Search results for "AREs"

showing 10 items of 1717 documents

NOseq: amplicon sequencing evaluation method for RNA m6A sites after chemical deamination

2020

Abstract Methods for the detection of m6A by RNA-Seq technologies are increasingly sought after. We here present NOseq, a method to detect m6A residues in defined amplicons by virtue of their resistance to chemical deamination, effected by nitrous acid. Partial deamination in NOseq affects all exocyclic amino groups present in nucleobases and thus also changes sequence information. The method uses a mapping algorithm specifically adapted to the sequence degeneration caused by deamination events. Thus, m6A sites with partial modification levels of ∼50% were detected in defined amplicons, and this threshold can be lowered to ∼10% by combination with m6A immunoprecipitation. NOseq faithfully d…

AdenosineSequence analysisAcademicSubjects/SCI00010Bisulfite sequencingDeaminationAdenosine/analogs & derivatives; Adenosine/analysis; Algorithms; Animals; Chromatography Liquid; Deamination; Drosophila melanogaster/genetics; HEK293 Cells; HeLa Cells; High-Throughput Nucleotide Sequencing/methods; Humans; RNA/chemistry; RNA Long Noncoding/chemistry; RNA Messenger/chemistry; RNA Ribosomal 18S/chemistry; Sequence Alignment; Sequence Analysis RNA/methods; Tandem Mass SpectrometrySequence alignmentComputational biologyBiology010402 general chemistry[SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology01 natural sciencesTranscriptome03 medical and health sciencesNarese/13Tandem Mass Spectrometry[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]GeneticsRNA Ribosomal 18SAnimalsHumansRNA MessengerComputingMilieux_MISCELLANEOUS030304 developmental biology0303 health sciencesSequence Analysis RNARNAHigh-Throughput Nucleotide Sequencing[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biologyAmpliconRibosomal RNA0104 chemical sciencesDrosophila melanogasterHEK293 CellsDeaminationMethods OnlineRNA[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]RNA Long NoncodingSequence AlignmentAlgorithmsChromatography LiquidHeLa Cells
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Dysarthria in acute ischemic stroke: lesion topography, clinicoradiologic correlation, and etiology.

2001

Background and purpose: Although dysarthria is a frequent symptom in cerebral ischemia, there is little information on its anatomic specificity, spectrum of associated clinical characteristics, and etiologic mechanisms. Methods: An investigation of 68 consecutive patients with sudden onset of dysarthria due to a single infarction confirmed by MRI or CT was conducted. Results: Dysarthria was associated with a classic lacunar stroke syndrome in 52.9% of patients. Isolated dysarthria and dysarthria–central facial and lingual paresis occurred in 2.9% (n = 2) and 10.3% (n = 7), respectively. Dysarthria–clumsy hand syndrome was observed in 11.7% (n = 8) of patients and associated with pure motor …

AdultBrain InfarctionMalePathologymedicine.medical_specialtyLacunar strokeInternal capsuleBrain IschemiaDysarthriaInternal Capsulemedicine.arteryInternal medicineCerebellumCentrum semiovalemedicineHumansSuperior cerebellar arteryAgedRetrospective StudiesAged 80 and overChi-Square Distributionbusiness.industryCerebral infarctionCerebral peduncleDysarthriaMiddle Agedmedicine.diseasenervous system diseasesHemiparesisIschemic Attack TransientCardiologyFemaleNeurology (clinical)medicine.symptombusinessNeurology
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Realignment of myocardial first-pass MR perfusion images using an automatic detection of the heart-lung interface

2004

International audience; Abstract: Magnetic resonance first-pass imaging of a bolus of contrast agent is well adapted to distinguish normal and hypoperfused areas of the myocardium. In most cases, the signal intensity-time curves in user defined regions of interest (ROI) are studied. As image acquisition is ECG-gated, the images are acquired at the same moment in the cardiac cycle, and the basic shape of the heart is similar from one view to the next. However, superficial respiratory motion can displace the heart in the short-axis plane. The aim of this study is to correct for the respiratory motion of the heart by performing an automatic realignment of the myocardial ROI based on a method t…

AdultGadolinium DTPAMaleComputer scienceInterface (computing)media_common.quotation_subjectMyocardial InfarctionBiomedical EngineeringBiophysicsContrast MediaImage registration030204 cardiovascular system & hematologySignal030218 nuclear medicine & medical imaging03 medical and health sciences0302 clinical medicineCoronary CirculationImage Processing Computer-Assisted[INFO.INFO-IM]Computer Science [cs]/Medical ImagingmedicineHumansmagnetic resonance imagingContrast (vision)Radiology Nuclear Medicine and imagingComputer visionLeast-Squares AnalysisLungAgedmedia_commonLung[ INFO.INFO-IM ] Computer Science [cs]/Medical ImagingCardiac cyclemedicine.diagnostic_testMr perfusionbusiness.industrycontrast-enhanced first-passHeartMagnetic resonance imagingMiddle Agedimage registrationmedicine.anatomical_structureFemaleArtificial intelligencebusinessmyocardial perfusionMagnetic Resonance Imaging
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Does higher education protect against obesity? Evidence using Mendelian randomization

