Search results for "16S"
showing 10 items of 464 documents
Adaptative biochemical pathways and regulatory networks in Klebsiella oxytoca BAS-10 producing a biotechnologically relevant exopolysaccharide during…
2012
Abstract Background A bacterial strain previously isolated from pyrite mine drainage and named BAS-10 was tentatively identified as Klebsiella oxytoca. Unlikely other enterobacteria, BAS-10 is able to grow on Fe(III)-citrate as sole carbon and energy source, yielding acetic acid and CO2 coupled with Fe(III) reduction to Fe(II) and showing unusual physiological characteristics. In fact, under this growth condition, BAS-10 produces an exopolysaccharide (EPS) having a high rhamnose content and metal-binding properties, whose biotechnological applications were proven as very relevant. Results Further phylogenetic analysis, based on 16S rDNA sequence, definitively confirmed that BAS-10 belongs t…
Thalassobacter stenotrophicus gen. nov., sp. nov., a novel marine alpha-proteobacterium isolated from Mediterranean sea water.
2005
A Gram-negative, slightly halophilic, strictly aerobic, chemo-organotrophic bacterium was isolated from Mediterranean sea water near Valencia (Spain). 16S rRNA gene sequence comparisons showed that the isolate represented a separate branch within the α-3 subclass of the Proteobacteria, now included within the order ‘Rhodobacterales’. Jannaschia helgolandensis was the closest relative, but their low sequence similarity and other features indicated that they were not related at the genus level. Isolate 5SM22T produced bacteriochlorophyll a and grew on solid media as regular salmon-pink colonies. Cells are motile rods, with polar flagella. The DNA G+C content is 59·1 mol%. Morphological, physi…
Análisis poblacional y filogeográfico de vectores de la enfermedad de Chagas en Perú basado en análisis multigénicos del ADN ribosomal y mitocondrial
2017
La enfermedad de Chagas, cuyo agente causal es Trypanosoma cruzi, constituye un grave problema de salud pública en toda Latinoamérica. La principal forma de transmisión es la vectorial, de la que son responsables hemípteros redúvidos de la subfamilia Triatominae adaptados a los hábitats doméstico y peridoméstico. Puesto que el parásito se mantiene en un ciclo zoonótico silvestre y es imposible de eliminar, la principal forma de lucha contra la transmisión vectorial consiste en la eliminación de las poblaciones domiciliadas del vector. Los estudios moleculares constituyen una herramienta de gran utilidad a la hora de planificar las campañas de control vectorial, puesto que permiten conocer y…
A New Niche for Anoxygenic Phototrophs as Endoliths
2018
ABSTRACT Anoxygenic phototrophic bacteria (APBs) occur in a wide range of aquatic habitats, from hot springs to freshwater lakes and intertidal microbial mats. Here, we report the discovery of a novel niche for APBs: endoliths within marine littoral carbonates. In a study of 40 locations around Isla de Mona, Puerto Rico, and Menorca, Spain, 16S rRNA high-throughput sequencing of endolithic community DNA revealed the presence of abundant phylotypes potentially belonging to well-known APB clades. An ad hoc phylogenetic classification of these sequences enabled us to refine the assignments more stringently. Even then, all locations contained such putative APBs, often reaching a significant pro…
16S rRNA sequences as diagnostic tools to elucidate potential symbiotic relationships between bacteria and the marine sponge Halichondria panicea : r…
1999
New insights into the gut microbiome in loggerhead sea turtles Caretta caretta stranded on the Mediterranean coast
2019
Caretta caretta is the most common sea turtle species in the Mediterranean Sea. The species is threatened by anthropomorphic activity that causes thousands of deaths and hundreds of strandings along the Mediterranean coast. Stranded turtles are often cared for in rehabilitation centres until they recover or die. The objective of this study was to characterize the gut microbiome of nine sea turtles stranded along the Sicilian coast of the Mediterranean Sea using high-throughput sequencing analysis targeting V3–V4 regions of the bacterial 16S rRNA gene. Stool samples were collected from eight specimens hosted in the recovery centre after a few days of hospitalization (under 7) and from one ho…
Mapping and determinism of soil microbial community distribution across an agricultural landscape.
2015
Article en open access; International audience; Despite the relevance of landscape, regarding the spatial patterning of microbial communities and the relative influence of environmental parameters versus human activities, few investigations have been conducted at this scale. Here, we used a systematic grid to characterize the distribution of soil microbial communities at 278 sites across a monitored agricultural landscape of 13km(2). Molecular microbial biomass was estimated by soil DNA recovery and bacterial diversity by 16S rRNA gene pyrosequencing. Geostatistics provided the first maps of microbial community at this scale and revealed a heterogeneous but spatially structured distribution…
Different Medicago truncatula genotypes, expressing different phenotypes, modulate microbial functional genes in the rhizosphere
2022
Plants host in their rhizosphere a remarkable diversity of microorganisms that in return promote plant growth and health. Thus, the plant microbiota emerges as a novel component that expand the capacity of plants to adapt to the environment, and thus pave the way for future breeding approaches.It is widely known that different plant species harbor different microbiota, but very few studies showed an impact of the plant genotypes on the microbiota. These contrasting results may depend on the choice of the tested genotypes.We formulate the hypothesis that only those plant genotypes showing high genetic diversity and expressing different phenotypes harbor different rhizosphere microbiota and a…
Estudio transversal de la microbiología salival mediante pirosecuenciación en escolares de la Comunitat Valenciana y su relación con la caries dental
2013
La salud oral, que está fuertemente influenciada por la microbiota oral, ejerce un papel significativo en la salud general. Las comunidades bacterianas orales contienen especies que promueven estados de salud, mientras que otras contribuyen a la enfermedad. Conocer qué especies están presentes y cómo se componen las comunidades microbianas en estados de salud es el primer paso para comprender qué cambios pueden llevar a la enfermedad. El tipo de muestras a utilizar para este tipo de estudios, bien con objetivos epidemiológicos o etiológicos, es crucial. La saliva representa un medio fácil y no invasivo de obtención de muestras que contengan bacterias orales de varias localizaciones como las…
Cilvēka asins mikrobioma kompozīcijas, stabilitātes un izcelsmes noteikšana veselos indivīdos
2021
Neseni pētījumi ir apliecinājuši asins mikrobioma klātesamību veselos cilvēkos, tomēr tā izcelsme vēl ir neskaidra. Maģistra darba mērķis bija noskaidrot asins mikrobioma kompozīciju, potenciālo izcelsmi un dinamiku laika gaitā veseliem indivīdiem. Asins mikrobioma izcelsmes noskaidrošanai 10 veseliem indivīdiem ievāca pilnasins paraugus un ādas, maksts, mutes un fēču mikrobioma uztriepes. Pilnasins un fēču paraugus ievāca no diviem laika punktiem ar 3 mēnešu intervālu. Ar 16S rRNS gēna V3-V4 reģiona analīzi noteica to taksonomisko sastāvu. Noskaidrojās, ka asins mikrobioms ir unikāls - tajā izteikti dominē Proteobacteria tipa Pseudomonas ģints pārstāvji, un vienlaikus tā potenciālā izcelsm…