Search results for " trip"
showing 10 items of 535 documents
Multiple roles for ISWI in transcription, chromosome organization and DNA replication.
2003
ISWI functions as the ATPase subunit of multiple chromatin-remodeling complexes. These complexes use the energy of ATP hydrolysis to slide nucleosomes and increase chromatin fluidity, thereby modulating the access of transcription factors and other regulatory proteins to DNA. Here we discuss recent progress toward understanding the biological functions of ISWI, with an emphasis on its roles in transcription, chromosome organization and DNA replication.
Manipulating mtDNA in vivo reprograms metabolism via novel response mechanisms.
2019
Mitochondria have been increasingly recognized as a central regulatory nexus for multiple metabolic pathways, in addition to ATP production via oxidative phosphorylation (OXPHOS). Here we show that inducing mitochondrial DNA (mtDNA) stress in Drosophila using a mitochondrially-targeted Type I restriction endonuclease (mtEcoBI) results in unexpected metabolic reprogramming in adult flies, distinct from effects on OXPHOS. Carbohydrate utilization was repressed, with catabolism shifted towards lipid oxidation, accompanied by elevated serine synthesis. Cleavage and translocation, the two modes of mtEcoBI action, repressed carbohydrate rmetabolism via two different mechanisms. DNA cleavage activ…
Applying Visual Analysis Methods for mapping Emotions Felt along Daily Trips
2013
International audience; Visual analytics are tools and methods allowing the visual analysis of large sets of movement data. We here use these methods to analyze intra-urban daily trips of individuals as well as the emotion felt by the individuals along their trip. The interdisciplinary research project ECDESUP (Evaluation, Choice and Decision in the use of Urban and Peri-Urban Spaces, www.ecdesup.org/) allowed us to obtain the data via a survey done in the town of Besançon (Eastern France). The first objective was to determine if some places in the city gather a specific type of emotions (either positive or negative) with respect to the day of the week and/or the time of the day. The second…
Changes in the proton potential and the cellular energetics of Escherichia coli during growth by aerobic and anaerobic respiration or by fermentation.
1998
The energetic parameters of Escherichia coli were analyzed for the aerobic/anaerobic transition. The electrochemical proton potential (delta p) across the cytoplasmic membrane was determined in the steady state of respiration with O2, nitrate, fumarate, dimethylsulfoxide (Me2SO), and for fermentation. With O2, a proton potential of -160 mV was obtained. For anaerobic respiration with nitrate, fumarate or Me2SO, delta p decreased only slightly by about 20 mV in contrast to earlier assumptions, whereas delta p dropped by approximately 40 mV during fermentation. Under all conditions, the membrane potential (delta psi) contributed the major portion to delta p. The cellular ATP levels were highe…
From the covalent linkage of drugs to novel inhibitors of ribonucleotide reductase: synthesis and biological evaluation of valproic esters of 3'-C-me…
2014
We synthesized a series of serum-stable covalently linked drugs derived from 3'-C-methyladenosine (3'-Me-Ado) and valproic acid (VPA), which are ribonucleotide reductase (RR) and histone deacetylase (HDAC) inhibitors, respectively. While the combination of free VPA and 3'-Me-Ado resulted in a clear synergistic apoptotic effect, the conjugates had lost their HDAC inhibitory effect as well as the corresponding apoptotic activity. Two of the analogs, 2',5'-bis-O-valproyl-3'-C-methyladenosine (A160) and 5'-O-valproyl-3'-C-methyladenosine (A167), showed promising cytotoxic activities against human hematological and solid cancer cell lines. A167 was less potent than A160 but had interesting featu…
Insights into how nucleotide supplements enhance the peroxidase-mimicking DNAzyme activity of the G-quadruplex/hemin system
2012
Since the initial discovery of the catalytic capability of short DNA fragments, this peculiar enzyme-like property (termed DNAzyme) has continued to garner much interest in the scientific community because of the virtually unlimited applications in developing new molecular devices. Alongside the exponential rise in the number of DNAzyme applications in the last past years, the search for convenient ways to improve its overall efficiency has only started to emerge. Credence has been lent to this strategy by the recent demonstration that the quadruplex-based DNAzyme proficiency can be enhanced by ATP supplements. Herein, we have made a further leap along this path, trying first of all to deci…
Un diritto di agire in modo penalmente illecito?
2005
Analisi critica della idea che possa esistere un diritto di agire in modo penalmente illecito, e che tale diritto possa, in particolare, localizzarsi nella categoria delle cosiddette cause di giustificazione.
On the additivity of block designs
2016
We show that symmetric block designs $${\mathcal {D}}=({\mathcal {P}},{\mathcal {B}})$$D=(P,B) can be embedded in a suitable commutative group $${\mathfrak {G}}_{\mathcal {D}}$$GD in such a way that the sum of the elements in each block is zero, whereas the only Steiner triple systems with this property are the point-line designs of $${\mathrm {PG}}(d,2)$$PG(d,2) and $${\mathrm {AG}}(d,3)$$AG(d,3). In both cases, the blocks can be characterized as the only k-subsets of $$\mathcal {P}$$P whose elements sum to zero. It follows that the group of automorphisms of any such design $$\mathcal {D}$$D is the group of automorphisms of $${\mathfrak {G}}_\mathcal {D}$$GD that leave $$\mathcal {P}$$P in…
The Go2School project for promoting cycling to school: A case study in Palermo
2021
Abstract The identification of transport policy measures able to reduce the use of private cars for home-to-school travel is very relevant to reduce congestion during peak hours and to ensure that the areas around schools have livable environments. An action that policymakers could apply is promoting cycling to school through the introduction of bikesharing programs and creating safe routes to school through the construction of new cycle infrastructure. The aim of the paper has been, therefore, to assess if these policies could lead the high-school students to cycle to school, considering the city of Palermo as a case study. The goal is reached through the calibration of a modal choice mode…
Différences de genre et formes de dépendances des conjoints biactifs dans l'accompagnement des enfants
2011
International audience; L'approche des différences de genre dans la mobilité a été renouvelée à partir des années 2000 par l'attention portée aux formes d'interdépendance entre conjoints pour leurs déplacements quotidiens. Dans ce travail mobilisant l'enquête ménages-déplacements d'Île-de-France, nous en avons relevé deux types à partir de l'analyse de l'accompagnement des enfants des conjoints de couples de biactifs. D'une part, il y a un effet d'entraînement. Lorsqu'un conjoint fait de l'accompagnement, l'autre a une probabilité plus forte d'en faire également. D'autre part, les conjoints se distribuent entre eux l'accompagnement avant travail et après travail. Toutefois, l'interdépendanc…