Search results for "22"

showing 10 items of 13984 documents

Reply to Comment on: 'Corneal confocal scanning laser microscopy in patients with dry eye disease treated with topical cyclosporine'

2017

Reply to Comment on: ‘Corneal confocal scanning laser microscopy in patients with dry eye disease treated with topical cyclosporine’

0301 basic medicineLaser Microscopymedicine.medical_specialtygenetic structuresConfocalAdministration TopicaleducationDry Eye SyndromesOphthalmic SolutionCornea03 medical and health sciencesImmunosuppressive Agent0302 clinical medicineOphthalmologyCorneaMicroscopyCorrespondenceMedicineHumansIn patientOphthalmology; Sensory Systems; Dry Eye; Corneal confocal scanning laser microscopyCorneal confocal scanning laser microscopyMicroscopy Confocalbusiness.industryDry Eyeeye diseasesSensory SystemsOphthalmology030104 developmental biologyOphthalmic solutionsmedicine.anatomical_structure030221 ophthalmology & optometryCyclosporineDry Eye Syndromessense organsOphthalmic SolutionsbusinessImmunosuppressive AgentsHuman
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Changing the education paradigm in oncology: ESO masterclass, 17 years of continuous success

2020

In this review, we summarize the history of the 41 Masterclasses in Clinical Oncology (MCO) organized by ESO or ESO-ESMO during the last 17 years. MCOs have been held in five different geographical regions including: a) Central Europe, b) Eastern Europe and Balkans, c) Baltic and Euroasia, d) Arab World and Southern European Countries and e) Latin America. More than 2.000 young oncologists have attended and more than 250 distinguished faculty members have actively participated. The program exposes students to sessions covering all major tumors ("big killers") and to spotlights updating information on various important cancers and related topics. Participants are able to present their own cl…

0301 basic medicineLatin Americans2720 HematologyLearning assessment610 Medicine & healthMedical Oncology03 medical and health sciences0302 clinical medicineSurveys and QuestionnairesMedicineTumor boardHumansSocieties MedicalClinical OncologyOncologistsMedical educationbusiness.industryTeachingHematologyTest (assessment)Europe030104 developmental biologyOncologyEducation Medical Graduate030220 oncology & carcinogenesis10032 Clinic for Oncology and Hematology2730 OncologyClinical casebusiness
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Risk factors for anastomotic leakage after colorectal resection in ovarian cancer surgery: A multi-centre study.

2019

Objective. To determine pre-/intraoperative risk factors for anastomotic leak after modified posterior pelvic exenteration (MPE) or colorectal resection in ovarian cancer and to create a practical instrument for predicting anastomotic leak risk. Background. In advanced ovarian cancer surgery, there is rather limited published evidence, drawn from a small sample, providing information about risk factors for anastomotic leak. Methods. Eight hospitals participated in this retrospective study. Data on 695 patients operated for ovarian cancer with primary anastomosis were included (January 2010-June 2018). Twelve pre-/intraoperative variables were analysed as potential independent risk factors f…

0301 basic medicineLeakmedicine.medical_specialtyMultivariate analysisAnastomotic LeakAnastomosisStoma03 medical and health sciences0302 clinical medicineOvarian cancerRetrospective StudieRisk FactorsIntestine SmallmedicineAnastomotic leakHumansAge FactorColectomySerum AlbuminAgedRetrospective StudiesOvarian NeoplasmsProctectomybusiness.industryIncidence (epidemiology)Ovarian NeoplasmRisk FactorAnastomosis SurgicalSuture TechniquesAge FactorsObstetrics and GynecologyRetrospective cohort studyMiddle Agedmedicine.diseasePosterior Pelvic ExenterationSurgeryPelvic Exenteration030104 developmental biologyRisk factorsOncology030220 oncology & carcinogenesisFemaleNeoplasm Recurrence LocalOvarian cancerbusinessHumanGynecologic oncology
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Phenolic extract from oleaster (Olea europaea var. Sylvestris) leaves reduces colon cancer growth and induces caspase-dependent apoptosis in colon ca…

2017

Erratum inCorrection: Phenolic extract from oleaster (Olea europaea var. Sylvestris) leaves reduces colon cancer growth and induces caspase-dependent apoptosis in colon cancer cells via the mitochondrial apoptotic pathway. [PLoS One. 2017]; International audience; Dietary polyphenols, derived from natural products, have received a great interest for their chemopreventive properties against cancer. In this study, we investigated the effects of phenolic extract of the oleaster leaves (PEOL) on tumor growth in mouse model and on cell death in colon cancer cell lines. We assessed the effect of oleaster leaf infusion on HCT116 (human colon cancer cell line) xenograft growth in athymic nude mice.…

