monocytogenes

2001

The Listeria monocytogenes surface protein ActA is an important virulence factor required for listerial intracellular movement by inducing actin polymerization. The only host cell protein known that directly interacts with ActA is the phosphoprotein VASP, which binds to the central proline-rich repeat region of ActA. To identify additional ActA-binding proteins, we applied the yeast two-hybrid system to search for mouse proteins that interact with ActA. A mouse cDNA library was screened for ActA-interacting proteins (AIPs) using ActA from strain L. monocytogenes EGD as bait. Three different AIPs were identified, one of which was identical to the human protein LaXp180 (also called CC1). Bind…

Two-hybrid screeningImmunologyMolecular Sequence DataAutophagy-Related ProteinsFluorescent Antibody TechniqueStathminmacromolecular substancesmedicine.disease_causeMicrobiologylaw.inventionCell LineMicefluids and secretionsListeria monocytogenesBacterial ProteinslawVirologyTwo-Hybrid System TechniquesmedicineAnimalsHumansListeriosisAmino Acid SequencebiologyReverse Transcriptase Polymerase Chain ReactionBinding proteintechnology industry and agricultureIntracellular Signaling Peptides and ProteinsMembrane ProteinsProteinsListeria monocytogenesActinsBiochemistryPhosphoproteinembryonic structuresCOS CellsRecombinant DNAbiology.proteinbacteriaSignal transductionCarrier ProteinsIntracellularPlasmids
researchProduct

Effects of tetraethylammonium ions on frequency-dependent vasopressin release from the rat neurohypophysis.

1988

1. Isolated rat neurohypophyses were fixed by their stalks to a platinum wire electrode and superfused with oxygenated Krebs-HEPES solution. Vasopressin release into the medium was determined by radioimmunoassay. Vasopressin secretion was increased by electrical stimulation at different frequencies (3-30 Hz) and different train lengths (75-900 pulses). The effects of tetraethylammonium (TEA) ions and of enhanced calcium were tested. 2. Electrical stimulation at 7.5 or 15 Hz evoked a markedly larger release of vasopressin than stimulation at 3 Hz. During continuous stimulation at 7.5 and 15 Hz the evoked vasopressin release per pulse declined rapidly, but with similar time constants for both…

Vasopressinmedicine.medical_specialtyTime FactorsPhysiologychemistry.chemical_elementStimulationStimulus (physiology)CalciumIn Vitro Techniqueschemistry.chemical_compoundPituitary Gland PosteriorInternal medicinemedicineExtracellularAnimalsTetraethylammoniumChemistryTetraethylammoniumRadioimmunoassayRats Inbred StrainsTetraethylammonium CompoundsElectric StimulationRatsArginine VasopressinKineticsEndocrinologyVasopressin secretionCalciumFemaleResearch ArticleThe Journal of physiology
researchProduct

Variation in somatic cell count, California mastitis test, and electrical conductivity among various fractions of ewe's milk.

1991

Abstract Variation of three estimates of udder inflammation (SCC, California mastitis test, and electrical conductivity) among the foremilk, machine milk, and hand stripping samples were studied. Foremilk and hand stripping milks were taken from each individual teat; machine milk was taken from the entire udder. For this experiment a total of 30 Manchega ewes were subjected to machine milking during the 9th wk of lactation. Samples were taken in duplicate during two milkings on consecutive mornings. Significant differences were observed in the SCC and electrical conductivity, but not in California mastitis test, between foremilk and stripping milk The stripping fraction had higher SCC (70 ±…

Veterinary medicineStripping (chemistry)Sheep DiseasesCell CountMastitisMilkingfluids and secretionsElectrical resistivity and conductivityLactationGeneticsmedicineAnimalsUdderSheepChemistryElectric Conductivityfood and beveragesmedicine.diseaseMastitismedicine.anatomical_structureMilkAnimal Science and ZoologyFemaleSomatic cell countFood ScienceCalifornia mastitis testJournal of dairy science
researchProduct

Relative Frequencies and Significance of Faecal Coliforms as Indicators Related to Water Temperature

1983

Summary The faecal coliforms at different sites of a hypereutrophic lake near Valencia (Albufera) were identified and their relative amounts established along an annual cycle. Using lauryl tryptose broth at 35°C, followed by incubation at 44.4°C in 2% brilliant green bile, Escherichia coli and Klebsiella pneumoniae are practically the only coliforms present. A positive correlation was found between the water temperature and the relative amount of these two coliforms: K. pneumoniae predominates at high water temperatures, whereas E. coli shows preponderance during the cold period. The role of K. pneumoniae as the only faecal indicator under the circumstances described in the work is emphasiz…

Veterinary medicinebiologyKlebsiella pneumoniaeLauryl tryptose brothGeneral Medicineequipment and suppliesmedicine.disease_causebiology.organism_classificationMicrobiologyFecal coliformchemistry.chemical_compoundfluids and secretionschemistryBrilliant greenWater temperaturemedicineCold periodGeneral Agricultural and Biological SciencesEscherichia coliIncubationZentralblatt für Mikrobiologie
researchProduct

