Search results for " stem"

In Situ Polyphosphate Nanoparticle Formation in Hybrid Poly(vinyl alcohol)/Karaya Gum Hydrogels: A Porous Scaffold Inducing Infiltration of Mesenchym…

2018

Abstract The preparation and characterization of a porous hybrid cryogel based on the two organic polymers, poly(vinyl alcohol) (PVA) and karaya gum (KG), into which polyphosphate (polyP) nanoparticles have been incorporated, are described. The PVA/KG cryogel is prepared by intermolecular cross‐linking of PVA via freeze‐thawing and Ca2+‐mediated ionic gelation of KG to form stable salt bridges. The incorporation of polyP as amorphous nanoparticles with Ca2+ ions (Ca‐polyP‐NP) is achieved using an in situ approach. The polyP constituent does not significantly affect the viscoelastic properties of the PVA/KG cryogel that are comparable to natural soft tissue. The exposure of the Ca‐polyP‐NP w…

La holografía como recurso didáctico para la enseñanza de contenidos de geometría en primaria

2021

En el presente artículo se analizarán los usos y beneficios que reportaría trabajar con hologramas como material didáctico, como así demuestran los estudios de Ghuloum, Escrivà y Orcos. A través de estas propuestas didácticas se refuerza la idea de que el uso de la técnica de representación en tres dimensiones puede beneficiar al alumnado en la adquisición de los contenidos geométricos presentes en el currículum oficial. El ámbito de la geometría ofrece una amplia gama de contenidos que posibilitan trabajar mediante técnicas de representación gráfica. Cabe destacar, por otra parte, que ahora los estudiantes de educación primaria están socializados en el acceso y uso de imágenes de todo tipo…

Editorial - Connecting the Dots: The Promises of Wharton's Jelly Mesenchymal Stem Cells for Tissue Repair and Regeneration

2011

Mesenchymal stem cells (MSC) constitute a variety of cellular populations which were described first about 35 years ago in the bone marrow (BM) stroma [1]. These years have foreseen an exponential increase of reports pointing out features, stemness, markers, tissue sources and clinical applications of these cells. Cells with MSC features can be isolated from virtually every adult organ in the body, as well from a group of fetus-associated sources (cells derived from the latter tissues are collectively known as perinatal stem cells) [2]. In recent years, the umbilical cord arose as a promising source of mesenchymal stem cells, which can be isolated in relatively high numbers (compared to BM)…

Divided at birth: an in vitro and in situ survey on the expression of immunomodulatory molecules in human Wharton's jelly mesenchymal stem cells and …

2012

HUMAN WHARTON’S JELLY DERIVED MESENCHYMAL STEM CELLS IN PANCREATIC ISLET TRANSPLANTATION

Wharton's jelly mesenchymal stem cells differentiation towards hepatocyte-like cells: functional characterization and expression of immunomodulatory …

2012

Energy Metabolism Analysis of Three Different Mesenchymal Stem Cell Populations of Umbilical Cord Under Normal and Pathologic Conditions

2020

AbstractHuman umbilical cord mesenchymal stem cells (hUC-MSCs) are a pivotal source of therapeutically active cells for regenerative medicine due to their multipotent differentiation potential, immunomodulatory and anti-inflammatory proprieties, as well as logistical collection advantages without ethical concerns. However, it remains poorly understood whether MSCs from different compartments of the human umbilical cord are therapeutically superior than others. In this study, MSCs were isolated from Wharton’s jelly (WJ-MSCs), perivascular region (PV-MSCs) and cord lining (CL-MSCs) of hUC. These cells expressed the mesenchymal markers (CD90, CD73), stemness marker (OCT4), endothelial cell adh…

Umbilical cord revisited: from Wharton’s jelly myofibroblasts to mesenchymal stem cells

2013

The umbilical cord (UC) is an essential part of the placenta, contributing to foetal development by ensuring the blood flow between mother and foetus. The UC is formed within the first weeks of gestation by the enclosure of the vessels (one vein and two arteries) into a bulk of mucous connective tissue, named Wharton’s jelly (WJ) and lined by the umbilical epithelium. Since their first identification, cells populating WJ were described as unusual fibroblasts (or myofibroblasts). Recent literature data further highlighted the functional interconnection between UC and the resident cells. The UC represents a reservoir of progenitor populations which are collectively grouped into MSCs (mesenchy…

Red wine increases the expression of human endothelial nitric oxide synthase

2003

Abstract Objectives The study tested the effect of red wine on endothelial-type nitric oxide synthase (eNOS) expression and eNOS activity in human endothelial cells. Background Endothelial-type nitric oxide (NO) synthase exerts vasoprotective effects. Moderate alcohol consumption has been associated with a reduction of cardiovascular disease, and red wine seems to offer more benefits than any other type of drink. However, the molecular basis of this protective effect is unclear. Methods Human endothelial cells were treated with red wine, and eNOS messenger ribonucleic acid (mRNA) expression was measured by RNase protection assay, eNOS protein expression by Western blotting, and eNOS activit…

Perinatal stem cells revisited: directions and indications at the crossroads between tissue regeneration and repair.

2013

Perinatal stem cells research attracted great interest worldwide in recent years. Foetus-associated tissues contain various populations of stem cells, most of which are comprised within the category of mesenchymal stem cells (MSCs). This special issue collects both reviews and original reports on all the perinatal stem cell types which are currently under investigation. These cells have multiple promising features: differentiative capacity towards mature cell types of all the three germ layers, hypoimmunogenicity in vitro and in vivo, ease of sourcing, ex vivo culture and stor- age. In particular, immune modulation is viewed as a prom- ising feature of many MSCs populations, since these cel…