Search results for "A2"
showing 10 items of 1101 documents
Esclusioni etniche nei regolamenti cultuali greci: la norma di Paros (IG XII 5, 225)
2019
This study aims to examine a well-known inscription (IG XII.5, 225) dated to the mid-fifth century BC and found in Paroikia, near the acropolis of the ancient polis of Paros. It shows a typical formula of access limitation to local cults: according to most scholars, the cult in question is to be identified with the worship of Kore Astos (the Citizen), who is mentioned in the second line. The Dorians are excluded from this cult, as is shown by the expression (xenoi Dorie) appearing at the beginning of the text in a very relevant position. On the basis of a close comparison between this text and other epigraphs as well as literary documents containing proscriptions which prevent foreigners fr…
Images of the imagined city
2018
The essay investigates one of the projects in the competition for the expansion of the North area of Palermo named ZEN 2 and designed by the architects Pasquale Culotta, Giuseppe Laudicina, Teresa Cannarozzo, Anna Maria Fundarò and Tilde Marra, in 1970. The essay aims at illustrating the spatial, figurative and urban qualities of it. The analysis of the project has been guided by a written text and the graphic support, that is the project report and the attached drawings, in Indian Ink, made by the architects themselves, indeed very often fragmented and difficult to be interpreted. In this regard, the support of digital drawing in two-dimensional and three-dimensional form, based on the cre…
Virtual tour through the towers of the defensive system in the province of Salerno
2019
The coastal towers of the defensive system in the province of Salerno are architectures developed over time; they are buildings belonging to an extended strategic plan of the coastal territory useful to defend populations and cities from the ancient enemy attacks. Hence, their location comes from careful studies in which the orographic characteristics of the place and the visual connection between a tower and another played a fundamental role.The aim of this work is to focus the attention on the visual communication between the towers, or on the “dialogue” that the different artefacts entertain each other. In order to pursue this objective, a virtual tour that connects the different ancient…
Characterization of Acylating and Deacylating Activities of an Extracellular Phospholipase A2 in a Water-Restricted Environment
1994
The behavior of porcine pancreatic phospholipase A2 (ppPLA2) in monophasic low-water media has been explored, for the first time, in a systematic manner. It has been investigated how a number of variables can modulate both acylating and deacylating activities of the enzyme, and several interesting, unexpected results are presented. Among the most relevant, when placing ppPLA2 in the water-restricted environment, are the following: (i) it displays a remarkable alteration of its specificity toward the substrate polar head relative to all-water medium; (ii) it is quite severely inhibited by lysophosphatidylcholine (LPC), which has important implications, particularly concerning its acylation a…
Impact of CMV and EBV seropositivity on CD8 T lymphocytes in an old population from West-Sicily.
2007
Abstract Herpes viruses (particularly CMV and to some extent EBV) might play a role in accelerating the deterioration of immune functions with age. Indeed, it has been demonstrated that chronic infection with CMV causes an expansion of specific CD8 T lymphocytes and that this is related to a shrinkage of the T cell repertoire in very elderly people, predicting mortality. We have analysed CD8 T cells in young and old healthy Sicilians who were both CMV- and EBV-seropositive. Our data confirm expansions of T cells specific for the HLA-A2-restricted pp65 (495–503) CMV epitope up to nearly 14% of total peripheral CD8 cells in certain elderly individuals (range 0–14%). However, the mean percenta…
Identification of a Conserved HLA-A2-Restricted Decapeptide from the IE1 Protein (pUL123) of Human Cytomegalovirus
2002
Abstract Control of human cytomegalovirus (HCMV) infection is predominantly mediated by cytolytic CD8 + T lymphocytes (CTL). Among the roughly 200 HCMV-encoded polypeptides, the tegument protein pp65 (ppUL83) and the nonstructural IE1 protein are considered to be dominant CTL targets. Yet the importance of CTL against IE1 for protective immunity against HCMV reactivation and disease has remained elusive. Analyses have been difficult, as all MHC class I presented peptides of IE1 defined so far are located in parts of the protein that are variable between viral strains. In this study a conserved decameric peptide from IE1 (P6, IE1 354–363 ) that bound to HLA-A2 was identified. Using peptide-p…
Evaluating Human T-Cell Therapy of Cytomegalovirus Organ Disease in HLA-Transgenic Mice
2015
Reactivation of human cytomegalovirus (HCMV) can cause severe disease in recipients of hematopoietic stem cell transplantation. Although preclinical research in murine models as well as clinical trials have provided 'proof of concept' for infection control by pre-emptive CD8 T-cell immunotherapy, there exists no predictive model to experimentally evaluate parameters that determine antiviral efficacy of human T cells in terms of virus control in functional organs, prevention of organ disease, and host survival benefit. We here introduce a novel mouse model for testing HCMV epitope-specific human T cells. The HCMV UL83/pp65-derived NLV-peptide was presented by transgenic HLA-A2.1 in the conte…
Immune evasion proteins gpUS2 and gpUS11 of human cytomegalovirus incompletely protect infected cells from CD8 T cell recognition
2009
AbstractHuman cytomegalovirus (HCMV) encodes four glycoproteins, termed gpUS2, gpUS3, gpUS6 and gpUS11 that interfere with MHC class I biosynthesis and antigen presentation. Despite gpUS2–11 expression, however, HCMV infection is efficiently controlled by cytolytic CD8 T lymphocytes (CTL). To address the role of gpUS2 and gpUS11 in antigen presentation during viral infection, HCMV mutants were generated that expressed either gpUS2 or gpUS11 alone without coexpression of the three other proteins. Fibroblasts infected with these viruses showed reduced HLA-A2 and HLA-B7 surface expression. Surprisingly, however, CTL directed against the tegument protein pp65 and the regulatory IE1 protein stil…
Reversible Supracolloidal Self-Assembly of Cobalt Nanoparticles to Hollow Capsids and Their Superstructures
2017
| openaire: EC/FP7/291364/EU//MIMEFUN The synthesis and spontaneous, reversible supracolloidal hydrogen bond-driven self-assembly of cobalt nanoparticles (CoNPs) into hollow shell-like capsids and their directed assembly to higher order superstructures is presented. CoNPs and capsids form in one step upon mixing dicobalt octacarbonyl (Co2CO8) and p-aminobenzoic acid (pABA) in 1,2-dichlorobenzene using heating-up synthesis without additional catalysts or stabilizers. This leads to pABA capped CoNPs (core ca. 5nm) with a narrow size distribution. They spontaneously assemble into tunable spherical capsids (d≈50-200nm) with a few-layered shells, as driven by inter-nanoparticle hydrogen bonds th…
Hydrogen and methane production in extreme thermophilic conditions in two-stage (upflow anaerobic sludge bed) UASB reactor system
2013
Abstract Two-stage hydrogen and methane production in extreme thermophilic (70 °C) conditions was demonstrated for the first time in UASB-reactor system. Inoculum used in hydrogen and methane reactors was granular sludge from mesophilic internal circulation reactor and was first acclimated for extreme thermophilic conditions. In hydrogen reactor, operated with hydraulic retention time (HRT) of 5 h and organic loading rate (OLR) of 25.1 kg COD/m 3 /d, hydrogen yield was 0.73 mol/mol glucose added . Methane was produced in second stage from hydrogen reactor effluent. In methane reactor operated with HRT of 13 h and OLR of 7.8 kg COD/m 3 /d, methane yield was 117.5 ml/g COD added . These resul…