Search results for "11b"
showing 10 items of 43 documents
Glioblastoma cells induce differential glutamatergic gene expressions in human tumor-associated microglia/macrophages and monocyte-derived macrophages
2015
Glioblastoma cells produce and release high amounts of glutamate into the extracellular milieu and subsequently can trigger seizure in patients. Tumor-associated microglia/macrophages (TAMs), consisting of both parenchymal microglia and monocytes-derived macrophages (MDMs) recruited from the blood, are known to populate up to 1/3 of the glioblastoma tumor environment and exhibit an alternative, tumor-promoting and supporting phenotype. However, it is unknown how TAMs respond to the excess extracellular glutamate in the glioblastoma microenvironment. We investigated the expressions of genes related to glutamate transport and metabolism in human TAMs freshly isolated from glioblastoma resecti…
Mast Cell–deficient KitW-sh “Sash” Mutant Mice Display Aberrant Myelopoiesis Leading to the Accumulation of Splenocytes That Act as Myeloid-Derived S…
2013
Abstract Mast cell-deficient KitW-sh “sash” mice are widely used to investigate mast cell functions. However, mutations of c-Kit also affect additional cells of hematopoietic and nonimmune origin. In this study, we demonstrate that KitW-sh causes aberrant extramedullary myelopoiesis characterized by the expansion of immature lineage-negative cells, common myeloid progenitors, and granulocyte/macrophage progenitors in the spleen. A consistent feature shared by these cell types is the reduced expression of c-Kit. Populations expressing intermediate and high levels of Ly6G, a component of the myeloid differentiation Ag Gr-1, are also highly expanded in the spleen of sash mice. These cells are …
IL-23-mediated mononuclear phagocyte crosstalk protects mice from Citrobacter rodentium-induced colon immunopathology.
2014
Gut homeostasis and mucosal immune defense rely on the differential contributions of dendritic cells (DC) and macrophages. Here we show that colonic CX3CR1+ mononuclear phagocytes are critical inducers of the innate response to Citrobacter rodentium infection. Specifically, the absence of IL-23 expression in macrophages or CD11b+ DC results in the impairment of IL-22 production and in acute lethality. Highlighting immunopathology as a death cause, infected animals are rescued by the neutralization of IL-12 or IFNγ. Moreover, mice are also protected when the CD103+ CD11b− DC compartment is rendered deficient for IL-12 production. We show that IL-12 production by colonic CD103+ CD11b− DC is r…
CCDC 894411: Experimental Crystal Structure Determination
2013
Related Article: S.Fustero, P.Bello, J.Miro, M.Sanchez-Rosello, M.A.Maestro, J.Gonzalez, C.del Pozo|2013|Chem.Commun.|49|1336|doi:10.1039/c2cc37796a
Do Current Domestic Gigabit Wireless Technologies Fulfill User Requirements for Ultra High Definition Videos?
2017
We present the results from a measurement-based performance evaluation of wireless networks based on IEEE 802.11n and IEEE 802.11ac standards in an indoor environment, with the aim to analyze their performance under high definition streaming video applications. We focus our study on analyzing the highest performance of these standards using off-the-shelf equipment, both with synthetic TCP and UDP traffic to measure the saturation throughput as well as high definition video streams. The measurements have been conducted in the student labs of our university and show good performance for streaming purposes in high definition and also ultra-high definition from a subjective video quality point …
Assessing the effectiveness of IEEE 802.11e in multi-hop mobile network environments
2004
The IEEE 802.11e technology is receiving much interest due to the promising enhancements it offers to wireless local area networks in terms of QoS support. Until now, research has focused on single hop, access point based environments, which are the most common. We review the enhancements proposed in the last IEEE 802.11e draft (version 8.0). We analyze the performance of the IEEE 802.11e protocol on ad-hoc networks (multi-hop) in terms of throughput and end-to-end delay. We also measure the effectiveness for static and fully dynamic networks with a variable number of source stations. Finally, we analyze the performance of IEEE 802.11e when legacy IEEE 802.11 stations (no IEEE 802.11e suppo…
Performance Study of Non-beaconed and Beacon-Enabled Modes in IEEE 802.15.4 under Bluetooth Interference
2008
The IEEE 802.15.4 protocol is a flexible technology with great potential for wireless sensor networks applications. The standard distinguishes two network modes; beacon-enabled and nonbeacon-enabled. In this paper, the performance study of beaconed and non-beaconed modes of IEEE 802.15.4 is evaluated using on site PER measurements. The tests are carryout in an urban outdoor environment with commercial available equipment. Likewise, the impact of Bluetooth interference over IEEE 802.15.4 modes is evaluated. The results show that Bluetooth interference and the mode selected have effects over the PER. Furthermore, for distances between 3 to 10 meters the PER doesn't show significance distance …
A role for Toll-like receptor mediated signals in neutrophils in the pathogenesis of the anti-phospholipid syndrome.
2012
The anti-phospholipid syndrome (APS) is characterized by recurrent thrombosis and occurrence of anti-phospholipid antibodies (aPL). aPL are necessary, but not sufficient for the clinical manifestations of APS. Growing evidence suggests a role of innate immune cells, in particular polymorphonuclear neutrophils (PMN) and Toll-like receptors (TLR) to be additionally involved. aPL activate endothelial cells and monocytes through a TLR4-dependent signalling pathway. Whether this is also relevant for PMN in a similar way is currently not known. To address this issue, we used purified PMN from healthy donors and stimulated them in the presence or absence of human monoclonal aPL and the TLR4 agonis…
Polymorphonuclear leukocyte integrins in chronic renal failure
2005
Patients with chronic renal failure (CRF), in comparison with general population, show a higher cardiovascular mortality, not fully explained by the "traditional" risk factors. Among the new factors that have been hypothesized, leukocytes might play an important role. In a group of patients with mild CRF we determined, at baseline and after in vitro activation with 4-phorbol-12-myristate-13-acetate (PMA) and N-formyl-methionyl-leucyl-phenylalanine (fMLP), the polymorphonuclear leukocytes (PMN) beta2-integrin pattern (CD11a, CD11b, CD11c and CD18) by using indirect immunofluorescence with a flow cytometer. At baseline we observed an increase in the phenotypical expression of CD11b, CD11c and…
Migration of CD11b+ accessory cells during murine lung regeneration
2013
In many mammalian species, the removal of one lung leads to growth of the remaining lung to near-baseline levels. In studying post-pneumonectomy mice, we used morphometric measures to demonstrate neoalveolarization within 21 days of pneumonectomy. Of note, the detailed histology during this period demonstrated no significant pulmonary inflammation. To identify occult blood-borne cells, we used a parabiotic model (wild-type/GFP) of post-pneumonectomy lung growth. Flow cytometry of post-pneumonectomy lung digests demonstrated a rapid increase in the number of cells expressing the hematopoietic membrane molecule CD11b; 64.5% of the entire GFP(+) population were CD11b(+). Fluorescence microscop…