0301 basic medicineLeavesCarcinoma Cellslcsh:MedicineApoptosisPlant ScienceMitochondrionEndoplasmic ReticulumBiochemistry[ SDV.CAN ] Life Sciences [q-bio]/Cancer0302 clinical medicineMedicine and Health SciencesMitochondrial calcium uptakeDiseaselcsh:ScienceEnergy-Producing OrganellesStainingchemistry.chemical_classificationSecretory PathwayMultidisciplinaryCell DeathPlant AnatomyCytochrome cCell StainingAnimal ModelsMitochondriaOlive Leaf ExtractChemistryOncologyExperimental Organism SystemsBiochemistryCell Processes030220 oncology & carcinogenesisPhysical SciencesCellular Structures and OrganellesResearch ArticleProgrammed cell deathActivationMouse Models[SDV.CAN]Life Sciences [q-bio]/CancerBioenergeticsBiologyResearch and Analysis MethodsColorectal-CancerCaspase-Dependent Apoptosis03 medical and health sciencesModel OrganismsPhenolsCytochrome-CColorectal CancerReactive oxygen speciesP53Cell growthProteinlcsh:RChemical CompoundsBiology and Life SciencesCancers and NeoplasmsPolyphenolsCell BiologyMolecular biology030104 developmental biologychemistrySpecimen Preparation and TreatmentApoptosisbiology.proteinCalciumlcsh:QPLoS ONE
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Trabectedin for patients with advanced soft tissue sarcoma: A non-interventional, retrospective, multicenter study of the italian sarcoma group

2021

The Italian Sarcoma Group performed this retrospective analysis of patients with advanced soft tissue sarcoma, pretreated with ≥1 anthracycline-based treatment, and treated with trabectedin every three weeks. Primary endpoint was to describe real-life use of trabectedin across Italy. Secondary endpoints included objective response rate (ORR) and safety. Overall, 512 patients from 20 Italian centers were evaluated. Leiomyosarcoma (37.7%)/liposarcoma (30.3%) were the most prevalent histological types (abbreviated as L-sarcoma). Patients received a median of four trabectedin cycles (range: 1–40), mostly as a second-line treatment (~60% of patients). The ORR was 13.7% superior (p &lt

0301 basic medicineLeiomyosarcomaCancer Researchmedicine.medical_specialtyDacarbazinelcsh:RC254-282GastroenterologyArticlePazopanib03 medical and health sciences0302 clinical medicineInternal medicinemedicineProgression-free survivalObservationalTrabectedinSoft tissue sarcomaPerformance statusbusiness.industrySoft tissue sarcomaReal-lifelcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogensmedicine.disease030104 developmental biologyOncology030220 oncology & carcinogenesisSarcomabusinessmedicine.drugTrabectedin
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Deep learning for diagnosis and survival prediction in soft tissue sarcoma.

2021

Background Clinical management of soft tissue sarcoma (STS) is particularly challenging. Here, we used digital pathology and deep learning (DL) for diagnosis and prognosis prediction of STS. Patients and methods Our retrospective, multicenter study included a total of 506 histopathological slides from 291 patients with STS. The Cancer Genome Atlas cohort (240 patients) served as training and validation set. A second, multicenter cohort (51 patients) served as an additional test set. The use of the DL model (DLM) as a clinical decision support system was evaluated by nine pathologists with different levels of expertise. For prognosis prediction, 139 slides from 85 patients with leiomyosarcom…

0301 basic medicineLeiomyosarcomamedicine.medical_specialtySoft Tissue Neoplasms03 medical and health sciences0302 clinical medicineDeep LearningmedicineHumansRetrospective StudiesReceiver operating characteristicProportional hazards modelbusiness.industrySoft tissue sarcomaHazard ratioDigital pathologySarcomaHematologymedicine.diseasePrognosisConfidence interval030104 developmental biologyOncology030220 oncology & carcinogenesisCohortRadiologybusinessAnnals of oncology : official journal of the European Society for Medical Oncology
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Cytotoxicity of South-African medicinal plants towards sensitive and multidrug-resistant cancer cells

2016

Abstract Ethnopharmacological relevance Traditional medicine plays a major role for primary health care worldwide. Cancer belongs to the leading disease burden in industrialized and developing countries. Successful cancer therapy is hampered by the development of resistance towards established anticancer drugs. Aim In the present study, we investigated the cytotoxicity of 29 extracts from 26 medicinal plants of South-Africa against leukemia cell lines, most of which are used traditionally to treat cancer and related symptoms. Material and methods We have investigated the plant extracts for their cytotoxic activity towards drug-sensitive parental CCRF-CEM leukemia cells and their multidrug-r…

0301 basic medicineLeonotis leonurusCell SurvivalPlectranthusPharmacologyMagnoliopsidaSouth Africa03 medical and health sciences0302 clinical medicineCell Line TumorNeoplasmsDrug DiscoveryPlectranthus barbatusmedicineHumansMedicinal plantsMedicine African TraditionalPharmacologyLeonurusPlants MedicinalbiologyPlant ExtractsCancerbiology.organism_classificationmedicine.diseaseAntineoplastic Agents PhytogenicDrug Resistance MultipleLeukemia030104 developmental biologyDrug Resistance Neoplasm030220 oncology & carcinogenesisCancer cellJournal of Ethnopharmacology
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Early endocrine disruptors exposure acts on 3T3-L1 differentiation and endocrine activity

