Search results for "ARTICLES"
showing 10 items of 9626 documents
Protein-membrane interaction probed by single plasmonic nanoparticles.
2008
We present a nanosized and addressable sensor platform based on membrane coated plasmonic particles and show unequivocally the covering with lipid bilayers as well as the subsequent detection of streptavidin binding to biotinylated lipids. The binding is detected on membrane covered gold nanorods by monitoring the spectral shift by fast single particle spectroscopy (fastSPS) on many particles in parallel. Our approach allows for local analysis of protein interaction with biological membranes as a function of the lateral composition of phase separated membranes.
The Sensor Kinase DctS Forms a Tripartite Sensor Unit with DctB and DctA for Sensing C4-Dicarboxylates in Bacillus subtilis
2013
The DctSR two-component system of Bacillus subtilis controls the expression of the aerobic C4-dicarboxylate transporter DctA. Deletion of DctA leads to an increased dctA expression. The inactivation of DctB, an extracellular binding protein, is known to inhibit the expression of dctA. Here, interaction between the sensor kinase DctS and the transporter DctA as well as the binding protein DctB was demonstrated in vivo using streptavidin (Strep) or His protein interaction experiments (mSPINE or mHPINE), and the data suggest that DctA and DctB act as cosensors for DctS. The interaction between DctS and DctB was also confirmed by the bacterial two-hybrid system (BACTH). In contrast, no indicati…
Stress management strategies and quality of life in undergraduate nursing and midwifery students in Poland: A pilot study
2022
Abstract Aim To establish the level of the quality of life (QOL) and its determinants in nursing and midwifery students, evaluate stress management strategies and find the correlation between the frequency of specific strategies and the QOL. Design Diagnostic survey. Methods The study was conducted in June 2019. Two hundred thirty‐four undergraduate students were examined with the use of Mini‐Cope Inventory of Stress Management Measurement (Mini‐Cope) and World Health Organization Quality of Life Instrument Brief Form. Results The average QOL of the respondents was found as good (4.06 ± 0.73). The students graded their QOL in the social relationship domain the highest (15.98 ± 2.97) and the…
30 Years on: How the Neurodevelopmental Hypothesis of Schizophrenia Morphed into the Developmental Risk Factor Model of Psychosis
2017
At its re-birth 30 years ago, the neurodevelopment hypothesis of schizophrenia focussed on aberrant genes and early neural hazards, but then it grew to include ideas concerning aberrant synaptic pruning in adolescence. The hypothesis had its own stormy development and it endured some difficult teenage years when a resurgence of interest in neurodegeneration threatened its survival. In early adult life, it over-reached itself with some reductionists claiming that schizophrenia was simply a neurodevelopmental disease. However, by age 30, the hypothesis has matured sufficiently to incorporated childhood and adult adversity, urban living and migration, as well as heavy cannabis use, as importan…
Poly(γ-Glutamic Acid) as an Exogenous Promoter of Chondrogenic Differentiation of Human Mesenchymal Stem/Stromal Cells
2015
Cartilage damage and/or aging effects can cause constant pain, which limits the patient's quality of life. Although different strategies have been proposed to enhance the limited regenerative capacity of cartilage tissue, the full production of native and functional cartilaginous extracellular matrix (ECM) has not yet been achieved. Poly(γ-glutamic acid) (γ-PGA), a naturally occurring polyamino acid, biodegradable into glutamate residues, has been explored for tissue regeneration. In this work, γ-PGA's ability to support the production of cartilaginous ECM by human bone marrow mesenchymal stem/stromal cells (MSCs) and nasal chondrocytes (NCs) was investigated. MSC and NC pellets were cultur…
A Dominance Variant Under the Multi-Unidimensional Pairwise-Preference Framework: Model Formulation and Markov Chain Monte Carlo Estimation.
2018
Forced-choice questionnaires have been proposed as a way to control some response biases associated with traditional questionnaire formats (e.g., Likert-type scales). Whereas classical scoring methods have issues of ipsativity, item response theory (IRT) methods have been claimed to accurately account for the latent trait structure of these instruments. In this article, the authors propose the multi-unidimensional pairwise preference two-parameter logistic (MUPP-2PL) model, a variant within Stark, Chernyshenko, and Drasgow’s MUPP framework for items that are assumed to fit a dominance model. They also introduce a Markov Chain Monte Carlo (MCMC) procedure for estimating the model’s paramete…
Automated electron diffraction tomography – development and applications
2019
Electron diffraction tomography, a potential method for structure analysis of nanocrystals, and, in more detail, the strategies to use automated diffraction tomography (ADT) technique are described. Examples of ADT application are discussed according to the material class.
Cosmological origin of anomalous radio background
2012
The ARCADE 2 collaboration has reported a significant excess in the isotropic radio background, whose homogeneity cannot be reconciled with clustered sources. This suggests a cosmological origin prior to structure formation. We investigate several potential mechanisms and show that injection of relativistic electrons through late decays of a metastable particle can give rise to the observed excess radio spectrum through synchrotron emission. However, constraints from the cosmic microwave background (CMB) anisotropy, on injection of charged particles and on the primordial magnetic field, present a challenge. The simplest scenario is with a greater than or similar to 9 GeV particle decaying i…
Structure finding in cosmological simulations: the state of affairs
2013
The ever increasing size and complexity of data coming from simulations of cosmic structure formation demands equally sophisticated tools for their analysis. During the past decade, the art of object finding in these simulations has hence developed into an important discipline itself. A multitude of codes based upon a huge variety of methods and techniques have been spawned yet the question remained as to whether or not they will provide the same (physical) information about the structures of interest. Here we summarize and extent previous work of the "halo finder comparison project": we investigate in detail the (possible) origin of any deviations across finders. To this extent we decipher…
Late decaying 2-component dark matter scenario as an explanation of the AMS-02 positron excess
2016
The long standing anomaly in the positron flux as measured by the PAMELA and AMS-02 experiments could potentially be explained by dark matter (DM) annihilations. This scenario typically requires a large "boost factor" to be consistent with a thermal relic dark matter candidate produced via freeze-out. However, such an explanation is disfavored by constraints from CMB observations on energy deposition during the epoch of recombination. We discuss a scenario called late-decaying two-component dark matter (LD2DM), where the entire DM consists of two semi-degenerate species. Within this framework, the heavier species is produced as a thermal relic in the early universe and decays to the lighter…