Search results for "16S"
showing 10 items of 464 documents
Methylophaga and Hyphomicrobium can be used as target genera in monitoring saline water methanol-utilizing denitrification.
2016
Abstract Which bacterial taxonomic groups can be used in monitoring saline water methanol-utilizing denitrification and whether nitrate is transformed into N2 in the process are unclear. Therefore, methylotrophic bacterial communities of two efficiently functioning (nitrate/nitrite reduction was 63–96 %) tropical and cool seawater reactors at a public aquarium were investigated with clone library analysis and 454 pyrosequencing of the 16S rRNA genes. Transformation of nitrate into N2 was confirmed using 15N labeling in incubation of carrier material from the tropical reactor. Combining the data with previous study results, Methylophaga and Hyphomicrobium were determined to be suitable targe…
The Monoclonal Antitoxin Antibodies (Actoxumab–Bezlotoxumab) Treatment Facilitates Normalization of the Gut Microbiota of Mice with Clostridium diffi…
2016
Antibiotics have significant and long-lasting impacts on the intestinal microbiota and consequently reduce colonization resistance against Clostridium difficile infection (CDI). Standard therapy using antibiotics is associated with a high rate of disease recurrence, highlighting the need for novel treatment strategies that target toxins, the major virulence factors, rather than the organism itself. Human monoclonal antibodies MK-3415A (actoxumab–bezlotoxumab) to C. difficile toxin A and toxin B, as an emerging non-antibiotic approach, significantly reduced the recurrence of CDI in animal models and human clinical trials. Although the main mechanism of protection is through direct neutraliza…
Isolation and characterisation of new Gram-negative and Gram-positive atrazine degrading bacteria from different French soils
2001
The capacity of 12 soils to degrade atrazine was studied in laboratory incubations using radiolabelled atrazine. Eight soils showed enhanced degradation of this compound. Twenty-five bacterial strains able to degrade atrazine were isolated by an enrichment method from 10 of these soils. These soils were chosen for their wide range of physico-chemical characteristics. Their history of treatment with atrazine was also variable. The genetic diversity of atrazine degraders was determined by amplified ribosomal restriction analysis (ARDRA) of the 16S rDNA gene with three restriction endonucleases. The 25 bacterial strains were grouped into five ARDRA types. By sequencing and aligning the 16S rDN…
Diversità dei procarioti in acque di falda contaminate da solventi clorurati
2021
Host’s genetic background determines the outcome of reciprocal faecal transplantation on life-history traits and microbiome composition
2022
Abstract Background Microbes play a role in their host's fundamental ecological, chemical, and physiological processes. Host life-history traits from defence to growth are therefore determined not only by the abiotic environment and genotype but also by microbiota composition. However, the relative importance and interactive effects of these factors may vary between organisms. Such connections remain particularly elusive in Lepidoptera, which have been argued to lack a permanent microbiome and have microbiota primarily determined by their diet and environment. We tested the microbiome specificity and its influence on life-history traits of two colour genotypes of the wood tiger moth (Arctia…
Old sleeping Sicilian beauty: seed germination in the palaeoendemic Petagnaea gussonei (Spreng.) Rauschert (Saniculoideae, Apiaceae).
2015
Petagnaea gussonei (Apiaceae) is a perennial herbaceous species endemic to northeast Sicily (Nebrodi Mountains). It is considered a remnant of the Sicilian Tertiary flora, and is endangered according to the Red List. There is no information in the literature about the germinability of its seeds, even though seed production is know to occur. The aim of this study was to obtain data to better understand seed germination of this species and its biological implications. Thus, several approaches were employed: vitality analyses, gibberellic acid supply, germination and soil microbial flora analyses via end-point and qPCR. The results suggest that seed germination occurs after ca. 1.5 years at a …
Fēču mikrobiotas raksturojums un to ietekmējošie faktori bērniem
2020
Ievads. Agrīna mikrobiotas attīstība ietekmē gan imūnsistēmas nobriešanu, gan dažādu patoloģiju, kā, piemēram, alerģijas, infekciju un citu slimību attīstību bērnībā, tāpēc nepieciešams identificēt mikrobiotas sastāva atšķirības un to ietekmējošos agrīnos riska faktorus bērniem. Darba mērķis. Pētīt fēču mikrobiotas sastāvu pirmsskolas vecuma bērniem un analizēt to ietekmējošos faktorus. Materiāli un metodes. Šķērsgriezuma pētījums veikts primārajos veselības aprūpes centros. Bērnu (līdz piecu gadu vecumam) vecākiem tika lūgts aizpildīt anketu (demogrāfiskie dati, dzimšanas veids, barošana pirmajā dzīves gadā, antibakteriāla terapija mātei laktācijas laikā, ģimenes dati) un atnest bērna fēču…
Recurrent inoculation: a strategy for a better survival of Pseudomonas fluorescens strain in soil
2022
A growing interest is shown for microbial inoculants as a promising alternative to agrochemicals for sustainable agricultural. However, biotic and abiotic stresses are known to affect their establishment and effectiveness, leading to inconsistent performance in field conditions. At the same time, a poor survival of the strain is often observed, which constitutes a main hamper for bacterial inoculant development. We hypothesize that recurrent inoculations would improve the survival of the strain in soil. For this purpose, we tested different scenarios of inoculation of the plant growth promoting rhizobacteria Pseudomonas fluorescens in soil microcosms: a single inoculation, two inoculations …
Stability of the human microbiome. Health and disease imprinted in the time variability of the human microbiota.
2019
Animal microbiota plays an essential role in keeping the physiological status of the host healthy. This microbiota consists of hundreds of different species that inhabit the host, and whose composition varies on a daily basis due to multiple factors as diets, medicine intakes, lifestyle or diseases. It is a highly dynamical system that shows variability even when the host is in a healthy condition, but visible changes happen when the system is perturbed. Thus, the main objective of this thesis aimed to study the temporal variability of human microbiota, and link it to the health status of the host. For this, we proposed a formal definition of stability in microbial systems based on the use …
Amoksicilīna un klaritromicīna kombinētās terapijas ilgtermiņa ietekme uz zarnu trakta mikrobiomu
2018
Helicobacter pylori ir viens no pirmajiem identificētajiem bakteriālajiem karcinogēniem un Eiropas medicīnas pārstāvju izstrādātās vadlīnijas iekļauj eradikācijas shēmu, kurā ietilpst kombinēta plaša spektra antibiotiku pielietošana. Antibakteriālā terapija, it īpaši plaša spektra antibiotiku pielietošana rada risku īstermiņa un ilgtermiņa zarnu trakta mikrobioma disbiozes attīstībai. Maģistra darba mērķis bija noskaidrot Helicobacter pylori kombinētās antibakteriālās terapijas izraisītās ilgtermiņa izmaiņas zarnu trakta mikrobiomā. Pētījuma gaitā tika veikta 45 pacientu 16s rRNS amplikonu analīze, salīdzinot pirms un pēc terapijas iegūtos fēču paraugus. Ilgtermiņa antibakteriālās terapijas…