Search results for " tomato."
showing 10 items of 39 documents
Detection of Southern tomato virus by molecular hybridisation
2017
Overexpression of a cell wall enzyme reduces xyloglucan depolymerization and softening of transgenic tomato fruits.
2010
Xyloglucan xyloglucosyltransferase/endohydrolase (XTHs: EC 2.4.1.207 and/or EC 3.2.1.151) has been proposed to have a dual role integrating newly secreted xyloglucan chains into an existing wall-bound xyloglucan and restructuring existing cell wall material by catalyzing transglucosylation between previously wall bound xyloglucan molecules. In this work we generated transgenic tomatoes with altered levels of an XTH gene. These transgenic fruits showed significant overexpression of the XTH proteins in comparison with the wild type. Specific XET activity was approximately 4.33 fold higher in the transgenic fruits compared with the wild type fruits, although in both cases the activity decrease…
EVOLUZIONE DELL’ATTIVITA’ ANTIOSSIDANTE DI POMODORINO CHERRY DURANTE L’ESSICCAMENTO
2009
Effetti dell’essiccamento sull’attività polifenolossidasica e pattern antiossidante di pomodoro ciliegino(Effects of drying process on polyphenol oxi…
2010
Transcriptome analysis of Pseudomonas mediterranea and P. corrugata plant pathogens during accumulation of medium-chain-length PHAs by glycerol bioco…
2017
Pseudomonas corrugata and P. mediterranea are soil inhabitant bacteria, generally living as endophytes on symptomless plants and bare soil, but also capable of causing plant diseases. They share a similar genome size and a high proteome similarity. P. corrugata produces many biomolecules which play an important role in bacterial cell survival and fitness. Both species produce different medium-chain-length PHAs (mcl-PHAs) from the bioconversion of glycerol to a transparent film in P. mediterranea and a sticky elastomer in P. corrugata. In this work, using RNA-seq we investigated the transcriptional profiles of both bacteria at the early stationary growth phase with glycerol as the carbon sou…
Molecular diversity at the self-incompatibility locus is a salient feature in natural populations of wild tomato (Lycopersicon peruvianum)
1993
A cDNA encoding a stylar protein was cloned from flowers of self-incompatible wild tomato (Lycopersicon peruvianum). The corresponding gene was mapped to the S locus, which is responsible for self-incompatibility. The nucleotide sequence was determined for this allele, and compared to other S-related sequences in the Solanaceae. The S allele was used to probe DNA from 92 plants comprising 10 natural populations of Lycopersicon peruvianum. Hybridization was conducted under moderate and permissive stringencies in order to detect homologous sequences. Few alleles were detected, even under permissive conditions, underscoring the great sequence diversity at this locus. Those alleles that were de…
Proposal of a Genome Editing System for Genetic Resistance to Tomato Spotted Wilt Virus
2014
Viruses provoke considerable economical losses in agriculture. New molecular approaches to develop genetic resistance based on translational genomics and precision genetic modifications are highly expected. The type II Clustered, Regularly Interspaced Palindromic Repeats (CRISPR) system including Cas9 nuclease represent a promising and very powerful tool to specifically modulate the expression and activity of genes involved in biotic stress responses. In this study, we describe an approach to develop a platform system based on CRISPR system for genome editing technology in tomato. Tomato is an excellent plant for this approach considering the high-quality genome sequence, the rapid life cyc…
PRODUCTIVE, COMMERCIAL AND QUALITATIVE CHARACTERIZATION OF SICILIAN LANDRACE TOMATO.
2012
This paper shows the results obtained in 2007 of a study carried out at the Sparacia experimental farm (Cammarata AG, 37°37’N, 13°42’E) aimed at identifying and evaluating 26 populations of “long-life tomato” collected from the Province of Palermo, Agrigento and Trapani. The farm, which belongs to the Sicilian Agricultural Development Agency (E.S.A), is located in a sub-arid area with average rainfall levels of approx. 500 mm and average min/max temperatures of 9 and 21°C. The soil is characterised by level land, deep soil with sandy clay texture and subalkaline soil pH classified as Eutric Vertisols, WRB (FAO-ISRIC-ISSS, 1998). The planting tomato crop was carried out according to traditio…
THE EFFECTS OF IRRIGATION WATER AS A FUNCTION OF THE DISTANCE BETWEEN IRRIGATION DRIPPERS IN A TOMATO FIELD.
2014
The growth and the yield of the tomato plant in southern hot-arid areas of Italy, depending on the satisfaction of water needs. The aim is to evaluate the effect of the distance between drippers on the marketable yield of tomatoes because the installation depend mainly on the number of drippers. Were compared 4 dripper intervals: 15, 30, 60 and 90 cm. The results show that the distance between the drippers must be determined according to the hydraulic characteristics of the soil. In soils with a clay matrix characterized by a low infiltration rate, it seems useful to position the drippers at a distance between 60 and 90 cm.
The Effect of Pesticides on the Tomato Bacterial Speck Disease Pathogen Pseudomonas Syringae pv. Tomato
2020
A significant part of the used pesticides does not reach the target organisms and, while remaining in the agrophytocenosis, influences all living organisms in it. Having a toxic and often mutagenic effect, pesticides induce morphological and physiological changes in the cells of microorganisms and are the cause of phenotypic heterogeneity of their populations. However, the effect of pesticides on phytopathogenic bacteria as non-target microorganisms remains out of the field of view for most researchers. However, the use of pesticides can lead to expansion of the diversity of existing phytopathogens and, as a consequence, complications of identification of the pathogens, loss of resistance b…