2017

International audience; Introduction: Data from last years suggested that early exposure to endocrine disruptors (EDs) can predispose newborns to endocrine dysfunction of adipocytes, obesity, and associated disorders. The implication of EDs at low doses on adipocyte development has been poorly investigated. For instance, vinclozolin (V) is a dicarboximide fungicide widely used in agriculture since the 90's, alone or in mixture with genistein (G), an isoflavonoid from Leguminosae. This study aims to identify the effect of vinclozolin alone or with genistein, on adipose tissue properties using cell culture.Methods: In steroid-free conditions, 3T3-L1 pre-adipocytes were induced to differentiat…

0301 basic medicineLeptin[ SDV.TOX.ECO ] Life Sciences [q-bio]/Toxicology/Ecotoxicologytissu adipeuxPharmaceutical ScienceGenisteinAdipose tissueoestradiolsourisTriglyceridechemistry.chemical_compound0302 clinical medicineAdipocyteratVinclozolinlcsh:QH301-705.5Original Researchperturbateur endocrinienlcsh:R5-920LeptinGeneral MedicineGenistein[ SDV.TOX.TCA ] Life Sciences [q-bio]/Toxicology/Toxicology and food chainobésité030220 oncology & carcinogenesisAlimentation et NutritionEndocrinologie et métabolismeleptine[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicologylcsh:Medicine (General)medicine.medical_specialtyAdipose tissue[SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chainBiologyadipocyteGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencesInternal medicinemedicineFood and NutritionEndocrine systemEndocrine disruptorsEndocrinology and metabolism3T3-L1adipose tissue;endocrine disruptors;genistein;triglyceride;leptin;vinclozolin;promotes adipocyte differentiation;adipose-tissue development;rat;adipocytes;mice;obesity;expression;estradiol030104 developmental biologyEndocrinologylcsh:Biology (General)chemistryVinclozolinCell culture
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TargetPlex FFPE-Direct DNA Library Preparation Kit for SiRe NGS panel: An international performance evaluation study

2021

AimNext generation sequencing (NGS) represents a key diagnostic tool to identify clinically relevant gene alterations for treatment-decision making in cancer care. However, the complex manual workflow required for NGS has limited its implementation in routine clinical practice. In this worldwide study, we validated the clinical performance of the TargetPlex FFPE-Direct DNA Library Preparation Kit for NGS analysis. Impressively, this new assay obviates the need for separate, labour intensive and time-consuming pre-analytical steps of DNA extraction, purification and isolation from formalin-fixed paraffin embedded (FFPE) specimens in the NGS workflow.MethodsThe TargetPlex FFPE-Direct DNA Libr…

0301 basic medicineLibraryComputer scienceGenomicsComputational biologylung neoplasmsDNA sequencingPathology and Forensic Medicine03 medical and health sciences0302 clinical medicineHumansmolecular biologymolecularbiomarkers; lung neoplasms; molecular; molecular biology; pathology; tumourGene LibraryParaffin EmbeddingtumourSireClinical performancebiomarkersHigh-Throughput Nucleotide SequencingGeneral MedicineGenomicsDNA extractionParaffin embeddedlung neoplasm030104 developmental biologyWorkflow030220 oncology & carcinogenesisMutationbiomarkerpathology
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Modeling the Hematopoietic Landscape

2019

Some time ago, we proposed a continuum-like view of the lineages open to hematopoietic stem cells (HSCs); each HSC self-renews or chooses from the spectrum of all end-cell options and can then “merely” differentiate. Having selected a cell lineage, an individual HSC may still “step sideways” to an alternative, albeit closely related, fate: HSC and their progeny therefore remain versatile. The hematopoietic cytokines erythropoietin, granulocyte colony-stimulating factor, macrophage colony-stimulating factor, granulocyte/macrophage colony-stimulating factor and ligand for the fms-like tyrosine kinase 3 instruct cell lineage. Sub-populations of HSCs express each of the cytokine receptors that …

0301 basic medicineLineage (genetic)medicine.medical_treatmentReviewBiologyCell and Developmental Biology03 medical and health sciencesimmune cells0302 clinical medicinemedicineMacrophageEpigeneticsCytokine bindinglcsh:QH301-705.5Cell Biologyhematopoiesishematopoietic stem cellsCell biologyHaematopoiesis030104 developmental biologyCytokinelcsh:Biology (General)030220 oncology & carcinogenesisDNA methylationblood cellsStem cellfate determinationDevelopmental BiologyFrontiers in Cell and Developmental Biology
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