Search results for "5'"
showing 10 items of 358 documents
The interaction of native calf thymus DNA with Zn(II)-N,N'-bis(5,5'-triethylammoniomethyl-salicylidene)phenylendiimine
2006
The interaction between native calf thymus deoxyribonucleic acid (DNA) and Fe-III-N,N'-ethylene-bis (salicylideneiminato)chloride, Fe(Salen)Cl, was investigated in aqueous solutions by UV-visible (UV-vis) absorption, circular clichroisin (CD), thermal denaturation and viscosity measurements. The results obtained from CD, UV-vis and viscosity measurements exclude DNA intercalation and can be interpreted in terms of an electrostatic binding between the Fe(Salen)(+) cation and the phosphate groups of DNA. The trend of the UV-vis absorption band of the Fe(Salen)Cl complex at different ratios [DNA(phosphate)]/[Fe(Salen)Cl] and the large increase of the melting temperature of DNA in the presence …
Etude du rôle du récepteur nucléaire CAR, Constitutive Androstane Receptor, dans le métabolisme des lipides et la susceptibilité à l'athérosclérose
2011
The Constitutive Androstane Receptor (CAR) belongs to the subfamily of nuclear receptors NR1. Initially described as an orphan receptor, CAR is activated by a large number of exogenous molecules and acts as a xenosensor. The activation of CAR by these ligands stimulates transcription of phase I, II and III enzymes required for the detoxification and elimination of xenobiotics. Furthermore CAR is also involved in the metabolism of endogenous molecules such as bile acids, bilirubin or thyroid hormones. CAR has recently been the subject of numerous independent studies that have highlighted his involvement in major metabolic pathways including gluconeogenesis, lipogenesis and lipoprotein metabo…
Efecto de la exposición natural al alérgeno sobre la respuesta máxima a adenosina 5’-monofosfato, concentraciones de óxido nítrico exhalado y pH del …
2014
El efecto de la exposición a los alérgenos en la capacidad de respuesta de la vía aérea y el óxido nítrico exhalado (NO) está bien documentado, pero no se dispone de información sobre los cambios inducidos por alérgenos en la meseta de la respuesta a la adenosina 5'-monofosfato (AMP), en el NO bronquial (J 'aw NO) y alveolar (CA NO), y en el atrapamiento de la vía aérea. Objetivos: Determinar el efecto de la exposición alergénica natural en la forma de la curva de concentración-respuesta a AMP, producción de NO en las vías respiratorias bronquiales y alveolares, concentración del pH del condensado del aire exhalado, y atrapamiento de la vía aérea inducido por metacolina y AMP. Métodos: Hipe…
Chromatin structure of the 5′ flanking region of the yeastLEU2 gene
1989
The chromatin structure of theLEU2 gene and its flanks has been studied by means of nuclease digestion, both with micrococcal nuclease and DNase I. The gene is organized in an array of positioned nucleosomes. Within the promoter region, the nucleosome positioning places the regulatory sequences, putative TATA box and upstream activator sequence outside the nucleosomal cores. The tRNA3 Leu gene possesses a characteristic structure and is protected against nucleases. Most of the 5′ flank is sensitive to DNase I digestion, although no clear hypersensitive sites were found. The chromatin structure is independent of either the transcriptional state of the gene or the chromosomal or episomal loca…
Induction of (2′−5′)oligoadenylate synthetase in the marine spongesSuberites domunculaandGeodia cydoniumby the bacterial endotoxin lipopolysaccharide
2002
Recent studies have shown that the Porifera, with the examples of the demosponges Suberites domuncula and Geodia cydonium, comprise a series of pathways found also in the immune system of Deuterostomia, such as vertebrates, but are absent in Protostomia, with insects or nematodes as examples. One pathway is the (2'-5')oligoadenylate synthetase [(2-5)A synthetase] system. In the present study we show that crude extracts from tissue of S. domuncula collected from the sea display a considerable amount of (2-5)A synthetase activity; 16% of the ATP substrate is converted to the (2-5)A product, while tissue from specimens which were kept for 6 months in an aquarium shows only 1% of conversion. As…
Modulation of Nuclear Matrix-associated 2′,5′-Oligoadenylate Metabolism and Ribonuclease L Activity in H9 Cells by Human Immunodeficiency Virus
1989
Human T cells (H9), infected with the HTLV-IIIB strain of the human immunodeficiency virus (HIV-1), have been used to study the alteration of 2',5'-oligoadenylate [2'-5')A) metabolism in relation to virus production. The synthesis of (2'-5')A was determined to proceed in close association with the nuclear matrix. After HIV infection the (2'-5')A synthetase activity increased from 1.1 to 1.5 pmol of (2'-5')A synthesized/100 micrograms of nuclear matrix protein (during a 3-h in vitro incubation period) to 8.2 pmol at day 3 after infection. Then the activity dropped to the initial values. In non-infected H9 cells the (2'-5')A synthetase activity remained unchanged. Simultaneously with the decr…
CCDC 672474: Experimental Crystal Structure Determination
2008
Related Article: Hyui Sul Lee, S.-O.Hauber, D.Vindus, M.Niemeyer|2008|Inorg.Chem.|47|4401|doi:10.1021/ic800029z
CCDC 1477302: Experimental Crystal Structure Determination
2016
Related Article: C. Köhler, E. Rentschler|2016|Dalton Trans.|45|12854|doi:10.1039/C6DT02023B
CCDC 2047570: Experimental Crystal Structure Determination
2021
Related Article: Eugenia Peresypkina, Kevin Grill, Barbara Hiltl, Alexander V. Virovets, Werner Kremer, Jan Hilgert, Wolfgang Tremel and Manfred Scheer|2021|Angew.Chem.,Int.Ed.|60|12132|doi:10.1002/anie.202103178
CCDC 2047569: Experimental Crystal Structure Determination
2021
Related Article: Eugenia Peresypkina, Kevin Grill, Barbara Hiltl, Alexander V. Virovets, Werner Kremer, Jan Hilgert, Wolfgang Tremel and Manfred Scheer|2021|Angew.Chem.,Int.Ed.|60|12132|doi:10.1002/anie.202103178