Search results for "AMT"

Chromatographic determination of diuretics in urine samples using hybrid micellar mobile phases with fluorimetric detection

1998

Abstract A rapid and simple chromatographic procedure, that uses hybrid micellar mobile phases of sodium dodecyl sulphate (SDS), propanol and phosphate buffer at pH 3, and fluorimetric detection ( λ exc =270 nm, λ em =430 nm), is reported for the determination of mixtures of seven diuretics of diverse efficacy (amiloride, bendroflumethiazide, bumetanide, furosemide, hydroflumethiazide, piretanide and triamterene), in urine samples. The application of a sequential optimization procedure for the resolution of the mixtures of the diuretics was problematic. However, their separation was achieved through the use of an interpretive optimization procedure based on the accurate description of the r…

Atypical presentations of thrombotic thrombocytopenic purpura in middle-aged women with recurrent cerebral macrovascular thrombosis: a case report

2015

Dear Editor, In the current clinical practice, minimal criteria to define thrombotic thrombocytopenic purpura (TTP) are the presence of signs of microangiopathic haemolytic anaemia and low platelet (PLT) count [1]. TTP relapses (20–50 % of cases) are defined as the recurrence of acute TTP symptoms 30 days after the first episode, while exacerbations occur within 30 days [2]. We here report on an atypical case of acquired TTP where minimal criteria were met only after many recurrent macrovascular ischemic events. A 42-year old Caucasian woman with a history of coronary and cerebral ischemic events was admitted on June 2013, following a recurrent transient ischemic attack (TIA). She had sever…

Curcumin downregulates expression of opioid-related nociceptin receptor gene (OPRL1) in isolated neuroglia cells.

2018

Abstract Background: Curcumin (CC) exerts polyvalent pharmacological actions and multi-target effects, including pain relief and anti-nociceptive activity. In combination with Boswellia serrata extract (BS), curcumin shows greater efficacy in knee osteoarthritis management, presumably due to synergistic interaction of the ingredients. Aim: To elucidate the molecular mechanisms underlying the analgesic activity of curcumin and its synergistic interaction with BS. Methods: We performed gene expression profiling by transcriptome-wide mRNA sequencing in human T98G neuroglia cells treated with CC (Curamed), BS, and the combination of CC and BS (CC-BS; Curamin), followed by interactive pathways a…

DEVELOPMENT OF A NEW SATURATED VAPOUR-ANODE, SATURATED VAPOUR-CATHODE AMTEC-LIKE DEVICE

A critical evaluation of caplacizumab for the treatment of acquired thrombotic thrombocytopenic purpura

2020

Introduction: Acquired thrombotic thrombocytopenic purpura (aTTP) is a thrombotic microangiopathy caused by inhibitory autoantibodies against ADAMTS13 protein. Until recently, the combination of plasma exchange (PEX) and immunosuppression has been the standard front-line treatment in this disorder. However, aTTP-related mortality, refractoriness, and relapse are still a matter of concern. Areas covered: The better understanding of the pathophysiological mechanisms of aTTP has allowed substantial improvements in the diagnosis and treatment of this disease. Recently, the novel anti-VWF nanobody caplacizumab has been approved for acute episodes of aTTP. Caplacizumab is capable to block the adh…

Algoritmo diagnóstico-terapéutico de las microangiopatías trombóticas. A propósito de 2 casos

2022

Thrombotic microangiopathies (TMA) are a group of clinical syndromes associated with haemolytic anaemia, thrombocytopenia and organ dysfunction, mainly renal or neurological. They are associated with significant morbidity and mortality, so early diagnosis and treatment are essential. In this article we report two cases of TMA; a patient with thrombotic thrombocytopenic purpura (TTP) and a patient with atypical haemolytic uraemic syndrome (aHUS).

Trematodes Alaria alata raksturojums dažādās infrapopulācijās Latvijā un ģints analīze, pielietojot molekulārās bioloģijas metodes

2015

Pētījuma mērķis bija noteikt Alaria alata sastopamību dažādās infrapopulācijās Latvijā un veikt ģints filoģenētisko koku analīzi. Pētījuma gaitā tika noteikta A. alata sastopamība meža cūkās (77%), lapsās (88%) un jenotsuņos (84%). Baltijas valstīs ekstensitāte definitīvajos saimniekos ir līdzīga. Salīdzinot divas mezocerkāriju klātbūtnes noteikšanas metodes, tika konstatēts, ka, pielietojot Alaria migrācijas metodi, mezocerkārijus konstatē 10 reizes biežāk salīdzinot ar līdz šim izmantoto metodi. Kā piemērotākais A. alata DNS izdalīšanas reaģentu komplekts tika atzīts DNeasy Blood&Tissue Kit ar palielinātu proteināzes K daudzumu (25μl) un pagarinātu inkubācijas laiku (līdz 15h). Konstruējo…

Rituximab for managing relapsing or refractory patients with idiopathic thrombotic thrombocytopenic purpura – haemolytic uraemic syndrome

2010

Framgångsrik karriärkvinna : en undersökning av kvinnobilden i finska och svenska Elle

2011

Työn tavoitteena oli tutkia naiskuvaa suomalaisessa ja ruotsalaisessa Elle-lehdessä kielen kautta. Työssä käytiin läpi lehtien artikkelit kielellisinä kokonaisuuksina sekä vertailtiin artikkeleita keskenään, jotta löydettiin mahdolliset kulttuurilliset erot naiskuvassa. Metodina käytettiin laadullista ja määrällistä sisällönanalyysiä. Tutkimusaineisto koostui vuoden 2009 lokakuun ja marraskuun numeroista. Yhteensä analysoitiin neljä lehteä. Tutkimuksen kokonaisuuden muodostivat yli kaksisivuiset artikkelit. Kuvamateriaali jätettiin pois analyysista. Analyysin tavoitteena oli tutkia kuinka Elle-nainen esitetään lehdessä. Tutkimme millaisessa roolissa naiset esiintyivät artikkeleissa sekä kui…

Patterns of changes of anti‐ADAMTS13 after plasma exchange

2006

An enzyme-linked immunosorbent assay (ELISA) has recently been developed to detect antibodies against the von Willebrand factor-cleaving protease ADAMTS13 in patients with thrombotic thrombocytopenic purpura (TTP). The ELISA is based on incubation of plasma with immobilized recombinant ADAMTS13 followed by visualization of IgG and IgM antibodies by means of secondary enzyme-labeled antibodies [1]. In a recent study, anti-ADAMTS13 IgG antibodies were detected in most patients with TTP (97%) characterized by severe ADAMTS13 deficiency (< 10% of normal) [1]. The ELISA was more sensitive than the standard inhibitor assay based upon ADAMTS13 neutralizing activity, which gave positive results in …