Search results for "β"

No ķirurģiskā materiāla izdalīto Klebsiella spp. baktēriju β-laktamāžu producēšanas noteikšana

2021

Klebsiella pneumoniae un Klebsiella oxytoca ir Gram-negatīvas nosacīti patogēnas baktērijas, kas izraisa urīnceļu, plaušu u.c. orgānu infekcijas. Klebsiella spp. raksturo plaša rezistence pret β-laktāmu antibakteriālajiem līdzekļiem, kur klīniski nozīmīgāka ir paplašināta darbības spektra β-laktamāze (ESBL). Darbā apkopota literatūra par Klebsiella spp. morfoloģiju, infekcijām, antibakteriālu terapiju un tās rezistences mehānismiem. Praktiskajā daļā tika iegūti Klebsiella spp. antibakteriālas jutības dati, pamatojoties uz disku difūzijas testiem (DDT), E-testiem, un arī lietojot molekulārbioloģiskās izmeklēšanas metodes: DNA STRIP® tehnoloģiju. Pētījums tika veikts Rīgas Traumatoloģijas un …

Primary culture of avian embryonic heart forming region cells to study the regulation of vertebrate early heart morphogenesis by vitamin A

2014

Background: Important knowledge about the role of vitamin A in vertebrate heart development has been obtained using the vitamin A-deficient avian in ovo model which enables the in vivo examination of very early stages of vertebrate heart morphogenesis. These studies have revealed the critical role of the vitamin A-active form, retinoic acid (RA) in the regulation of several developmental genes, including the important growth regulatory factor, transforming growth factor-beta2 (TGFβ2), involved in early events of heart morphogenesis. However, this in ovo model is not readily available for elucidating details of molecular mechanisms determining RA activity, thus limiting further examination o…

Nutritional, chemical, syneresis, sensory properties, and shelf life of Iranian traditional yoghurts during storage

2019

Tuluq and Torba yoghurts are traditional concentrates from Iran. Physicochemical, nutritional, and sensory properties of these yoghurts were studied along 60 days of storage. Results showed that, both pH and percentage of free whey decreased significantly (P < 0.05), while titratable acidity, total solid, salt, protein and fat content increased (P < 0.05) during storage. The yoghurt lipolysis decreased during the first 30 days and then increased during the storage. The indexes pH 4.6-soluble nitrogen/total nitrogen and non-protein nitrogen/total nitrogen in yoghurt samples decreased during first 30 days, possibly due to removing of low molecular weight nitrogenous compounds of Tuluq and Tor…

Conical nanopores highlight the pro-aggregating effects of pyrimethanil fungicide on Aβ(1-42) peptides and dimeric splitting phenomena.

2022

International audience; The Aβ(1-42) aggregation is a key event in the physiopathology of Alzheimer's disease (AD). Exogenous factors such as environmental pollutants, and more particularly pesticides, can corrupt Aβ(1-42) assembly and could influence the occurrence and pathophysiology of AD. However, pesticide involvement in the early stages of Aβ(1-42) aggregation is still unknown. Here, we employed conical track-etched nanopore in order to analyse the Aβ(1-42) fibril formation in the presence of pyrimethanil, a widely used fungicide belonging to the anilinopyrimidine class. Our results evidenced a pro-aggregating effect of pyrimethanil on Aβ(1-42). Aβ(1-42) assemblies were successfully d…

The Binomial “Inflammation-Epigenetics” in Breast Cancer Progression and Bone Metastasis: IL-1β Actions Are Influenced by TET Inhibitor in MCF-7 Cell…

2022

The existence of a tight relationship between inflammation and epigenetics that in primary breast tumor cells can lead to tumor progression and the formation of bone metastases was investigated. It was highlighted how the induction of tumor progression and bone metastasis by Interleukin-1 beta, in a non-metastatic breast cancer cell line, MCF-7, was dependent on the de-methylating actions of ten-eleven translocation proteins (TETs). In fact, the inhibition of their activity by the Bobcat339 molecule, an inhibitor of TET enzymes, determined on the one hand, the modulation of the epithelial-mesenchymal transition process, and on the other hand, the reduction in the expression of markers of bo…

Sequence similarity of mammalian epoxide hydrolases to the bacterial haloalkane dehalogenase and other related proteins Implication for the potential…

1994

Direct comparison of the amino acid sequences of microsomal and soluble epoxide hydrolase superficially indicates that these enzymes are unrelated. Both proteins, however, share significant sequence similarity to a bacterial haloalkane dehalogenase that has earlier been shown to belong to the alpha/beta hydrolase fold family of enzymes. The catalytic mechanism for the dehalogenase has been elucidated in detail [Verschueren et al. (1993) Nature 363, 693-698] and proceeds via an ester intermediate where the substrate is covalently bound to the enzyme. From these observations we conclude (i) that microsomal and soluble epoxide hydrolase are distantly related enzymes that have evolved from a co…

Chemical composition of the essential oil from different vegetative parts of Foeniculum vulgare subsp. piperitum (Ucria) Coutinho (Umbelliferae) grow…

2021

In the present study, the chemical compositions of the essential oils from roots, stems, leaves and fruits of Foeniculum vulgare subsp. piperitum collected in Sicily were evaluated by GC and GC-MS. The main components of the roots were terpinolene (33.15%), gamma-terpinene (12.18%) and fenchyl acetate (11.23%). Stems and leaves were very rich in alpha-phellandrene (36.85% and 41.59%, respectively) and beta-phellandrene (19.68% and 25.79%, respectively), whereas the main components of fruits were terpinolene (20.10%) and limonene (17.84%)These results were compared with those of the EOs of the same vegetative parts of Foeniculum vulgare subsp. vulgare, collected in the same station and in th…

Effect of freezing/thawing process in different sizes of blue fish in the Mediterranean through lysosomal enzymatic tests

2014

The assessment of freshness of different sizes of blue fish (Engraulis encrasicolus 12 cm, Sardina pilchardus 15 cm, Trachurus trachurus 40 cm, Scomber japonicus colias 60 cm) was carried out using non-conventional enzymatic methods. The activities of the three lysosomal enzymes (α-glucosidase (AG), β-galactosidase (B-GAL) and β-N-acetylglucosamidase (B-NA)) in extracts of blue fish muscle were measured over a period of 21 days of storage. A significant increase (p < 0.05) of AG activity was observed in all species, with a large increase seen after only one day of storage. B-NA activity increased slightly in sardines, horse mackerels and chub mackerel during frozen/thawed storage. Finally, …

Editorial Comment from Dr Michel to Expression and functional role of β3-adrenoceptors in the human ureter

2013

Distribución, función y vías de señalización de los adrenoceptores β a nivel vascular

2014

Se ha analizado la contribución de los diferentes subtipos de adrenoceptores (AR) β (β1, β2 β3) en la vasodilatación, determinando su localización en las diferentes células vasculares y evaluando la implicación de las vías de señalización adenilato ciclasa (AC)/AMPc/PKA y/o NO/guanilato ciclasa soluble (GCs)/GMPc, en dos lechos vasculares representantes de: los vasos de resistencia, como la arteria mesentérica de resistencia (A.M.R.) de rata, y de conductancia, como la aorta de rata. Los resultados obtenidos muestran que, a pesar de que los tres subtipos de AR β se expresan en ambos vasos, su participación en la respuesta funcional, así como la vía de señalización utilizada, es diferente se…