Search results for "93"
showing 10 items of 1707 documents
Holocene climate variability in north-eastern Italy: potential influence of the NAO and solar activity recorded by speleothem data
2012
Abstract. Here we present high-resolution stable isotope and lamina thickness profiles as well as radiocarbon data for the Holocene stalagmite ER 76 from Grotta di Ernesto (north-eastern Italy), which was dated by combined U-series dating and lamina counting. ER 76 grew between 8 ka (thousands of years before 2000 AD) and today, with a hiatus from 2.6 to 0.4 ka. Data from nine meteorological stations in Trentino show a significant influence of the North Atlantic Oscillation (NAO) on winter temperature and precipitation in the cave region. Spectral analysis of the stable isotope signals of ER 76 reveals significant peaks at periods of 110, 60–70, 40–50, 32–37 and around 25 a. Except for the …
Steroids aid in human decomposition fluid identification in soils of temporary mass graves from World War II
2020
Abstract Steroids are widely used for the detection of faecal matter and also – in recent years – for characterising human decomposition in the terrestrial environment. Until now it was not clear whether all commonly used faecal (i.e. 5β-stanols, 5β-stanones and bile acids) and tissue derived steroids (i.e. cholesterol, 5α-cholestanol and 5α-cholestanone) could reveal the presence of human decomposition products in temporary graves. In this study, soil was sampled from three putative mass graves where concentration camp prisoners were temporarily buried for 10 months at the end of World War II (1944 – 45). We hypothesised that soil from the putative temporary mass graves exhibits elevated c…
Climate indices in historical climate reconstructions: a global state of the art
2021
Narrative evidence contained within historical documents and inscriptions provides an important record of climate variability for periods prior to the onset of systematic meteorological data collection. A common approach used by historical climatologists to convert such qualitative information into continuous quantitative proxy data is through the generation of ordinal-scale climate indices. There is, however, considerable variability in the types of phenomena reconstructed using an index approach and the practice of index development in different parts of the world. This review, written by members of the PAGES (Past Global Changes) CRIAS working group – a collective of climate historians a…
Coarsely crystalline cryogenic cave carbonate – a new archive to estimate the Last Glacial minimum permafrost depth in Central Europe
2012
Abstract. Cryogenic cave carbonate (CCC) represents a specific type of speleothem whose precipitation is triggered by freezing of mineralized karst water. Coarsely crystalline CCC, which formed during slow freezing of water in cave pools, has been reported from 20 Central European caves located in Germany, the Czech Republic, Slovakia and Poland. All these caves are situated in an area which was glacier-free during the Weichselian. Whereas the formation of usual types of speleothems in caves of this region usually ceased during the glacials, coarsely crystalline CCC precipitation was restricted to glacial periods. Since this carbonate type represents a novel, useful paleoclimate proxy, data…
Role of Bacillus thuringiensis Cry1A toxins domains in the binding to the ABCC2 receptor from Spodoptera exigua
2018
Abstract Cry proteins from Bacillus thuringiensis (Bt) have been used to control insect pests either as formulated sprays or as in Bt-crops. However, field-evolved resistance to Bt proteins is threatening the long-term use of Bt products. The SeABCC2 locus has been genetically linked to resistance to a Bt bioinsecticide (Xentari™) in Spodoptera exigua (a mutation producing a truncated form of the transporter lacking an ATP binding domain was found in the resistant insects). Here, we investigated the role of SeABCC2 in the mode of action of Cry1Aa, Cry1Ab, Cry1Ac, Cry1Ca, and two Cry1A-1Ca hybrids by expressing the receptor in Sf21 and HEK293T cell lines. Cell toxicity assays showed that Sf2…
Enhancing heterologous protein expression and secretion in HEK293 cells by means of combination of CMV promoter and IFNα2 signal peptide
2016
Efficient production and secretion of recombinant proteins in mammalian cell lines relies in a combination of genetic, metabolic and culture strategy factors. The present work assesses the influence of two key genetic components of expression vectors (promoter and signal peptide) on protein production and secretion effciency in HEK293 cells expressing eGFP as a reporter protein. Firstly, the strength of 3 different promoters was evaluated using transient expression methods. Flow cytometry analysis revealed that the highest level of intracellular protein expression was found when eGFP was under the control of CMV promoter, being 3-times higher in comparison to the rest of the promoters teste…
Effect of pulp cell number and assimilate availability on dry matter accumulation rate in a banana fruit (Musa sp. AAA group 'Grande Naine' (Cavendis…
2001
Fruit position on the bunch (inflorescence) is an important part of variability in banana fruit weight at harvest, as fruits at the bottom of the bunch (distal fruits) are approx. 40% smaller than those at the top (proximal fruits). In this study, the respective roles of cell number and cell filling rate in the development of pulp dry weight are estimated. To this end, the source/sink ratio in the plant was altered at different stages of fruit development. Leaf shading (reducing resource availability), bunch bagging (increasing sink activity by increasing fruit temperature), and bunch trimming (decreasing sink size by fruit pruning), applied once cell division had finished, showed that the …
New national and regional Annex I Habitat records: from #21 to #25
2021
New Italian data on the distribution of the Annex I Habitats 3170*, 6110*, 91E0*, 9320, 9330 are reported in this contribution. Specifically, one new occurrence in Natura 2000 sites is presented and six new cells are added in the European Environment Agency 10 km × 10 km reference grid. The new data refer to the Italian administrative regions of Sardinia, Sicily and Umbria.
Incorporating Biotic Information in Species Distribution Models: A Coregionalized Approach
2021
In this work, we discuss the use of a methodological approach for modelling spatial relationships among species by means of a Bayesian spatial coregionalized model. Inference and prediction is performed using the integrated nested Laplace approximation methodology to reduce the computational burden. We illustrate the performance of the coregionalized model in species interaction scenarios using both simulated and real data. The simulation demonstrates the better predictive performance of the coregionalized model with respect to the univariate models. The case study focus on the spatial distribution of a prey species, the European anchovy (Engraulis encrasicolus), and one of its predator spe…
New national and regional Annex I Habitat records: from #13 to #15
2020
New data on the distribution of the Annex I Habitats 3160, 7210* and 9320 are reported in this contribution. In detail, 24 new occurrences in Natura 2000 Sites are presented and 42 new cells in the EEA 10 km x 10 km Reference grid are added. The new data refer to Italy and in particular to the Administrative Regions Lombardy, Sardinia, and Sicily.