Search results for "3a"
showing 10 items of 351 documents
Evaluation of Cytochrome P450 Activities in Human Hepatocytes In Vitro
2011
Major hepatic cytochrome P450 activities (CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4) can be simultaneously examined in human hepatocytes by incubation with a cocktail of multiple specific probes. Cocktail strategy in combination with mass spectrometry is shown to be a robust, fast, and sensitive procedure for P450 activity assessment. This procedure allows a drastic reduction of the number of cells required in the assay and sample analysis time and increases throughput and reproducibility. Major applications of the probe cocktail strategy are P450 phenotyping of hepatocytes and induction studies.
miR-133a Enhances the Protective Capacity of Cardiac Progenitors Cells after Myocardial Infarction
2014
Summary miR-133a and miR-1 are known as muscle-specific microRNAs that are involved in cardiac development and pathophysiology. We have shown that both miR-1 and miR-133a are early and progressively upregulated during in vitro cardiac differentiation of adult cardiac progenitor cells (CPCs), but only miR-133a expression was enhanced under in vitro oxidative stress. miR-1 was demonstrated to favor differentiation of CPCs, whereas miR-133a overexpression protected CPCs against cell death, targeting, among others, the proapoptotic genes Bim and Bmf. miR-133a-CPCs clearly improved cardiac function in a rat myocardial infarction model by reducing fibrosis and hypertrophy and increasing vasculari…
Cellule staminali di osteosarcoma umano 3AB-OS: un modello per analizzare le proprietà oncogeniche di p53 mutata.
2013
Alterazioni del gene TP53 si riscontrano in circa il 50% dei tumori umani (1). Negli ultimi anni è stato dimostrato che la proteina mutata p53 (mp53) acquisisce nuove proprietà oncogeniche, definite “gain of function” (GOF), che contribuiscono alla progressione tumorale (2). In questo studio è stato analizzato lo stato del gene TP53, della proteina da esso espressa e il loro ruolo nella promozione della proliferazione, invasività, resistenza all’apoptosi e staminalità delle cellule 3AB-OS, una linea tumorale staminale precedentemente isolata dalle cellule di osteosarcoma umano MG63 (3). Analisi comparative di RT-PCR, Methylation-Specific-PCR (MSP), Fluorescent-in situ-hybridization (FISH) e…
Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages.
2013
Formation of cilia, microtubule-based structures that function in propulsion and sensation, requires Kif3a, a subunit of Kinesin II essential for intraflagellar transport (IFT). We have found that, Kif3a is also required to organize centrioles. In the absence of Kif3a, the subdistal appendages of centrioles are disorganized and lack p150(Glued) and Ninein. Consequently, microtubule anchoring, centriole cohesion and basal foot formation are abrogated by loss of Kif3a. Kif3a localizes to the mother centriole and interacts with the Dynactin subunit p150(Glued) . Depletion of p150(Glued) phenocopies the effects of loss of Kif3a, indicating that Kif3a recruitment of p150(Glued) is critical for s…
The retinitis pigmentosa protein RP2 links pericentriolar vesicle transport between the Golgi and the primary cilium.
2010
Photoreceptors are complex ciliated sensory neurons. The basal body and periciliary ridge of photoreceptors function in association with the Golgi complex to regulate the export of proteins from the inner segment to the outer segment sensory axoneme. Here, we show that the retinitis pigmentosa protein RP2, which is a GTPase activating protein (GAP) for Arl3, localizes to the ciliary apparatus, namely the basal body and the associated centriole at the base of the photoreceptor cilium. Targeting to the ciliary base was dependent on N-terminal myristoylation. RP2 also localized to the Golgi and periciliary ridge of photoreceptors, which suggested a role for RP2 in regulating vesicle traffic an…
Primary cilia are required for cerebellar development and Shh-dependent expansion of progenitor pool
2008
Cerebellar granule cell precursors (GCPs), which give rise to the most abundant neuronal type in the mammalian brain, arise from a restricted pool of primary progenitors in the rhombic lip (RL). Sonic hedgehog (Shh) secreted by developing Purkinje cells is essential for the expansion of GCPs and for cerebellar morphogenesis. Recent studies have shown that the primary cilium concentrates components of Shh signaling and that this structure is required for Shh signaling. GCPs have a primary cilium on their surface [Del Cerro, M.P., Snider, R.S. (1972). Studies on the developing cerebellum. II. The ultrastructure of the external granular layer. J Comp Neurol 144, 131-64.]. Here, we show that 1)…
Bimodal tracers synthesis for medical imaging PET/MRI
2015
Today physicians can use a wide variety of medical imaging techniques to establish early and accurate diagnosis. Nevertheless, each modality has its own advantages and drawbacks. This is why bi- or multimodality approach seems interesting. Among them, PET/MRI combination seems very promising because it can bring complementary informations. It is therefore necessary to inject to patients tracers specific to each imaging modality. This work described the synthesis of molecular platforms for MRI and PET imaging, according to 2 different strategies. The first one consisted in the synthesis of a DO3A macrocycle allowing the chelation of both gadolinium for MRI and gallium 68 for PET. The aim her…
Computing with Rational Symmetric Functions and Applications to Invariant Theory and PI-algebras
2012
The research of the first named author was partially supported by INdAM. The research of the second, third, and fourth named authors was partially supported by Grant for Bilateral Scientific Cooperation between Bulgaria and Ukraine. The research of the fifth named author was partially supported by NSF Grant DMS-1016086.
Invariant deformation theory of affine schemes with reductive group action
2015
We develop an invariant deformation theory, in a form accessible to practice, for affine schemes $W$ equipped with an action of a reductive algebraic group $G$. Given the defining equations of a $G$-invariant subscheme $X \subset W$, we device an algorithm to compute the universal deformation of $X$ in terms of generators and relations up to a given order. In many situations, our algorithm even computes an algebraization of the universal deformation. As an application, we determine new families of examples of the invariant Hilbert scheme of Alexeev and Brion, where $G$ is a classical group acting on a classical representation, and describe their singularities.
Auditory cortical and hippocampal-system mismatch responses to duration deviants in urethane-anesthetized rats.
2013
Any change in the invariant aspects of the auditory environment is of potential importance. The human brain preattentively or automatically detects such changes. The mismatch negativity (MMN) of event-related potentials (ERPs) reflects this initial stage of auditory change detection. The origin of MMN is held to be cortical. The hippocampus is associated with a later generated P3a of ERPs reflecting involuntarily attention switches towards auditory changes that are high in magnitude. The evidence for this cortico-hippocampal dichotomy is scarce, however. To shed further light on this issue, auditory cortical and hippocampal-system (CA1, dentate gyrus, subiculum) local-field potentials were …