Search results for "26"
showing 10 items of 3194 documents
Translating recent results from the Cardiovascular Outcomes Trials into clinical practice: recommendations from the Central and Eastern European Diab…
2017
Aims These recommendations aim to improve care for patients with type 2 diabetes (T2D) at high cardiovascular (CV) risk in Central and Eastern Europe. Cardiovascular disease (CVD) and/or chronic kidney disease (CKD) are major interdependent comorbidities in patients with T2D, accounting for 50% of mortality. Following recent CV outcomes trial (CVOT) results, including those from EMPA-REG OUTCOME®, LEADER®, SUSTAIN™-6 and, most recently, the CANVAS study, it is essential to develop regional expert consensus recommendations to aid physicians in interpreting these newest data to clinical practice. Methods The Central and Eastern European Diabetes Expert Group (CEEDEG) followed a Delphi method …
A One-Step PCR-Based Assay to Evaluate the Efficiency and Precision of Genomic DNA-Editing Tools
2017
Despite rapid progress, many problems and limitations persist and limit the applicability of gene-editing techniques. Making use of meganucleases, TALENs, or CRISPR/Cas9-based tools requires an initial step of pre-screening to determine the efficiency and specificity of the designed tools. This step remains time consuming and material consuming. Here we propose a simple, cheap, reliable, time-saving, and highly sensitive method to evaluate a given gene-editing tool based on its capacity to induce chromosomal translocations when combined with a reference engineered nuclease. In the proposed technique, designated engineered nuclease-induced translocations (ENIT), a plasmid coding for the DNA-…
Qualitative and Quantitative Analysis of Cardiac Progenitor Cells in Cases of Myocarditis and Cardiomyopathy
2018
We aimed to identify and quantify CD117+ and CD90+ endogenous cardiac progenitor cells (CPC) in human healthy and diseased hearts. We hypothesize that these cells perform a locally acting, contributing function in overcoming medical conditions of the heart by endogenous means. Human myocardium biopsies were obtained from 23 patients with the following diagnoses: Dilatative cardiomyopathy (DCM), ischemic cardiomyopathy (ICM), myocarditis, and controls from healthy cardiac patients. High-resolution scanning microscopy of the whole slide enabled a computer-based immunohistochemical quantification of CD117 and CD90. Those signals were evaluated by Definiens Tissue Phenomics® Technology. Co-loca…
Pinpointing the PRDM9-PRDM7 Gene Duplication Event During Primate Divergence
2021
Studies on the function of PRDM9 in model systems and its evolution during vertebrate divergence shed light on the basic molecular mechanisms of hybrid sterility and its evolutionary consequences. However, information regarding PRDM9-homolog, PRDM7, whose origin is placed in the primate evolutionary tree, as well as information about the fast-evolving DNA-binding zinc finger array of strepsirrhine PRDM9 are scarce. Thus, we aimed to narrow down the date of the duplication event leading to the emergence of PRDM7 during primate evolution by comparing the phylogenetic tree reconstructions of representative primate samples of PRDM orthologs and paralogs. To confirm our PRDM7 paralogization patt…
Novel Mutations in the Asparagine Synthetase Gene (ASNS) Associated With Microcephaly
2018
Microcephaly is a devastating condition defined by a small head and small brain compared to the age- and sex-matched population. Mutations in a number of different genes causative for microcephaly have been identified, e.g., MCPH1, WDR62, and ASPM. Recently, mutations in the gene encoding the enzyme asparagine synthetase (ASNS) were associated to microcephaly and so far 24 different mutations in ASNS causing microcephaly have been described. In a family with two affected girls, we identified novel compound heterozygous variants in ASNS (c.1165G > C, p.E389Q and c.601delA, p.M201Wfs∗28). The first mutation (E389Q) is a missense mutation resulting in the replacement of a glutamate residue …
Transcriptional Control of Quality Differences in the Lipid-Based Cuticle Barrier in Drosophila suzukii and Drosophila melanogaster
2020
Cuticle barrier efficiency in insects depends largely on cuticular lipids. To learn about the evolution of cuticle barrier function, we compared the basic properties of the cuticle inward and outward barrier function in adults of the fruit flies Drosophila suzukii and Drosophila melanogaster that live on fruits sharing a similar habitat. At low air humidity, D. suzukii flies desiccate faster than D. melanogaster flies. We observed a general trend indicating that in this respect males are less robust than females in both species. Xenobiotics penetration occurs at lower temperatures in D. suzukii than in D. melanogaster. Likewise, D. suzukii flies are more susceptible to contact insecticides …
Responsiveness to anti-PD-1 and anti-CTLA-4 immune checkpoint blockade in SB28 and GL261 mouse glioma models.
2018
Immune checkpoint blockade (ICB) is currently evaluated in patients with glioblastoma (GBM), based on encouraging clinical data in other cancers, and results from studies with the methylcholanthrene-induced GL261 mouse glioma. In this paper, we describe a novel model faithfully recapitulating some key human GBM characteristics, including low mutational load, a factor reported as a prognostic indicator of ICB response. Consistent with this observation, SB28 is completely resistant to ICB, contrasting with treatment sensitivity of the more highly mutated GL261. Moreover, SB28 shows features of a poorly immunogenic tumor, with low MHC-I expression and modest CD8(+) T-cell infiltration, suggest…
El dantisme de Manuel de Montoliu
2007
In the year 1903 Manuel de Montoliu published the first Catalan version, in prose and poetry, of "La Vida Nova", which meant an important stage within the history of pre-Raphaelitism and modernism in Catalonia. In this article the first Dantesque influences in the poetic production of the translator will be detected, the stage of preparation and of presentation of the text will be analysed and, finally, the article will study the progressions that the exegesis of Dante's work determines in Montoliu's thought. This global vision will leave a redimensioned image of the critic which concerns the originality of his positions, and it will be demonstrated that the interpretati…
Design and Culture of the Territory: Ecomuseo del Grano e del Pane in the Salemi Museum System
2019
The article deals with design experimentation in the complex and composite field of design for the culture of the territory, aimed at enhancing the particular resources common to the Mediterranean areas, through an incremental and collaborative design methodology. The town of Salemi (TP) inherited an extraordinary concentration of cultural heritage from its history; in particular the preparation and display of the decorated breads for the Festivity of San Giuseppe, occurring on March 19th, which expresses a profound religiosity and a complex rituality, shared, handed down and recognized as an intan- gible cultural asset to be protected. In this territorial context, a vision aimed at encour-…
No me banco la gente así. Un estudio comparativo del uso de palabras coloquiales y malsonantes en conversaciones informales en tres comunidades de ha…
2019
The present article aims at describing and comparing the use of slang words and swearwords in teenage talk from three different Spanish speaking communities. The analysis builds on spontaneous teenage conversations from the corpus of teenage language Corpus Oral de Lenguaje Adolescente (COLA). This corpus contains informal conversations from Madrid, Buenos Aires and Santiago de Chile; and for this study one conversation from each city has been selected. The aim of the analysis is twofold, in the first place to describe and compare the slang words and swearwords registered in the three conversations, and secondly to study the pragmatic functions of these words. The analysis of the slang word…