Search results for " vivo"
showing 10 items of 1661 documents
DA-Phen, a new dopamine aminoacid conjugate: in vivo testing and molecular modeling as dopaminergic modulator
2014
Live imaging of developmental processes in a living meristem of Davidia involucrata (Nyssaceae).
2014
Morphogenesis in plants is usually reconstructed by scanning electron microscopy and histology of meristematic structures. These techniques are destructive and require many samples to obtain a consecutive series of states. Unfortunately, using this methodology the absolute timing of growth and complete relative initiation of organs remain obscure. To overcome this limitation, an in vivo observational method based on Epi-Illumination Light Microscopy (ELM) was developed and tested with a male inflorescence meristem (floral unit) of the handkerchief tree Davidia involucrata Baill. (Nyssaceae). We asked whether the most basal flowers of this floral unit arise in a basipetal sequence or, altern…
Singēna ortotopiska prostatas audzēja modeļa izveide imūnkompetentās pelēs vēža šūnu producēto ekstracelulāro vezikulu pato-fizioloģiskās lomas pētīj…
2018
Prostatas vēzis vēl joprojām ir nozīmīga veselības problēma visā pasaulē un ir parādīts ka ekstracelulārās vezikulas (EV) ir nozīmīgs faktors ļaundabīga audzēja attīstībā. Šī darba gaitā tika izveidots singēns ortotopisks prostatas audzēja modelis C57BL/6 tipa pelēs vēža šūnu producēto EV patofizioloģiskās lomas pētījumiem in vivo. Iegūtie dati liecina par mikrometastāžu veidošanos aknās un ar kuņģi asociētajās audu struktūrās pelēs ar ortotopisku audzēju, kurā veiktas intratumorālas metastātisko TRAMP-C2 šūnu hipoksijā producēto EV injekcijas, bet ne citu grupu dzīvniekiem, kas norāda uz modeļa potenciālu tālākiem EV pētījumiem. Tomēr nepieciešams ieviest izmaiņas, kas dotu iespēju turpmāk…
Tolerance and M2 (alternative) macrophage polarization are related processes orchestrated by p50 nuclear factor {kappa}B.
2009
Cells of the monocyte-macrophage lineage play a central role in the orchestration and resolution of inflammation. Plasticity is a hallmark of mononuclear phagocytes, and in response to environmental signals these cells undergo different forms of polarized activation, the extremes of which are called classic or M1 and alternative or M2. NF-kappaB is a key regulator of inflammation and resolution, and its activation is subject to multiple levels of regulation, including inhibitory, which finely tune macrophage functions. Here we identify the p50 subunit of NF-kappaB as a key regulator of M2-driven inflammatory reactions in vitro and in vivo. p50 NF-kappaB inhibits NF-kappaB-driven, M1-polariz…
Dietary water seems to improve skin hydration without influencing hemodynamics
2013
The various studies on the importance of water in the hydration of human skin have suggested that an increased dietary intake might positively impact the physiology of normal skin. We evaluated the consequences of increasing water intake through drinking in skin hydration in vivo. Eating habits were maintained, evaluated by a Food Frequency Questionnaire and based on total water consumption. In two groups with different total water consumption, we evaluated the impact of 2L/day water after 30 days,. This increase produced no hemodynamic changes or weight changes but an improvement in the epidermal hydration was suggested. The magnitude of the impact on skin hydration was higher in the group…
Pienskābo baktēriju izdalīšana un identifikācija no Latvijā ražotiem svaigiem un tradicionāli sālītiem gurķiem ar mērķi inhibēt svaigo dārzeņu patogē…
2019
Šī darba mērķis bija izstrādāt svaigu dārzeņu apstrādes metodi, lai aizsargātu tos pret mikroorganismiem, kuri izraisa augu slimības un ir saistīti ar svaigu dārzeņu kvalitātes pasliktināšanos. Mērķa sasniegšanai izmantoja mikroorganismu spējas inhibēt citu mikroorganismu augšanu (antagonismu). Ir pierādīts, ka pienskābes baktērijām piemīt antagonistiskās spējas. Tāpēc par izpētes objektu bija izvēlētas pienskābes baktērijas, kuras bija izolētas no vietējo ražotāju svaigiem un sālītiem gurķiem. No svaigiem un sālītiem gurķiem tika izdalīti 133 pienskābo baktēriju izolāti, kuri pieder sekojošām identificētām sugām: Leuconostoc citreum Leuconostoc mesentoides, Lactobacillus buchneri, Lactobac…
Viewpoint: On the hysteresis in the human Achilles tendon
2013
Synthesis and preliminary in vivo evaluation of well-dispersed biomimetic nanocrystalline apatites labeled with positron emission tomographic imaging…
2015
In recent years, biomimetic synthetic apatite nanoparticles (AP-NPs), having chemical similarity with the mineral phase of bone, have attracted a great interest in nanomedicine as potential drug carriers. To evaluate the therapeutic perspectives of AP-NPs through the mechanisms of action and organs they interact with, the noninvasive monitoring of their in vivo behavior is of paramount importance. To this aim, here the feasibility to radiolabel AP-NPs ("naked" and surface-modified with citrate to reduce their aggregation) with two positron emission tomographic (PET) imaging agents ([F-18]NaF and Ga-68-NO(2)AP(BP)) was investigated. [F-18]NaF was used for the direct incorporation of the radi…
Capsaicin desensitization in vivo is inhibited by ruthenium red.
1990
The effect of systemic administration of Ruthenium Red on the excitatory and desensitizing effect of capsaicin was investigated in rats. Ruthenium Red was injected s.c. 30 min before capsaicin was administered. The excitatory effect of capsaicin on corneal, perivascular and visceral afferents was not influenced by treatment with Ruthenium Red. However, determination of the neuropeptide content and evoked neuropeptide release in peripheral organs and dorsal spinal cord 48 h after treatment showed that Ruthenium Red attenuated the 'desensitizing' effect of capsaicin at peripheral, but not at central, endings of primary afferents. On the other hand, a capsaicin-elicited autonomic reflex mediat…
Increased carrier peptide stability through ph adjustment improves insulin and pth(1-34) delivery in vitro and in vivo rather than by enforced carrie…
2020
Oral delivery of therapeutic peptides is hampered by their large molecular size and labile nature, thus limiting their permeation across the intestinal epithelium. Promising approaches to overcome the latter include co-administration with carrier peptides. In this study, the cell-penetrating peptide penetratin was employed to investigate effects of co-administration with insulin and the pharmacologically active part of parathyroid hormone (PTH(1-34)) at pH 5, 6.5, and 7.4 with respect to complexation, enzymatic stability, and transepithelial permeation of the therapeutic peptide in vitro and in vivo. Complex formation between insulin or PTH(1-34) and penetratin was pH-dependent. Micron-size…