2017

Objectives. The aim of this explorative study was to examine the effect of education on obesity using Mendelian randomization. Methods. Participants (N = 2011) were from the on- going nationally representative Young Finns Study (YFS) that began in 1980 when six cohorts (aged 30, 33, 36, 39, 42 and 45 in 2007) were recruited. The average value of BMI (kg/m(2)) measurements in 2007 and 2011 and genetic information were linked to comprehensive register based information on the years of education in 2007. We first used a linear regression (Ordinary Least Squares, OLS) to estimate the relationship between education and BMI. To identify a causal relationship, we exploited Mendelian randomization …

AdultMale0301 basic medicinemedicine.medical_specialtyobesityschoolingEpidemiology515 PsychologyProtective factorBody Mass Index03 medical and health sciencesbody weightBMI0302 clinical medicineWaist–hip ratioInternal medicineLinear regressionMendelian randomizationmedicineHumans030212 general & internal medicineFinlandGenetic associationeducationbusiness.industryPublic Health Environmental and Occupational Healthta3141ylipainota3142Mendelian Randomization Analysista3121medicine.diseaseObesityConfidence interval030104 developmental biologyEndocrinologykoulutusOrdinary least squaresEducational StatuslihavuusFemalewaist-hip ratiobusinessGenome-Wide Association StudyDemographyPreventive Medicine
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Effectiveness of Mirror Therapy for Subacute Stroke in Relation to Chosen Factors.

2016

Purpose The aim of this study was to determine the effectiveness of mirror therapy (MT) combined with comprehensive treatment and to investigate the possible relationships of functional state. Design Prospective, controlled trial of 60 stroke inpatients. Methods The Functional Index "Repty" (FIR) was an outcome measure to assess changes of independence in daily activities. The Frenchay Arm Test (FAT) and Motor Status Score were outcome measures to assess changes in hand function. Findings The analysis of pre- and posttest data indicated a significant improvement in hand function ([INCREMENT]FAT in the Mirror group p = .035, N = 30). Age factor indicated a significant change in relation to F…

AdultMale030506 rehabilitationmedicine.medical_specialtyActivities of daily livingSubacute strokePhysical Therapy Sports Therapy and RehabilitationRehabilitation Nursinglaw.invention03 medical and health sciences0302 clinical medicinePhysical medicine and rehabilitationRandomized controlled triallawFeedback SensoryActivities of Daily LivingmedicineHumansClinical significanceProspective StudiesProspective cohort studyStrokeolder adultsGeneral NursingPhysical Therapy ModalitiesParesisAgedRehabilitationGeneral MedicineMiddle Agedmedicine.diseasemobilityParesisPatient Outcome AssessmentStrokeMirror therapyPhysical therapyFemalemedicine.symptom0305 other medical sciencePsychology030217 neurology & neurosurgeryRehabilitation nursing : the official journal of the Association of Rehabilitation Nurses
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Cross-sectional study of quadriceps properties and postural stability in patients with chronic stroke and limited vs. non-limited community ambulatio…

2019

Background: Changes in the paretic-side metabolism post-stroke and quadriceps muscle mechanical properties favour muscle wasting, affecting postural instability and walking impairment. Further clar...

AdultMale030506 rehabilitationmedicine.medical_specialtyCross-sectional studyWalkingQuadriceps Muscle03 medical and health sciences0302 clinical medicinePhysical medicine and rehabilitationIsometric ContractionmedicineHumansIn patientMuscle StrengthMuscle SkeletalWastingStrokeChronic strokePostural BalanceBalance (ability)AgedUltrasonographyCommunity and Home CareAged 80 and overbusiness.industryRehabilitationQuadriceps muscleMiddle Agedmedicine.diseaseWalking SpeedParesisStrokeCross-Sectional StudiesThighPostural stabilityFemalesense organsNeurology (clinical)Independent Livingmedicine.symptom0305 other medical sciencebusinessSkin Temperature030217 neurology & neurosurgeryTopics in stroke rehabilitation
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Functional anatomy of motor recovery after early brain damage

2004

Functional magnetic resonance imaging and transcranial magnetic stimulation were used to examine a 34 year-old right-handed patient, who, at the age of 6 years, had experienced sudden right hemiplegia, seizures, and stupor during a bout of measles encephalitis, followed by incomplete distal right motor recovery. Morphological MRI showed massive unilateral enlargement of the left ventricle, associated with extreme thinning of the white and gray matter, with partial preservation of the pyramidal tract. Functional MRI and transcranial magnetic stimulation revealed reorganization of the motor cortices, and integrity of the corticospinal pathway, respectively. Our findings indicate that complete…

