Search results for "ATG"
showing 10 items of 908 documents
Systemic Oxidative Stress and Visceral Adipose Tissue Mediators of NLRP3 Inflammasome and Autophagy Are Reduced in Obese Type 2 Diabetic Patients Tre…
2020
Obesity is a low-grade inflammatory condition affecting a range of individuals, from metabolically healthy obese (MHO) subjects to type 2 diabetes (T2D) patients. Metformin has been shown to display anti-inflammatory properties, though the underlying molecular mechanisms are unclear. To study whether the effects of metformin are mediated by changes in the inflammasome complex and autophagy in visceral adipose tissue (VAT) of obese patients, a biopsy of VAT was obtained from a total of 68 obese patients undergoing gastric bypass surgery. The patients were clustered into two groups: MHO patients and T2D patients treated with metformin. Patients treated with metformin showed decreased levels o…
Imaging Noncanonical Autophagy and LC3-Associated Phagocytosis in Cultured Cells
2019
International audience; Monitoring of ATG8 proteins by western blotting and immunofluorescence microscopy are the most common methods to monitor the autophagy pathway. However, it has recently been shown that ATG8 proteins can be lipidated to non-autophagosome, single-membrane compartments through a noncanonical autophagy pathway. This is commonly found to occur during macro-endocytic processes such as phagocytosis, where it has been termed LC3-associated phagocytosis, and upon lysosomotropic drug treatment. Therefore, care is required when interpreting data based on ATG8 in order to conclude whether a signal relates to the canonical or noncanonical pathway. Here we provide methods to monit…
Validation of Using Smartphone Built-In Accelerometers to Estimate the Active Energy Expenditures of Full-Time Manual Wheelchair Users with Spinal Co…
2021
[EN] This study aimed to investigate the validity of using built-in smartphone accelerometers to estimate the active energy expenditures of full-time manual wheelchair users with spinal cord injury (SCI). Twenty participants with complete SCI completed 10 5-min daily activities that involved the upper limbs, during which their oxygen consumption and upper limb activity were registered using a portable gas analyzer and a smartphone (placed on the non-dominant arm), respectively. Time series of 1-min averaged oxygen consumption and 55 accelerometer variables (13 variables for each of the four axes and three additional variables for the correlations between axes) were used to estimate three mu…
Artefact co-construction in virtual exchange: 'Youth Entrepreneurship for Society'
2020
The aim of this research study around the co-construction and sharing of authentic and meaningful artefacts within the 'Youth Entrepreneurship for Society' virtual exchange was to find out how the implementation of artefact-based telecollaborative tasks fosters 21st competences and social entrepreneurship in English for Specific Purposes settings. The project tasks were informed by constructionism and supported virtual collaboration an effort to promote social justice. They revolved around social entrepreneurship in English for Specific Purposes contexts, aiming to connect the classroom with the outside world through the involvement of local organisations, while fostering students' twenty-f…
Researching Identity and L2 Pragmatics in Digital Stories: A Relational Account.
2020
This study explores college EFL learners' construction of identity through the analysis of their pragmatic choices in digital stories, in which they narrated their relationship with another person they had helped in the past. More specifically, such choices were examined following Relational Dialectics Theory in learners' enactments of "connection" with and "autonomy" from this person. A specific view of identity in language education, the notion of "relational work" in (im)politeness research, and a social semiotic framework were also employed in data analysis. Learners' pragmatic choices ranged from the selection of the topic of their narratives according to types of social bonds, to the …
Translating Teacher Funds of Identity into Curricular Proposals for the EFL Classroom: A Model for Student-Teacher Innovation and Professional Develo…
2019
This article describes the progress and findings of a research project, organized in the context of a university Masters course in Teaching English as a Foreign Language (TEFL), which aimed at translating the student-teachers' (STs) funds of identity into worthwhile curricular proposals for the English as a Foreign Language (EFL) subject. The first part of the research involved 38 STs in three workshops through which they became aware of their own funds of identity, expanded their own teacher identity, and finally designed curricular proposals that incorporated their funds of identity as valuable pedagogical resources. The second part of the research took place during the two-month practicu…
La variación genérico-discursiva de la atenuación como resultado de la variación de la imagen
2018
AbstractoEl presente trabajo investiga en qué medida la atención a la imagen social incide en la expresión de la atenuación. Para ello se realiza un estudio de los mecanismos atenuantes en cinco géneros discursivos seleccionados por su diferente tratamiento de la imagen: artículos de investigación, foros de comunicación en línea, conversaciones coloquiales, mesas redondas de discusión de expertos y debates políticos. Los resultados muestran variación tanto en las formas empleadas como en las funciones atenuantes, de acuerdo con las diferentes necesidades de imagen que origina cada género. Ello constituye un fuerte argumento a favor de la implicación de la noción de imagen en la caracterizac…
Teaching English as a Non-Imperial Language in an Underprivileged Public School in Spain
2018
This article summarizes the processes and findings of a 2-year collaborative action research (CAR) project that analyzed and aimed to counteract some of the most negative educational effects of English linguistic imperialism in the field of English language teaching (ELT) and, more concretely, in the context of English as a foreign language education in Spain. The CAR investigated the ramifications of this phenomenon in a primary school located in one of the most disadvantaged neighborhoods in the city of València. The pedagogical alternative it embraced in order to reverse the underlying tenets of ELT under present-day neoliberal imperialism consisted in combining art and multimodality thr…
Formulating 'principles of procedure' for the foreign language classroom: A framework for process model language curricula
2015
This article aims to apply Stenhouse's process model of curriculum to foreign language (FL) education, a model which is characterized by enacting principles of procedure which are specific to the discipline which the school subject belongs to. Rather than to replace or dissolve current approaches to FL teaching and curriculum development, this article seeks to improve and enrich communicative and task-based orientations with an additional criterion for assessing the educational worth of the tasks through which these orientations are developed. Unlike the objectives and competences models, principles of procedure provide an intrinsic justification of school curriculum by enacting the epistem…
Teachers’ perspectives on telecollaboration in secondary school foreign language education
2018
In recent years, telecollaboration has proved to be a useful tool in the acquisition of foreign languages and intercultural communicative skills (Belz 2003b; Clavel-Arroitia, Pennock-Speck 2015a, 2015b; Hewitt, Brett 2007; O’Dowd 2007; Su et al., 2005). This paper focuses on how prepared secondary-school teachers believe they are in order to successfully implement telecollaboration in the classroom. To gather information on their views we carried out an online survey of 179 secondary school foreign language teachers and a series of focus group interviews in the context of a European project, TeCoLa. The advantage of the double- pronged approach to data collection, quantitative and qualitati…