Search results for "ADAM"

showing 10 items of 372 documents

LC–MS Based Cleavage Site Profiling of the Proteases ADAM10 and ADAM17 Using Proteome-Derived Peptide Libraries

2014

A Disintegrin and Metalloproteinase 10 (ADAM10) and ADAM17 catalyze ectodomain shedding of a number of cell surface proteins important for embryonic development and tissue homeostasis. Changes in the expression levels or dysregulated proteolytic activity of ADAM10 and ADAM17 have been shown to play important roles in multiple diseases such as inflammation, cancer, and neurodegenerative disorders. Despite the well documented substrate repertoire of ADAM10 and ADAM17, little is known about their cleavage site specificity. We optimized Q-PICS (Quantitative Proteomics for the Identification of Cleavage Sites) to elucidate the cleavage site specificity of recombinant murine ADAM10 and ADAM17. Tw…

ProteomicsProteasesProteomeQuantitative proteomicsADAM17 ProteinBiologyCleavage (embryo)BiochemistryMass SpectrometryADAM10 ProteinMicePeptide LibraryAnimalsHumansADAM17 ProteinPeptide libraryTissue homeostasisMembrane ProteinsGeneral ChemistryPeptide FragmentsADAM ProteinsBiochemistryEctodomainProteomeAmyloid Precursor Protein SecretasesChromatography LiquidJournal of Proteome Research
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The substrate degradome of meprin metalloproteases reveals an unexpected proteolytic link between meprin β and ADAM10

2012

The in vivo roles of meprin metalloproteases in pathophysiological conditions remain elusive. Substrates define protease roles. Therefore, to identify natural substrates for human meprin α and β we employed TAILS (terminal amine isotopic labeling of substrates), a proteomics approach that enriches for N-terminal peptides of proteins and cleavage fragments. Of the 151 new extracellular substrates we identified, it was notable that ADAM10 (a disintegrin and metalloprotease domain-containing protein 10)—the constitutive α-secretase—is activated by meprin β through cleavage of the propeptide. To validate this cleavage event, we expressed recombinant proADAM10 and after preincubation with meprin…

Proteomicsalpha-2-HS-Glycoproteinmedicine.medical_treatmentADAM10ADAM10 ProteinMice0302 clinical medicine610 Medicine & healthMice KnockoutExtracellular Matrix Proteins0303 health sciencesMetalloproteinaseDegradomeMetalloendopeptidasesMeprinADAM10Terminal amine isotopic labeling of substratesADAM ProteinsElafinBiochemistryTAILSCytokinesMolecular MedicineElafinResearch Article610 Medicine & healthBiologyCell Line03 medical and health sciencesCellular and Molecular NeurosciencemedicineDisintegrinAnimalsHumansAmino Acid SequenceCystatin CMolecular Biology030304 developmental biologyPharmacologyProteaseMeprin; ADAM10; Metalloproteases; Proteomics; TAILS; DegradomeMembrane ProteinsCell BiologyADAM ProteinsHEK293 CellsMembrane proteinbiology.proteinMetalloproteases570 Life sciences; biologyAmyloid Precursor Protein SecretasesCaco-2 Cells030217 neurology & neurosurgery
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P2‐307: A stable G‐quadruplex within the ADAM10 5'‐UTR is involved in translational repression of ADAM10

2011

Psychiatry and Mental healthCellular and Molecular NeuroscienceDevelopmental NeuroscienceFive prime untranslated regionEpidemiologyTranslational repressionChemistryHealth PolicyADAM10Neurology (clinical)Geriatrics and GerontologyG-quadruplexCell biologyAlzheimer's & Dementia
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On symplectically rigid local systems of rank four and Calabi–Yau operators

2013

AbstractWe classify all Sp4(C)-rigid, quasi-unipotent local systems and show that all of them have geometric origin. Furthermore, we investigate which of those having a maximal unipotent element are induced by fourth order Calabi–Yau operators. Via this approach, we reconstruct all known Calabi–Yau operators inducing an Sp4(C)-rigid monodromy tuple and obtain closed formulae for special solutions of them.

Pure mathematicsAlgebra and Number TheoryHadamard productRank (linear algebra)Geometric originUnipotentOperator theoryConvolutionConvolutionAlgebraComputational MathematicsMathematics::Algebraic GeometryMonodromyRigidityCalabi–Yau operatorsCalabi–Yau manifoldHadamard productMathematics::Differential GeometryTupleMathematics::Symplectic GeometryMathematicsJournal of Symbolic Computation
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Hadamard-type theorems for hypersurfaces in hyperbolic spaces

2006

Abstract We prove that a bounded, complete hypersurface in hyperbolic space with normal curvatures greater than −1 is diffeomorphic to a sphere. The completeness condition is relaxed when the normal curvatures are bounded away from −1. The diffeomorphism is constructed via the Gauss map of some parallel hypersurface. We also give bounds for the total curvature of this parallel hypersurface.

Pure mathematicsGauss mapMathematics::Dynamical SystemsMathematics::Complex VariablesHyperbolic spaceSecond fundamental formMathematical analysisCauchy–Hadamard theoremGauss–Kronecker curvatureSecond fundamental formHypersurfaceMathematics::Algebraic GeometryComputational Theory and MathematicsBounded functionHadamard theoremTotal curvatureDiffeomorphismGeometry and TopologyMathematics::Differential GeometryAnalysisConvex hypersurfaceMathematicsDifferential Geometry and its Applications
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Some fourth order CY-type operators with non symplectically rigid monodromy

2012

We study tuples of matrices with rigidity index two in $\Sp_4(\mathbb{C})$, which are potentially induced by differential operators of Calabi-Yau type. The constructions of those monodromy tuples via algebraic operations and middle convolutions and the related constructions on the level differential operators lead to previously known and new examples.

