Search results for " transfection"

showing 2 items of 22 documents

Magnetofection of acoustically active „magnetic microspheres“ (Magnetobubbles) — Induction of angiogenesis via non-viral transfection of VEGF

2009

Adenoviral transduction of the VEGF gene in an oversized skin flap increases flap survival and perfusion. In this study we investigated the potential of magnetofection of magnetic lipospheres containing VEGF165-cDNA on survival and perfusion of ischemic skin flaps and evaluated the method with respect to the significance of applied magnetic field and ultrasound.

integumentary systembiologybusiness.industryChemistryAngiogenesisVEGF receptorsUltrasoundSkin flapequipment and suppliesNon viral transfectioneye diseasesMicrospherebiology.proteinMagnetofectionCancer researchbusinesshuman activitiesPerfusion
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Systematic Study of a Library of PDMAEMA-Based, Superparamagnetic Nano-Stars for the Transfection of CHO-K1 Cells.

2017

The introduction of the DNA into mammalian cells remains a challenge in gene delivery, particularly in vivo. Viral vectors are unmatched in their efficiency for gene delivery, but may trigger immune responses and cause severe side-reactions. Non-viral vectors are much less efficient. Recently, our group has suggested that a star-shaped structure improves and even transforms the gene delivery capability of synthetic polycations. In this contribution, this effect was systematically studied using a library of highly homogeneous, paramagnetic nano-star polycations with varied arm lengths and grafting densities. Gene delivery was conducted in CHO-K1 cells, using a plasmid encoding a green fluore…

magnetic nanoparticlesPDMAEMAPolymers and PlasticsEGFP02 engineering and technologyATRPPDEGMAGene delivery010402 general chemistry01 natural sciencesArticleViral vectorGreen fluorescent proteinpolycationchemistry.chemical_compoundPlasmidIn vivogene deliveryChemistryChinese hamster ovary cellcellular uptakeCHO cellsGeneral ChemistryTransfection021001 nanoscience & nanotechnologyMolecular biology0104 chemical sciencestransfectionBiophysics0210 nano-technologyEthylene glycolATRP; cellular uptake; CHO cells; EGFP; gene delivery; magnetic nanoparticles; PDMAEMA; PDEGMA; polycation; transfectionPolymers
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