AdultMaleCORTEXmedicine.medical_treatmentCHILDHOODAdult; Brain Damage Chronic; Humans; Magnetic Resonance Imaging; Magnetoencephalography; Male; Paresis; Psychomotor Performance; Subacute Sclerosing PanencephalitisCHILDRENBrain damageArts and Humanities (miscellaneous)Magnetic Resonance Imaging; Magnetoencephalography; Paresis; Humans; Brain Damage Chronic; Adult; Psychomotor Performance; Subacute Sclerosing Panencephalitis; MalemedicineHumansBrain DamagePLASTICITYChronicPyramidal tractsmedicine.diagnostic_testSettore M-PSI/02 - Psicobiologia E Psicologia FisiologicaStuporMagnetoencephalographyAnatomymedicine.diseaseMagnetic Resonance ImagingTranscranial magnetic stimulationParesismedicine.anatomical_structureVentricleBrain Damage ChronicMotor recoverySettore MED/26 - NeurologiaNeurology (clinical)Subacute Sclerosing Panencephalitismedicine.symptomFunctional magnetic resonance imagingPsychologyNeuroscienceEncephalitisSTROKEPsychomotor PerformanceMRI
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k-Nearest neighbour local linear prediction of scalp EEG activity during intermittent photic stimulation

2011

The characterization of the EEG response to photic stimulation (PS) is an important issue with significant clinical relevance. This study aims to quantify and map the complexity of the EEG during PS, where complexity is measured as the degree of unpredictability resulting from local linear prediction. EEG activity was recorded with eyes closed (EC) and eyes open (EO) during resting and PS at 5, 10, and 15. Hz in a group of 30 healthy subjects and in a case-report of a patient suffering from cerebral ischemia. The mean squared prediction error (MSPE) resulting from k-nearest neighbour local linear prediction was calculated in each condition as an index of EEG unpredictability. The linear or …

AdultMaleComputer sciencePhotic StimulationBiomedical EngineeringBiophysicsElectroencephalographyEyeMachine learningcomputer.software_genreBrain IschemiaYoung AdultIschemiamedicineHumansEEGPredictabilityIntermittent photic stimulationK nearest neighbourPredictability mapAgedScalpLocal linearmedicine.diagnostic_testbusiness.industrySpectrum AnalysisLocal linear predictionElectroencephalographySignal Processing Computer-AssistedPattern recognitionScalp eegmedicine.anatomical_structureScalpSettore ING-INF/06 - Bioingegneria Elettronica E InformaticaCortexLinear ModelsFemaleArtificial intelligencebusinesscomputerPhotic StimulationMedical Engineering & Physics
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Education Leads to a More Physically Active Lifestyle : Evidence Based on Mendelian Randomization

2019

Physical inactivity is a major health risk worldwide. Observational studies suggest that higher education is positively related to physical activity, but it is not clear whether this relationship constitutes a causal effect. Using participants (N = 1651) drawn from the Cardiovascular Risk in Young Finns Study linked to nationwide administrative data from Statistics Finland, this study examined whether educational attainment, measured by years of education, is related to adulthood physical activity in terms of overall physical activity, weekly hours of intensive activity, total steps per day, and aerobic steps per day. We employed ordinary least squares (OLS) models and extended the analysis…

AdultMaleEvidence-based practiceHigher educationregister‐based dataväestötutkimusphysical activityPhysical Therapy Sports Therapy and Rehabilitation030204 cardiovascular system & hematologyliikuntaEducation03 medical and health sciences0302 clinical medicinekoulutustasoMendelian randomizationGlobal healthMendelian randomizationMedicineHumansOrthopedics and Sports MedicineLongitudinal Studies315 Sport and fitness sciencesExerciseLife StyleelämäntapaFinlandväestötietojärjestelmäteducationbusiness.industryInstrumental variable030229 sport sciencesMendelian Randomization AnalysisMiddle Aged3142 Public health care science environmental and occupational healthEducational attainmentCross-Sectional StudieskoulutusOrdinary least squaresObservational studyFemalebusinessfyysinen aktiivisuusDemography
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Work, family and daily mobility: a new approach to the problem through a mobility survey

2013

Objectives: To analyze gender inequalities in socioeconomic factors affecting the amount of time spent travelling for work-related and home-related reasons among working individuals aged between 30 and 44 years old during a weekday in Catalonia (Spain). Methods: A cross-sectional study was conducted. Data were obtained from employed individuals aged between 30 and 44 years of age who reported travelling on the day prior to the interview in the Catalan Mobility Survey 2006 (N = 23,424). Multivariate logistic regression models were adjusted to determine the factors associated with longer time spent travelling according to the reason for travelling (work- or home-related journeys). Odds ratios…

AdultMaleGerontologyWorkTime FactorsInequalitymedia_common.quotation_subjectTransportationGender rolesLogistic regressionPublic policiesSex FactorsPolíticas públicasHumansWorking populationFamilyDesigualdadesSocioeconomic statusmedia_commonRoles de géneroPublic Health Environmental and Occupational HealthFamily relationsOdds ratioTransporteConfidence intervalTravel timeCross-Sectional StudiesSocioeconomic FactorsWork (electrical)FemaleInequalitiesPsychologyhuman activitiesDemographyRelaciones familiares
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