Search results for " vivo"
showing 10 items of 1661 documents
PET Imaging of the Impact of Extracellular pH and MAP Kinases on the p-Glycoprotein (Pgp) Activity
2012
The functional activity of p-glycoprotein (Pgp) can be increased in vitro by an extracellular acidosis via activation of MAP kinases (p38, ERK1/2). In order to study these effects in vivo a new (68)Ga-labeled PET tracer was developed which serves as a substrate of the Pgp and therefore indirectly mirrors the Pgp activity. For in vivo studies, experimental tumors were imaged under acidic conditions (inspiratory hypoxia, injection of lactic acid) and during inhibition of MAP kinases in a μ-PET system. In vitro, [(68)Ga]MFL6.MZ showed an accumulation within the cells of about 20% which was increased to 30% by Pgp inhibition. In solid tumors a marked tracer uptake was observed showing spatial h…
Juego transformado en subcultura. Un análisis del Live-Actio Role-Playing (LARP) como medio de ocio y arte
2015
El LARP es una actividad que procede por una parte de los juegos de rol clásicos, y por otra, de las artes escénicas. Se basa en la interpretación de un personaje de manera improvisada en contacto con otros personajes, es por eso que suele decirse que el LARP es teatro alternativo o interactivo. Además, no existe un público a la manera habitual, sino que el público participa en el propio evento, y todos y cada uno de los participantes son público al mismo tiempo. Los personajes forman parte de una historia que va formándose entre todos los participantes, y que nunca puede predecirse como acabará, ya que es decisión de los propios participantes. Debemos tener en cuenta que alrededor de esta …
Explorando las desigualdades sociales y formas de contrarrestarlas
2019
Production of INCASI Project H2020-MSCA-RISE-2015 GA 691004 La propuesta de este artículo es presentar una experiencia actual de innovación docente en la que se vincula la enseñanza de la Sociología con la investigación desarrollada en el proyecto europeo INCASI: International Network for Comparative Analysis of Social Inequalities. La experiencia docente se aplica en la asignatura de Sociología, con alumnado de primer curso, del grado de Gestión y Administración Pública, de la Facultad de Derecho, de la Universidad de Barcelona. El eje central del trabajo desarrollado por el alumnado es realizar una investigación sociológica exploratoria sobre una desigualdad social y observar e idear polí…
Comparison of a near-infrared reflectance spectroscopy system and skin conductance measurements for in vivo estimation of skin hydration: a clinical …
2017
Diffuse reflectance spectroscopy system was developed for estimation of skin hydration in the near-infrared spectral range of 900-1700 nm. Experimental setup consisted of a near-infrared spectrometer, Y-type fiber optics probe with 1 detection and 6 illumination fibers, halogen-tungsten light source and a PC. By analyzing diffuse reflectance spectrum, a parameter representing skin hydration by performing baseline correction and calculating the area under the 1450 nm water absorption maximum is proposed. A clinical study was performed acquiring data of skin hydration of 39 patients' forearm skin. Results of the developed system are compared to results obtained by a commercial device based on…
The prognostic and predictive value of IKZF1 and IKZF3 expression in T-cells in patients with multiple myeloma
2018
Purpose: While recent studies described the role of IKZF1/3 proteins in multiple myeloma (MM) cells, few have highlighted the significance of IKZF1/3 expression in T-cells. In this study we examine the prognostic and predictive value of IKZF1/3 expression in T-cells in patients with MM stage III. Experimental design: We analysed the IKZF1/3 expression levels in T-cells from 45 MM stage I (MMI) and 50 newly diagnosed MM stage III (MMIII) patients, according to Durie-Salmon staging system, by flow cytometry to examine their prognostic and predictive value. We also combined in vivo observations with in vitro assays to determine the effect of IKZF1/3 expression on the T-cell immunophenotype and…
Macrophage: SHIP of Immunity
2014
Immunology. Why does it exist? Two words. Cure disease. People get diseases. “Test tubes” do not. People fund immunologists for solutions to their health problems. But, immunologists often study leukocytes in test tubes – the laboratory – away from diseases. Why? Because much can be learned from analyzing cellular biochemistry and behaviors in vitro that cannot be ascertained when leukocytes are in animals. At the same time, isolated leukocyte reactions often do not reflect how the immune system operates as a unit. So, it is critical to verify in vitro observations in vivo. Among leukocytes, macrophages are the central initiating and directing element in immune systems, and serve this role …
Vγ9Vδ2 T cells as a promising innovative tool for immunotherapy of hematologic malignancies
2011
The potent anti-tumor activities of γδ T cells, their ability to produce pro-inflammatory cytokines, and their strong cytolytic activity have prompted the development of protocols in which γδ agonists or ex vivo-expanded γδ cells are administered to tumor patients. γδ T cells can be selectively activated by either synthetic phosphoantigens or by drugs that enhance their accumulation into stressed cells as aminobisphosphonates, thus offering new avenues for the development of γδ T cell-based immunotherapies. The recent development of small drugs selectively activating Vγ9Vδ2 T lymphocytes, which upregulate the endogenous phosphoantigens, has enabled the investigators to design the experiment…
Bone targeting compounds for radiotherapy and imaging: *Me(III)-DOTA conjugates of bisphosphonic acid, pamidronic acid and zoledronic acid
2016
Background Bisphosphonates have a high adsorption on calcified tissues and are commonly used in the treatment of bone disorder diseases. Conjugates of bisphosphonates with macrocyclic chelators open new possibilities in bone targeted radionuclide imaging and therapy. Subsequent to positron emission tomography (PET) examinations utilizing 68Ga-labelled analogues, endoradiotheraphy with 177Lu-labelled macrocyclic bisphosphonates may have a great potential in the treatment of painful skeletal metastases. Methods Based on the established pharmaceuticals pamidronate and zoledronate two new DOTA-α-OH-bisphosphonates, DOTAPAM and DOTAZOL(MM1.MZ) were successfully synthesized. The ligands were labe…
Influence of Nanoparticle Exposure on Nervous System Development in Zebrafish Studied by Means of Light Sheet Fluorescence Microscopy
2016
Zebrafish has a remarkable similarity in the molecular signaling processes, cellular structure, anatomy and physiology to other higher order vertebrates, making it an excellent vertebrate model organism (1). Recently, zebrafish has been used for neurotoxicity screening of numerous nanomaterials with a focus on the developmental effects due to the possibility of in vivo visualization of specific neurons and axon tracts by injecting dyes in live animals as well in fixed ones (2). Here we propose Light Sheet Fluorescence Microscopy (LSFM) (3) (4) to perform neurotoxicity studies, in order to study the nervous system architecture and to image 3D structures in the brain of live larvae during the…
Circulating Tumor Cells (CTCs): A Unique Model of Cancer Metastases and Non-invasive Biomarkers of Therapeutic Response
2021
Late-stage cancer metastasis remains incurable in the clinic and is the major cause death in patients. Circulating tumor cells (CTCs) are thought to be metastatic precursors shed from the primary tumor or metastatic deposits and circulate in the blood. The molecular network regulating CTC survival, extravasation, and colonization in distant metastatic sites is poorly defined, largely due to challenges in isolating rare CTCs. Recent advances in CTC isolation and ex vivo culture techniques facilitates single-cell omics and the development of related animal models to study CTC-mediated metastatic progression. With these powerful tools, CTCs can potentially be used as non-invasive biomarkers pr…