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showing 10 items of 1641 documents
α-Aminoalkylphosphonates as a tool in experimental optimisation of P1 side chain shape of potential inhibitors in S1 pocket of leucine- and neutral a…
2005
Abstract The synthesis and biological activity studies of the series of structurally different α-aminoalkylphosphonates were performed in order to optimise the shape of the side chain of the potential inhibitors in S1 pocket of leucine aminopeptidase [E.C.3.4.11.1]. Analysis of a series of compounds with aromatic, aliphatic and alicyclic P1 side chains enabled to find out the structural features, optimal for that fragment of inhibitors of LAP. The most active among all investigated compounds were the phosphonic analogues of homo-tyrosine ( K i = 120 nM) and homo-phenylalanine ( K i = 140 nM), which even as racemic mixtures were better inhibitors in comparison with the best till now-phosph…
A measuring instrument of the audience of a web site : the network analysis
1999
Marketing on the Internet upsets the traditional commercial techniques. To optimize the audience of its site, even its sales on this channel, a company needs relevant statistical data. The aim of our research is to renew the approach of Log files analyzers currently available, by showing the contributions of network analysis
Procollagen C-proteinase Enhancer Stimulates Procollagen Processing by Binding to the C-propeptide Region Only*
2011
Background: Procollagen C-proteinase enhancer-1 (PCPE-1) is an extracellular glycoprotein that increases activity of certain zinc metalloproteinases involved in tissue development and repair. Results: PCPE-1 binds uniquely to the C-propeptide region of the procollagen molecule. Conclusion: PCPE-1 enhances proteolysis by binding solely to the procollagen C-propeptides. Significance: These data may lead to future applications in the development of antifibrotic therapies.
Context-dependent Pax-5 repression of a PU.1/NF-κB regulated reporter gene in B lineage cells
2001
Enhancers located in the 3' end of the locus in part regulate immunoglobulin heavy chain (IgH) gene expression. One of these enhancers, HS 1,2, is developmentally regulated by DNA binding proteins like NF-kappaB, Pax-5 and the protein complex NF-alphaP in B lineage cells. Here we report that NF-alphaP is the ets protein PU.1. A glutathione-S-transferase (GST)-pulldown assay demonstrated that PU.1 can physically interact with NF-kappaB in solution. Experiments in COS cells showed that PU.1 and NF-kappaB (p50/c-Rel) can activate transcription of an enhancer linked reporter gene. The paired domain protein Pax-5 has previously been shown to repress enhancer-dependent transcription. Additional c…
The Sea Urchin sns Insulator Blocks CMV Enhancer following Integration in Human Cells
2001
Insulators are a new class of genetic elements that attenuate enhancer function directionally. Previously, we characterized in sea urchin a 265-bp-long insulator, termed sns. To test insulator activity following stable integration in human cells, we placed sns between the CMV enhancer and a tk promoter up-stream of a GFP transgene of plasmid or retroviral vectors. In contrast to controls, cells transfected or transduced with insulated constructs displayed a barely detectable fluorescence. Southern blot and PCR ruled out vector rearrangement following integration into host DNA; RNase protection confirmed the enhancer blocking activity. Finally, we demonstrate that two cis-acting sequences, p…
Polycationic Monomeric and Homodimeric Asymmetric Monomethine Cyanine Dyes with Hydroxypropyl Functionality—Strong Affinity Nucleic Acids Binders
2021
New analogs of the commercial asymmetric monomethine cyanine dyes thiazole orange (TO) and thiazole orange homodimer (TOTO) with hydroxypropyl functionality were synthesized and their properties in the presence of different nucleic acids were studied. The novel compounds showed strong, micromolar and submicromolar affinities to all examined DNA ds-polynucleotides and poly rA–poly rU. The compounds studied showed selectivity towards GC-DNA base pairs over AT-DNA, which included both binding affinity and a strong fluorescence response. CD titrations showed aggregation along the polynucleotide with well-defined supramolecular chirality. The single dipyridinium-bridged dimer showed intercalatio…
The Putative Metal Coordination Motif in the Endonuclease Domain of Human Parvovirus B19 NS1 Is Critical for NS1 Induced S Phase Arrest and DNA Damage
2011
The non-structural proteins (NS) of the parvovirus family are highly conserved multi-functional molecules that have been extensively characterized and shown to be integral to viral replication. Along with NTP-dependent helicase activity, these proteins carry within their sequences domains that allow them to bind DNA and act as nucleases in order to resolve the concatameric intermediates developed during viral replication. The parvovirus B19 NS1 protein contains sequence domains highly similar to those previously implicated in the above-described functions of NS proteins from adeno-associated virus (AAV), minute virus of mice (MVM) and other non-human parvoviruses. Previous studies have show…
SISTEMI DI COPERTURE INNOVATIVE NEI CONTESTI ANTICHI
2014
La ricerca è indirizzata al tema delle coperture in vetro a protezione di siti archeologici. Tale scelta è stata influenzata dalle seguenti considerazioni: gli ultimi decenni sono stati caratterizzati da un incremento delle coperture trasparenti protettive; il tema delle strutture di copertura in contesti archeologici e ciò che riguarda la loro progettazione, è stato, e continua ad essere oggi, molto dibattuto tra gli esperti in tutto il mondo; la costante evoluzione tecnologica del vetro continua a incrementare le sue possibilità d’uso in architettura. Tale materiale presenta numerosi vantaggi come l’apparente leggerezza e la trasparenza ma in ambito archeologico pone problematiche che rig…
On site archaeological museums: types of protection
2011
The decision to preserve, in their original context, even the most fragile artefacts, such as floor and wall coverings and to exhibit the delicate stratigraphic or clay manufactures, has favoured the spread of archaeological museums in situ, by interventions aimed at the preservation of archaeological remains. Archaeological museums represent a particular typology capable of relating history through its ruins. Therefore, it is not a museum having typological and functional characteristics that answer to codified museum-graphical and display rules . The arrangement and the public opening of the archaeological sites in recent years have been the object of discussions, debates and interdiscipl…
Proteggere e Valorizzare: una buona pratica per i siti archeologici
2014
Tra i beni culturali quelli archeologici hanno più animato il dibattito culturale sul rapporto tra conservazione e loro messa in valore. La fragilità con cui la materia ci viene consegnata dalla storia, la frammentarietà del reperto, la scarsa disponibilità di risorse finanziarie, anche in relazione alla vastità del patrimonio a noi pervenuto, impongono interventi mirati e talvolta urgenti per la salvaguardia e la conservazione dei beni archeologici. Ma la sola conservazione non può però costituire l’obiettivo ultimo dell’intervento: poiché i beni archeologici rappresentano il nostro lontano passato è anche necessario recuperarne il valore e comunicarne l’identità. La conoscenza storica, st…