Search results for "3b"

showing 10 items of 143 documents

The endoperoxide ascaridol shows strong differential cytotoxicity in nucleotide excision repair-deficient cells

2011

Targeting synthetic lethality in DNA repair pathways has become a promising anti-cancer strategy. However little is known about such interactions with regard to the nucleotide excision repair (NER) pathway. Therefore, cell lines with a defect in the NER genes ERCC6 or XPC and their normal counterparts were screened with 53 chemically defined phytochemicals isolated from plants used in traditional Chinese medicine for differential cytotoxic effects. The screening revealed 12 drugs that killed NER-deficient cells more efficiently than proficient cells. Five drugs were further analyzed for IC50 values, effects on cell cycle distribution, and induction of DNA damage. Ascaridol was the most effe…

RAD23BDNA RepairDNA repairDNA damageCyclohexane MonoterpenesBiologyToxicologyCell LineInhibitory Concentration 50HumansCytotoxic T cellMedicine Chinese TraditionalPharmacologyDose-Response Relationship DrugCell cycleAntineoplastic Agents PhytogenicMolecular biologyPeroxidesG2 Phase Cell Cycle CheckpointsCell cultureCancer cellMonoterpenesM Phase Cell Cycle CheckpointsReactive Oxygen SpeciesDNA DamageDrugs Chinese HerbalNucleotide excision repairToxicology and Applied Pharmacology

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The yeast putative transcriptional repressor RGM1 is a proline-rich zinc finger protein.

1991

Abstract I have cloned a yeast gene, RGM1, which encodes a proline-rich zinc, finger protein. rgm1 mutants do not show any obvious phenotype but overexpression of RGM1 gene greatly impairs cell growth. The proline-rich region of RGM1 attached to a heterologous DNA binding domain is able to repress the expression of the target gene. RGM1 shares similar zinc finger motifs with the mammalian Egr (early growth response) proteins as well as proline-rich sequences with a high serine and threonine content, suggesting that RGM1 and Egr proteins could have functional similarities.

Recombinant Fusion ProteinsMolecular Sequence DataRestriction MappingGene ExpressionSaccharomyces cerevisiaeBiologyZIC2TransfectionSequence Homology Nucleic AcidGene expressionGeneticsAmino Acid SequenceCloning MolecularLIM domainSIN3BZinc fingerBase SequenceZinc FingersDNA-binding domainZinc finger nucleaseRING finger domainbody regionsRepressor ProteinsBiochemistryMutagenesisCarbohydrate MetabolismPlasmidsNucleic acids research

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Investigating CRISPR-CAS13b as a tool for the RNA editing of CFTR mRNA with premature stop codon

2020

Background and Rationale Some CF patients are compound heterozygous or homozygous for nonsense mutations in the CFTR gene. Mutant CFTR gene coding for transcripts with premature termination codons (PTCs) is responsible for truncated CFTR protein and for a severe form of the disease. In a precision medicine framework the “REPAIRv2” (RNA Editing for Programmable A to I Replacement v2) tool, developed in the laboratory of Dr. Feng Zhang (USA), seems a good alternative to restore the full-length CFTR protein by editing its mRNA containing PTCs. This new approach is based on the possibility of targeting a deaminase enzyme (huADAR2) to a specific Adenosine, to be edited to Inosine (G analogue), o…

Settore BIO/18 - GeneticamRNA editing CFTR CRISPR-dCAS13b

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Probabilistic squares and hexagons of opposition under coherence

2017

Various semantics for studying the square of opposition and the hexagon of opposition have been proposed recently. We interpret sentences by imprecise (set-valued) probability assessments on a finite sequence of conditional events. We introduce the acceptability of a sentence within coherence-based probability theory. We analyze the relations of the square and of the hexagon in terms of acceptability. Then, we show how to construct probabilistic versions of the square and of the hexagon of opposition by forming suitable tripartitions of the set of all coherent assessments on a finite sequence of conditional events. Finally, as an application, we present new versions of the square and of the…

Settore MAT/06 - Probabilita' E Statistica MatematicaSquare of opposition02 engineering and technologycoherence conditional events hexagon of opposition imprecise probability square of opposition quantified sentences tripartition01 natural sciencesSquare (algebra)Theoretical Computer ScienceSet (abstract data type)Probability theoryArtificial IntelligenceFOS: Mathematics0202 electrical engineering electronic engineering information engineering0101 mathematicsMathematicsApplied MathematicsProbability (math.PR)010102 general mathematicsProbabilistic logicMathematics - LogicCoherence (statistics)Settore MAT/01 - Logica MatematicaImprecise probabilityAlgebra03b48020201 artificial intelligence & image processingLogic (math.LO)AlgorithmMathematics - ProbabilitySoftwareSentence

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CCDC 2053830: Experimental Crystal Structure Determination

2021

Related Article: Jevgeņija Lugiņina, Martin Linden, Māris Bazulis, Viktors Kumpiņš, Anatoly Mishnev, Sergey A. Popov, Tatiana S. Golubeva, Siegfried R. Waldvogel, Elvira E. Shults, Māris Turks|2021|Eur.J.Org.Chem.|2021|2557|doi:10.1002/ejoc.202100293

Space GroupCrystallographyCrystal SystemCrystal Structure(17S)-17-{[3beta-hydroxy-28-norlupane]}nitrile oxideCell ParametersExperimental 3D Coordinates

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CCDC 1047644: Experimental Crystal Structure Determination

2017

Related Article: Michael Mirion, Lars Andernach, Caroline Stobe, Joaquin Barjau, Dieter Schollmeyer, Till Opatz, Arne Lützen, Siegfried R. Waldvogel|2015|Eur.J.Org.Chem.|2015|4876|doi:10.1002/ejoc.201500600