Search results for " trip"

An isoperimetric type problem for primitive Pythagorean hodograph curves

2012

An isoperimetric type problem for primitive Pythagorean hodograph curves is studied. We show how to compute, for each possible degree, the Pythagorean hodograph curve of a given perimeter enclosing the greatest area. We also discuss the existence and construction of smooth solutions, obtaining a relationship with an interesting sequence of Appell polynomials.

Differential changes in purine nucleotides after Doxorubicin treatment of human cancer cells in vitro

2002

The present investigation was performed to elucidate the role of purine nucleotides as potential indicators of chemosensitivity of malignant tumors. Drug-sensitive (s) and -resistant (r) tumor cell lines grown as monolayers (s: T47D, MCF-7 wild-type; r: NCI/ADR-RES, MCF-7/MDR) or as multicellular spheroids (T47D; NCI/ADR-RES) were exposed to 0.1, 1.0, and 10.0 microM Doxorubicin for up to 24 h. Purine nucleotides were assayed using HPLC and with some selected spheroids using imaging bioluminescence. The data show that in the time frame of the experiments reproducible and statistically significant changes in the nucleotides only occur at the highest drug concentration investigated. Under the…

The GRP94 Inhibitor PU-WS13 Decreases M2-like Macrophages in Murine TNBC Tumors: A Pharmaco-Imaging Study with 99mTc-Tilmanocept SPECT

2021

Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancers and is not eligible for hormone and anti-HER2 therapies. Identifying therapeutic targets and associated biomarkers in TNBC is a clinical challenge to improve patients’ outcome and management. High infiltration of CD206+ M2-like macrophages in the tumor microenvironment (TME) indicates poor prognosis and survival in TNBC patients. As we previously showed that membrane expression of GRP94, an endoplasmic reticulum chaperone, was associated with the anti-inflammatory profile of human PBMC-derived M2 macrophages, we hypothesized that intra-tumoral CD206+ M2 macrophages expressing GRP94 may represent innovative…

Clathrin-independent entry of baculovirus triggers uptake of E. coli in non-phagocytic human cells

2008

The prototype baculovirus, Autographa californica multiple nucleopolyhedrovirus, an insect pathogen, holds great potential as a gene therapy vector. To develop transductional targeting and gene delivery by baculovirus, we focused on characterizing the nature and regulation of its uptake in human cancer cells. Baculovirus entered the cells along fluid-phase markers from the raft areas into smooth-surfaced vesicles devoid of clathrin. Notably, regulators associated with macropinocytosis, namely EIPA, Pak1, Rab34, and Rac1, had no significant effect on viral transduction, and the virus did not induce fluid-phase uptake. The internalization and nuclear uptake was, however, affected by mutants o…

GALACTOSE-FUNCTIONALISED POLYMERIC MICELLES AS HEPATOCYTE-TARGETED CARRIERS

2012

Arabinose nucleoside triphosphates are no inhibitors for DNA-dependent RNA polymerases.

1976

1-Beta-D-arabinofuranosylcytosine-5' -triphosphate and 9-beta-D-arabinofuranosyladenosine-5' -triphosphate were found to have no inhibitory potency for both mammalian DNA-dependent RNA polymerase II and E. coli DNA-dependent RNA polymerase.

Contextuality Analysis of the Double Slit Experiment (With a Glimpse Into Three Slits)

2018

The Contextuality-by-Default theory is illustrated on contextuality analysis of the idealized double-slit experiment. The experiment is described by a system of contextually labeled binary random variables each of which answers the question: has the particle hit the detector, having passed through a given slit (left or right) in a given state (open or closed)? This system of random variables is a cyclic system of rank 4, formally the same as the system describing the EPR/Bell paradigm with signaling. Unlike the latter, however, the system describing the double-slit experiment is always noncontextual, i.e., the context-dependence in it is entirely explainable in terms of direct influences of…

α1-Adrenoceptors in the rat cerebral cortex: New insights into the characterization of α1L- and α1D-adrenoceptors

2010

36 p., figuras y tablas, bibliografía

Structural dynamics in F1ATPase during the first reaction cycle of ATP hydrolysis

1991

Abstract The velocity of ATP hydrolysis, catalyzed by purified F 1 ATPase from Micrococcus luteus , was decelerated on decreasing the temperature. At 13′C one reaction cycle is completed after 20 s. Hydrolysis was triggered upon rapid mixing of the enzyme with ATP. During the first reaction cycle, succeeding structural alterations of the F 1 ATPase were traced by time resolved X-ray scattering. The scattering spectra obtained from consecutive intervals of 1 s, revealed the F 1 ATPase to pass a conformational state exhibiting an expanded (6%) molecular shape. The expanded state was observed between 45% and 65% of the time required to complete the reaction cycle. This pointx out a conformatio…

Saint Benedict staring at us: Clients as the third party in Modern Art. A theoretical model and a case study in Renaissance Venice

2014

Il presente saggio intende approfondire il ruolo della committenza quale terza parte nel sistema dell’arte in Età Moderna. Al fine di identificare un possibile rapporto di terzietà nell’arte, l’approccio di ricerca si fonda sulla sociologia dell’arte e si concentra sul funzionamento economico della produzione artistica, pur sfruttando infine la tradizione dei casi di studio di matrice iconologica per verificare la correttezza del modello proposto. L’esempio sotto osservazione fa riferimento al Rinascimento Veneziano trattandosi del Trittico dei Frari di Giovanni Bellini, realizzato alla fine del XV secolo e concernente la disputa sull’Immacolata Concezione della Madonna. This paper aims at …