Pure mathematicsGeneral Mathematics010102 general mathematics010103 numerical & computational mathematicsDifferential operator01 natural sciencesMathematics - Algebraic GeometryFourth orderMathematics::Algebraic GeometryMonodromyMathematics - Classical Analysis and ODEsAlgebraic operationClassical Analysis and ODEs (math.CA)FOS: MathematicsHadamard product0101 mathematicsTupleMathematics::Symplectic GeometryAlgebraic Geometry (math.AG)Mathematics
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Improved Bounds for Hermite–Hadamard Inequalities in Higher Dimensions

2019

Let $\Omega \subset \mathbb{R}^n$ be a convex domain and let $f:\Omega \rightarrow \mathbb{R}$ be a positive, subharmonic function (i.e. $\Delta f \geq 0$). Then $$ \frac{1}{|\Omega|} \int_{\Omega}{f dx} \leq \frac{c_n}{ |\partial \Omega| } \int_{\partial \Omega}{ f d\sigma},$$ where $c_n \leq 2n^{3/2}$. This inequality was previously only known for convex functions with a much larger constant. We also show that the optimal constant satisfies $c_n \geq n-1$. As a byproduct, we establish a sharp geometric inequality for two convex domains where one contains the other $ \Omega_2 \subset \Omega_1 \subset \mathbb{R}^n$: $$ \frac{|\partial \Omega_1|}{|\Omega_1|} \frac{| \Omega_2|}{|\partial \Ome…

Pure mathematicsInequalitymedia_common.quotation_subject01 natural sciencesConvexitysymbols.namesakeMathematics - Metric GeometrySettore MAT/05 - Analisi MatematicaHadamard transformHermite–Hadamard inequality0103 physical sciencesClassical Analysis and ODEs (math.CA)FOS: Mathematics[MATH.MATH-AP]Mathematics [math]/Analysis of PDEs [math.AP]Hermite-Hadamard inequality subharmonic functions convexity.0101 mathematicsComputingMilieux_MISCELLANEOUSsubharmonic functionsmedia_commonMathematicsSubharmonic functionHermite polynomialsconvexity010102 general mathematicsMetric Geometry (math.MG)Functional Analysis (math.FA)Mathematics - Functional AnalysisMSC : 26B25 28A75 31A05 31B05 35B50Mathematics::LogicHermite-Hadamard inequalityDifferential geometryMathematics - Classical Analysis and ODEsFourier analysissymbols010307 mathematical physicsGeometry and TopologyThe Journal of Geometric Analysis
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The hermeneutic self-overcoming of culture in interculturalism. Toward an intercultural reading of Hans-Georg Gadamer’s philosophical hermeneutics

2017

RESUMEN El objetivo es analizar la hermenéutica filosófica de Hans-Georg Gadamer con vistas a considerar la posibilidad de llevar a cabo una lectura intercultural a partir de sus planteamientos. Se cuestiona la lectura intercultural que de la hermenéutica de Gadamer lleva a cabo Ram Adhar Mall. Con Gadamer, pero más allá de él, se detectan las posibilidades y limitaciones de su concepto humanista de cultura (Bildung) y se ensaya un modo de autosuperación hermenéutica de la cultura en la interculturalidad. ABSTRACT The objective of the article is to analyze the philosophical hermeneutics of HansGeorg Gadamer in order to explore the possibility of an intercultural reading based on his proposa…

R. A. MallhermeneuticsinterculturalismhermenéuticainterculturalidadH.-G. Gadamer
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La fundamentación de una antropología hermenéutica en 'El nacimiento de la tragedia' de F. Nietzsche

1984

Lectura de 'El nacimiento de la tragedia' de F. Nietzsche como fundamentación de una antropología hermenéutica, demostrando que los diferentes componentes de esta según Gadamer se cumplen punto por punto en el diálogo que en la citada obra Nietzsche sostiene con su maestro de juventud, el compositor y ensayista R. Wagner.

R. WagnerH. G. GadamerUNESCO::FILOSOFÍA:FILOSOFÍA [UNESCO]Antropología filosóficaAntropología hermenéuticaF. Nietzsche
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Ricerca Unipa su diradamento e tasso di crescita dei frutti in piante di nespolo del Giappone

2022

Il trattamento di diradamento dei frutti di nespolo del Giappone, effettuato in una sola occasione, quando i frutti si trovano a circa il 30% delle loro dimensioni finali, ha mostrato alcuni effetti positivi: in primis, i frutti ottenuti in seguito a diradamento hanno una maggiore probabilità di ricadere nelle classi commerciali superiori, le quali sono in genere più apprezzate dai consumatori e pagate a un prezzo più alto al produttore; gli stessi frutti raggiungono al contempo anche un grado zuccherino più alto, altro indice del possibile gradimento del consumatore finale e della richiesta da parte del mercato. Questo lavoro preliminare apre le porte a nuovi studi volti alla valutazione d…

RGBSettore AGR/03 - Arboricoltura Generale E Coltivazioni ArboreematurazionediradamentoNespolo del Giapponequalità
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