Search results for "573"
showing 8 items of 128 documents
The difficulty of applying exclusion criteria in ethical portfolios
2018
A main problem for socially responsible investors is deciding which companies should be included in their investment portfolio. Small investors may decide not to invest in companies they know are not behaving responsibly. Therefore, discredited companies will not be included in their portfolios, as they will understand those companies cannot be defined as ethical. To implement this procedure properly and apply it to big portfolios is a complex exercise which requires big amounts of information and the problems faced by investors applying such exclusion criteria to select their portfolio.
Physical exercise induces rapid release of small extracellular vesicles into the circulation
2015
Cells secrete extracellular vesicles (EVs) by default and in response to diverse stimuli for the purpose of cell communication and tissue homeostasis. EVs are present in all body fluids including peripheral blood, and their appearance correlates with specific physiological and pathological conditions. Here, we show that physical activity is associated with the release of nano-sized EVs into the circulation. Healthy individuals were subjected to an incremental exercise protocol of cycling or running until exhaustion, and EVs were isolated from blood plasma samples taken before, immediately after and 90 min after exercise. Small EVs with the size of 100–130 nm, that carried proteins character…
A One-Step PCR-Based Assay to Evaluate the Efficiency and Precision of Genomic DNA-Editing Tools
2017
Despite rapid progress, many problems and limitations persist and limit the applicability of gene-editing techniques. Making use of meganucleases, TALENs, or CRISPR/Cas9-based tools requires an initial step of pre-screening to determine the efficiency and specificity of the designed tools. This step remains time consuming and material consuming. Here we propose a simple, cheap, reliable, time-saving, and highly sensitive method to evaluate a given gene-editing tool based on its capacity to induce chromosomal translocations when combined with a reference engineered nuclease. In the proposed technique, designated engineered nuclease-induced translocations (ENIT), a plasmid coding for the DNA-…
The cell nucleus. A study in Burgundy
2019
ABSTRACT Wilhelm Bernhard’s revolutionary microscopy techniques helped him put forward the hypothesis of specialized compartmentalization of the nucleus. He also described for the first time the nuclear bodies and peri-chromatin fibrils, and demonstrated that these granules contain an RNA component. The tradition of biennial workshops, named after this great scientist, continues, and this year it took place in the heart of Burgundy, in Dijon, France (May 20–24, 2019, organized by INSERM UMR1231, UBFC), where well-fed participants emphasized the importance of viewing the cell nucleus as a hub of specialized colloidal compartments that orchestrate replication, transcription and nuclear transp…
Aplicación de las técnicas FISH, PCR específica y 16S-ARDRA al estudio de la población bacteriana asociada al proceso de vinificación
2009
El vino es el producto de la fermentación del mosto de uva y en esta transformación intervienen gran cantidad de microorganismos. Estos microorganismos pueden ser beneficiosos, como S. cerevisiae y O. oeni, o perjudiciales como la gran mayoría de las bacterias lácticas (BL) y todas las bacterias acéticas (BA). La adopción de medidas de control tempranas que eviten las alteraciones microbianas durante el proceso de vinificación o crianza es necesaria para obtener un vino de calidad. Por ello, el objetivo principal de este trabajo ha sido el desarrollo de una metodología rápida que permita detectar, identificar y cuantificar las BL y acéticas en el vino.Para ello se desarrollaron de sondas fl…
Estudio y caracterización molecular de la producción de aminas biógenas por parte de bacterias lácticas de origen enológico.
2005
Dada la influencia negativa de la presencia de aminas biógenas en el vino, por el efecto perjudicial sobre la salud humana y por la disminución de la calidad del vino y dada también la presencia de las bacterias lácticas (BL) en los mismos, en esta tesis nos planteamos determinar la importancia de las BL en la presencia de estas aminas en el vino, así como que factores podían influir en la concentración de las mismas o en la mayor o menor producción por parte de las BL.En primer lugar, desarrollamos un método enzimático que permite analizar la concentración de histamina en vino y la producción de la misma por BL, a continuación analizamos la concentración de histamina, tiramina, feniletilam…
L'éthique appliquée à la communication professionnelle
2018
Ce numéro propose une exploration des enjeux éthiques tels qu'ils sont présents aujourd'hui dans différents domaines professionnels du secteur de la communication. Dans l'opinion publique actuelle ces deux termes (« communication », comme métier et activité professionnelle, et « éthique ») semblent parfois opposés : nous rappelions déjà dans le numéro 5 quelques données concernant la méfiance que la communication professionnelle inspire, à l'époque des spin doctors et, plus récemment, des fake news et des faits alternatifs. En réalité, l'éthique est un enjeu de toujours et de toutes les sphères de la vie humaine. C'est inévitable donc que la communication professionnelle soit traversée de f…