Antioxidant capacity of cow milk, whey and deproteinized milk

2009

The total antioxidant capacity (hydrophilic plus lipophilic) of sixteen different commercial samples of pasteurized and ultra high temperature (UHT) treated milk was determined using the oxygen radical absorbance capacity assay using fluorescein as a fluorescent probe. A significant correlation between the percentage of fat and the value of the total antioxidant capacity was found in milk samples obtained from the same batch of raw milk. Analyses of the whole milk, whey and deproteinized milk showed that the major contributor to the total antioxidant capacity of whole milk was the casein fractions, while albumin was the major contributor to the total antioxidant capacity of whey protein. Hy…

Whey proteinAntioxidantVitamin COxygen radical absorbance capacityChemistrymedicine.medical_treatmentfood and beveragesPasteurizationantioxidant capacity cow milkRaw milkAscorbic acidApplied Microbiology and Biotechnologylaw.inventionfluids and secretionslawCaseinmedicineFood scienceFood ScienceInternational Dairy Journal
researchProduct

The foaming properties of camel and bovine whey: The impact of pH and heat treatment

2018

International audience; he effect of heat treatment (70 degrees C or 90 degrees C for 30 min) on the foaming and interfacial properties of acid and sweet whey obtained from bovine and camel fresh milk was examined. The maximum foamability and foam stability were observed for acid whey when compared to sweet whey for both milks, with higher values for the camel whey. This behavior for acid whey was explained by the proximity of the pI of whey protein (4.9-5.2), where proteins were found to carry the lowest negative charge as confirmed by the zeta potential measurements. Interfacial properties of acid camel whey and acid bovine whey were preserved at air water interface even after a heat trea…

Whey proteinHot TemperatureAir water interfaceCamel and bovine wheyAnalytical Chemistryfluids and secretions[SDV.IDA]Life Sciences [q-bio]/Food engineeringZeta potentialmixed layersFood scienceBeta-lactoglobulinbiologybeta-lactoglobulinChemistrypHdigestive oral and skin physiology[ SDV.IDA ] Life Sciences [q-bio]/Food engineeringaggregationfood and beverages04 agricultural and veterinary sciencesGeneral MedicineHydrogen-Ion Concentration040401 food science[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM]lactoferrinmilk-proteinsendocrine systemCamelusanimal structuresHeat treatmentinterfacesFresh milk0404 agricultural biotechnologyWheyNegative chargeFoaming propertiesalpha-lactalbuminAnimals[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM]adsorption behaviorChromatographydromedarius milkViscoelastic modulus0402 animal and dairy sciencestability040201 dairy & animal scienceWhey ProteinsAlpha-lactalbuminbiology.proteinCattle[SDV.AEN]Life Sciences [q-bio]/Food and NutritionFood Science
researchProduct

Localization of the tannin-binding site on the salivary PRP IB5

2014

Localization of the tannin-binding site on the salivary PRP IB5. 10. European Symposium on Saliva

[ SDV.BV ] Life Sciences [q-bio]/Vegetal Biologyfluids and secretionsstomatognathic systemeducation[SDV.BV]Life Sciences [q-bio]/Vegetal Biology[SDV.BV] Life Sciences [q-bio]/Vegetal Biologyhealth care economics and organizationshumanities
researchProduct

Influence of type III bacterial secretion system on the interactions between plant and non pathogenic fluorescent Pseudomonads spp.

2010

No abstract

[SDE] Environmental Sciences[ SDV.BV ] Life Sciences [q-bio]/Vegetal BiologyInteractions plantes-microorganismes bénéfiques[SDV] Life Sciences [q-bio]Système de sécrétion de type IIIChampignons mycorhizogènes à arbusculesMycorrhiza helper bacteria (MHB)Medicago truncatula[SDV.BV]Life Sciences [q-bio]/Vegetal Biology[SDV.BV] Life Sciences [q-bio]/Vegetal BiologytheseRELATION PLANTE-MICROORGANISMESYSTEME DE SECRETIONPseudomonas spp. fluorescents
researchProduct

Infant fat acceptance: role of milk feeding practices and links with saliva composition

2017

Infant fat acceptance: role of milk feeding practices and links with saliva composition. Annual meeting of the Society for the Study of Ingestive Behavior (SSIB)

[SDV.AEN] Life Sciences [q-bio]/Food and Nutritionfluids and secretions[ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
researchProduct

Salivary lipolysis Infant’s saliva lipolysis: relationships with age, milk and complementary feeding history

2017

Salivary lipolysis Infant’s saliva lipolysis: relationships with age, milk and complementary feeding history. 5. international conference on food digestion

[SDV.AEN] Life Sciences [q-bio]/Food and Nutritionfluids and secretions[ SDV.AEN ] Life Sciences [q-bio]/Food and Nutritioneducation[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
researchProduct