Search results for " trip"
showing 10 items of 535 documents
Agent-based model for the rescheduling of Individual and collective daily activities under uncertainties
2020
Daily activity schedule are popular for people duringdaily life. While, when executing the schedule on the real road network, there are always some disruptions disturbing the planned schedule. To deal with this problem, daily activity rescheduling is necessary. This thesis regards the disruptions from the activity schedule execution environment as unexpected events (uncertainties). It establishes agent-based models to simulate the activity rescheduling decision process from the aspects of individual activity rescheduling and joint trip renegotiating.For the individual activity rescheduling, the model in this thesis wants to explore the relationship between a pair of episodes (two connected …
Resealing of large transmembrane pores produced by streptolysin O in nucleated cells is accompanied by NF‐κB activation and downstream events
2001
Streptolysin O (SLO), archetype of a cholesterol-binding bacterial cytolysin, forms large pores in the plasma membrane of mammalian cells. We have recently reported that when a limited number of pores are generated in a cell, they can be sealed in a Ca++-dependent process. Here, we show that resealing is followed by the release of IL-6 and IL-8 from keratinocytes and from endothelial cells, both relevant targets for SLO attack. Production of cytokines by these cells was preceded by activation of transcription factor nuclear factor kappaB, which thus emerges as a common denominator of stress responses to various pore-forming agents, including alpha-toxin of Staphylococcus aureus and compleme…
Melittin Modulates Keratinocyte Function through P2 Receptor-dependent ADAM Activation
2012
Melittin, the major component of the bee venom, is an amphipathic, cationic peptide with a wide spectrum of biological properties that is being considered as an anti-inflammatory and anti-cancer agent. It modulates multiple cellular functions but the underlying mechanisms are not clearly understood. Here, we report that melittin activates disintegrin-like metalloproteases (ADAMs) and that downstream events likely contribute to the biological effects evoked by the peptide. Melittin stimulated the proteolysis of ADAM10 and ADAM17 substrates in human neutrophil granulocytes, endothelial cells and murine fibroblasts. In human HaCaT keratinocytes, melittin induced shedding of the adhesion molecu…
Differential role of p38 mitogen activated protein kinase for cellular recovery from attack by pore-forming S. aureus alpha-toxin or streptolysin O.
2006
Following the observation that cells are able to recover from membrane lesions incurred by Staphylococcus aureus alpha-toxin and streptolysin O (SLO), we investigated the role of p38 in this process. p38 phosphorylation occurred in response to attack by both toxins, commencing within minutes after toxin treatment and waning after several hours. While SLO reportedly activates p38 via ASK1 and ROS, we show that this pathway does not play a major role for p38 induction in alpha-toxin-treated cells. Strikingly divergent effects of p38 blockade were noted depending on the toxin employed. In the case of alpha-toxin, inhibition of p38 within the time frame of its activation led to disruption of th…
Leggere il viaggio: la prospettiva dei geografi
2020
Il saggio intende approfondire i temi del viaggio e del pellegrinaggio attraverso l'analisi della scrittura letteraria e dei multiformi significati che le due esperienze, quella del viaggio e del pellegrinaggio, rappresentano. Il viaggio è anche l’espressione dell’osservazione diretta, uno dei più importanti metodi dell’indagine geografica che permette fin da piccoli di acquisire la capacità di studiare il paesaggio, l’ambiente e il rapporto individuo-natura. Esso fornisce input di riflessione, stimola domande, pone questioni anche di rilievo globale. Oggetto del viaggio, inteso come esperienza di conoscenza, è spesso il paesaggio, per i Geografi un concetto tanto complesso quanto affascina…
Nuove ricerche sul tempio della Magna Mater a Leptis Mgna
2016
L'articolo presenta la ricerca sui resti dell tempio della Magna Mater nel Foro Vecchio di Leptis Magna. A partire dal 2006 l'edificio è stato accuratamente rilevato ed è stato avviato lo studio sistematico. Il tempio venne edificato in età Flavia, come testimonia l'iscrizione IRT 300, che tramanda anche il nome del finanziatore: Iddibal figlio di Basillec, nipote di Annobal, pronipote di Asmun.
Human Hsp60 with Its Mitochondrial Import Signal Occurs in Solution as Heptamers and Tetradecamers Remarkably Stable over a Wide Range of Concentrati…
2014
It has been established that Hsp60 can accumulate in the cytosol in various pathological conditions, including cancer and chronic inflammatory diseases. Part or all of the cytosolic Hsp60 could be naive, namely, bear the mitochondrial import signal (MIS), but neither the structure nor the in solution oligomeric organization of this cytosolic molecule has still been elucidated. Here we present a detailed study of the structure and self-organization of naive cytosolic Hsp60 in solution. Results were obtained by different biophysical methods (light and X ray scattering, single molecule spectroscopy and hydrodynamics) that all together allowed us to assay a wide range of concentrations of Hsp60…
The minimal model of Hahn for the Calvin cycle.
2018
There are many models of the Calvin cycle of photosynthesis in the literature. When investigating the dynamics of these models one strategy is to look at the simplest possible models in order to get the most detailed insights. We investigate a minimal model of the Calvin cycle introduced by Hahn while he was pursuing this strategy. In a variant of the model not including photorespiration it is shown that there exists exactly one positive steady state and that this steady state is unstable. For generic initial data either all concentrations tend to infinity at lates times or all concentrations tend to zero at late times. In a variant including photorespiration it is shown that for suitable v…
Photoaffinity Labeling and Photoaffinity Cross-Linking of Phosphofructokinase-1 from Saccharomyces cerevisiae by 8-Azidoadeninenucleotides
2001
Phosphofructokinase-1 from Saccharomyces cerevisiae is composed of four alpha- and four beta-subunits, each of them carrying catalytic and regulatory bindings sites for MgATP. In this paper, various photoaffinity labels, such as 8-azidoadenosine 5'-triphosphate, 8-azido-1,N6-ethenoadenosine 5'-triphosphate, and 8-N3-3'(2')-O-biotinyl-8-azidoadenosine 5'-triphosphate have been used to study their interaction with the enzyme in the dark and during irradiation. All nucleotidetriphosphates function as phosphate donor forming fructose 1,6-bisphosphate from fructose 6-phosphate. However, the kinetic analysis revealed distinctly differences between them. Photolabeling causes a decrease in enzyme a…
A theoretical insight into the photophysics of psoralen
2006
Psoralen photophysics has been studied on quantum chemistry grounds using the multiconfigurational second-order perturbation method CASPT2. Absorption and emission spectra of the system have been rationalized by computing the energies and properties of the low-lying singlet and triplet excited states. The S1 ππ* state has been determined to be responsible of the lowest absorption and fluorescence bands and to initially carry the population in the photophysical processes related to the phototherapeutic properties of psoralen derivatives. The low-lying T1 ππ* state is, on the other hand, protagonist of the phosphorescence, and its prevalent role in the reactivity of psoralen is suggested to b…