Search results for " tissues"

Bone Fusion in Normal and Pathological Development is Constrained by the Network Architecture of the Human Skull

2016

The premature fusion of cranial bones, craniosynostosis, affects the correct development of the skull producing morphological malformations in newborns. To assess the susceptibility of each craniofacial articulation to close prematurely, we used a network model of the skull to quantify the link reliability (an index based on stochastic block modeling and Bayesian inference) of each articulation. We show that, of the 93 human skull articulations at birth, the few articulations that are associated with nonsyndromic craniosynostosis conditions have statistically significant lower reliability scores than the others. In a similar way, articulations that close during the normal postnatal developm…

An advanced numerical treatment of EM absorption in human tissue

2020

The numerical computation of local electromagnetic absorption at points within the human tissue is proposed by avoiding the mesh generation in the problem domain. Recently, meshless numerical methods have been introduced as an alter- native computational approach to mesh based methods. This is an important feature to generate competitive procedure able to provide final evaluations for large data amounts in real time. In this paper the smoothed particle hydrodynamics method is considered to compute the electromagnetic absorption. First experiments are performed in two dimension at single frequencies by considering incident TM plane wave on 2D cylinder simulating a simplified model of human t…

Leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5) as a putative human endometrial stem cell marker†

2013

The endometrium is recognized for its remarkable regenerative and remodeling capacity. Every month this hormonally regu- lated organ undergoes cycles of growth (from 0.5-2 to 7 mm), regression and shedding of two-third of the tissue, leading to its monthly renewal that occurs � 400 times in a woman's reproductive lifetime. Several groups have suggested the existence of a human endometrial somatic stem cell (SSC) population located around the spiral arterioles of the basalis. Different groups have isolated, identified and charac- terized putative endometrial SSC populations in human endometrium based on the general features of undifferentiated cells, such as slow cycling detected using the 5…

Correction: DAPIT Over-Expression Modulates Glucose Metabolism and Cell Behaviour in HEK293T Cells

2015

Introduction Diabetes Associated Protein in Insulin-sensitive Tissues (DAPIT) is a subunit of mitochondrial ATP synthase and has also been found to associate with the vacuolar H+-ATPase. Its expression is particularly high in cells with elevated aerobic metabolism and in epithelial cells that actively transport nutrients and ions. Deletion of DAPIT is known to induce loss of mitochondrial ATP synthase but the effects of its over-expression are obscure. Results In order to study the consequences of high expression of DAPIT, we constructed a transgenic cell line that constitutively expressed DAPIT in human embryonal kidney cells, HEK293T. Enhanced DAPIT expression decreased mtDNA content and …

Fast PET Scan Tumor Segmentation Using Superpixels, Principal Component Analysis and K-Means Clustering

2018

Positron Emission Tomography scan images are extensively used in radiotherapy planning, clinical diagnosis, assessment of growth and treatment of a tumor. These all rely on fidelity and speed of detection and delineation algorithm. Despite intensive research, segmentation remained a challenging problem due to the diverse image content, resolution, shape, and noise. This paper presents a fast positron emission tomography tumor segmentation method in which superpixels are extracted first from the input image. Principal component analysis is then applied on the superpixels and also on their average. Distance vector of each superpixel from the average is computed in principal components coordin…

Unsupervised tissue classification of brain MR images for voxel-based morphometry analysis

2016

In this article, a fully unsupervised method for brain tissue segmentation of T1-weighted MRI 3D volumes is proposed. The method uses the Fuzzy C-Means (FCM) clustering algorithm and a Fully Connected Cascade Neural Network (FCCNN) classifier. Traditional manual segmentation methods require neuro-radiological expertise and significant time while semiautomatic methods depend on parameter's setup and trial-and-error methodologies that may lead to high intraoperator/interoperator variability. The proposed method selects the most useful MRI data according to FCM fuzziness values and trains the FCCNN to learn to classify brain’ tissues into White Matter, Gray Matter, and Cerebro-Spinal Fluid in …

Modélisation numérique d’impacts à hautes vitesses par la méthode sans maillage Smoothed Particles Hydrodynamics. Application aux micro-impacts dans …

2021

Investigations on the ballistic behaviours of soft tissue materials are significant research in a medical and pharmaceutical framework. They mainly focus on particles penetrating or traveling into the soft materials. For instance, in the needle-free drug delivery, the micro-scale particles as drug powders are injected into skin with high velocity, and several factors should be considered such as the particle diameters, trajectories, and momentum or velocities of projectile in this process. There have been investigated by some experiments, but there is still no study from the numerical insights.The numerical methods are suitable to study these kinds of processes. Although grid-based methods …

Gene expression of stem cells at different stages of ontological human development.

2013

Abstract Objectives To compare multipotent mesenchymal stem cells (MSCs) obtained from chorionic villi (CV), amniotic fluid (AF) and placenta, with regard to their phenotype and gene expression, in order to understand if MSCs derived from different extra-embryonic tissues, at different stages of human ontological development, present distinct stemness characteristics. Study design MSCs obtained from 30 samples of CV, 30 of AF and 10 placentas (obtained from elective caesarean sections) were compared. MSCs at second confluence cultures were characterized by immunophenotypic analysis with flow cytometry using FACS CANTO II. The expression of the genes Oct-4 (Octamer-binding transcription fact…

[Construction and reaction of the mucosa-associated-lymphatic tissue (MALT) in the middle ear, the Tuba Eustachii and the larynx of the rat].

2003

Extracellular vesicles do not mediate the anti-inflammatory actions of mouse-derived adipose tissue mesenchymal stem cells secretome

2021

Este artículo se encuentra disponible en la siguiente URL: https://www.mdpi.com/1422-0067/22/3/1375 Este artículo pertenece al número especial "The Role of Mesenchymal Stem Cells on Inflammatory and Fibrotic Diseases". Adipose tissue represents an abundant source of mesenchymal stem cells (MSC) for therapeutic purposes. Previous studies have demonstrated the anti-inflammatory potential of adipose tissue-derived MSC (ASC). Extracellular vesicles (EV) present in the conditioned medium (CM) have been shown to mediate the cytoprotective effects of human ASC secretome. Nevertheless, the role of EV in the anti-inflammatory effects of mouse-derived ASC is not known. The current study has investiga…